Nguyen Thi Minh Thu - overview of these amorphogenesis inducing agent and their interactions with cellulose

overview of these amorphogenesis inducing agent and their interactions with cellulose Name: Nguyen Thi Minh Thu 2016.11.18 CONTENTS Overview The amorphogenesis – inducing proteins 2.1 Expansin 2.2 EXLX1 or YOAj 2.3 Swollenin Overview Amorphogenesis reducing The amorphogenesis inducing or disrupting is disrupted or loosened by non – hydrolytic proteins at inaccessible regions in enzymatic saccharification of cellulose What is the amorphogenesis inducing agent brings disrupting or loosening of the plant cell wall ? To disrupt and weaken the plant cell wall without releasing a significant amount of soluble sugars Expansin The amorphogenesis – inducing EXLX1 protein Promoting amorphegenesis of the substrate Swollenin 2.1 Expansin  Expansin are plant cell – wall loosening proteins involved in cell enlargement and in a variety of other developmental processes in which cell – wall modification occurs  The structure of expansin includes two domains (domain and domain 2) preceded by a signal peptide • SP Domain Domain lacks of a second asparate so not be Expansin hydrolytic activity A G2A Domain likely the structure of GH45 It C B F • Domain was initially speculated tobe a CBM on the basic of conserved aromatic and polar residues on the surface of the protein p12 Barwin To induce extension of isolated cell walls To be associated with cell – wall loosening in growing cells Urea and deuterium oxide reduces the creep rate on cell wall glycans 2.2 EXLX1 OR YOAJ • • is an expansin from Bacterial Likely the structure of plant expansins, consist of two domains: + Domain 1: form a six – stranded double – ψ β - barrel with high structural similarity to D1 of plant expansins, much lower similarity to GH – 102 peptidoglycan lytic transglycosylases + Domain 2: is structurally related to domain D2 plant expansins, forming an Ig – like β sandwich • It induces extension of plant cell walls, but it has weak expansin activity 2.3 SWOLLENIN   Is a special expansin – like protein from fungus Trichoderma reesei The structure of swollenin includes an N – terminal cellulose – specific carbohydrate binding module (CBM) connect via a linker region to a C – terminal expansin – like domain (mammalian fibronectin type III – like repeats) Promoted the disruption of cellulosic biomass through a non – hydrolytic weakening of hydrogen bonding Separated relatively crystalline regions of cellulose from each other THANK YOU