Bacterial cellulose (BC) has many good qualities: high purity, water absortion, plasticity… BC can be served as matrix absorbing cultural medium for the growth of microbial, plant and [r]
(1)Utilisation of bacterial cellulose (BC) as support matrix for the cultivation of microbial, plant and animal cells
Le Thi Thanh Loan and Pham Thanh Ho*
Department of Plant Biotechnology and Bioconversion, University of Sciences – Vietnam National University - Ho Chi Minh, 227 Nguyen Van Cu, Ho Chi Minh, Vietnam
ABSTRACT
Bacterial cellulose (BC) has many good qualities: high purity, water absortion, plasticity… BC can be served as matrix absorbing cultural medium for the growth of microbial, plant and animal cells on the surface The experimental results showed that all the types of these cells normally grow on BC medium
Microorganisms
– Two strains Azotobacter have the nitrogen fixation ability and biomass production with the maximum after days (582,68 mg/ml and 423,67 mg/ml)
– The yeast strains, such as Saccharomyces cerevisiae, S carlsbergensis, and Rhodotorula sp normally developed and gave the biomass on BC surface better in comparation with the culture in liquid medium
– The biomass production of Chlorella vulgaris and strains of Spirulina platensis are also good on BC surface
The primary results of this research confirm the possibilities of BC utilisation as matrix surface for the cultivation of different microorganisms The method for biomass production on BC surface has many advantages:
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(2)– The less energy consumption for aeration, because the microbial cells on the BC surface directly contacted with the air
– The productivity of biomass production is good, biomass recovery is more simple and the water separation is easier (no need for water separation by centrifugation or filtration) – The waste water after fermentation is limited
Plant tissue cultures
BC was used as one of the supporter systems in plant tissue cultures The results of shoot cultures of Nicotiana tabacum, Musa sp., Ficus carica, Vetiveria zizanioides were reported On the medium with BC supporter, the shoots of N tabacum and V zizanioides grew better than those on agar supporter 1,3 and 1,4 times respectively for weeks of culture F carica shoots on the BC medium were about cm in height while those on the agar medium were about 1.4 cm after weeks of culture Musa shoots on the BC medium grew as well as those on the agar medium The experiments showed that BC was a good supporter in cultures of Nicotiana, Vetiveria, Ficus and Musa
The culture of animal cells