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Mức độ biểu hiện của các gen CaTCP trong lá và rễ của cây đậu gà trong điều kiện khử nước và xử lý ABA .... TF Transcription factor Yếu tố phiên mã TF TCP Transcription Factor Teosinte B
BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ NÔNG NGHIỆP VÀ PTNT VIỆN KHOA HỌC NÔNG NGHIỆP VIỆT NAM TRẦN DUY CƯỜNG NGHIÊN CỨU HỌ GEN MÃ HOÁ NHÂN TỐ PHIÊN MÃ TCP TRONG ĐIỀU KIỆN BẤT THUẬN PHI SINH HỌC Ở CÂY ĐẬU CICER ARIETINUM LUẬN ÁN TIẾN SỸ NÔNG NGHIỆP HÀ NỘI - 2023 BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ NÔNG NGHIỆP VÀ PTNT VIỆN KHOA HỌC NÔNG NGHIỆP VIỆT NAM TRẦN DUY CƯỜNG NGHIÊN CỨU HỌ GEN MÃ HOÁ NHÂN TỐ PHIÊN MÃ TCP TRONG ĐIỀU KIỆN BẤT THUẬN PHI SINH HỌC Ở CÂY ĐẬU CICER ARIETINUM Chuyên ngành: Công nghệ sinh học Mã số: 9420201 LUẬN ÁN TIẾN SỸ NÔNG NGHIỆP Người hướng dẫn khoa học: PGS.TS Trần Đăng Khánh TS Trần Phan Lam Sơn HÀ NỘI - 2023 i LỜI CAM ĐOAN Tôi xin cam đoan cơng trình nghiên cứu hướng dẫn PGS.TS Trần Đăng Khánh TS Trần Phan Lam Sơn Trong luận án này, kết thực trung thực, phần nội dung cơng bố Tạp chí khoa học quốc tế uy tín nhận đồng ý tất tác giả báo Các nội dung luận văn chưa sử dụng để công bố cơng trình nghiên cứu để nhận học vị khác Tồn trích dẫn ghi rõ nguồn gốc Hà Nội, ngày tháng Trần Duy Cường năm 2023 ii LỜI CẢM ƠN Đầu tiên, xin gửi lời cảm ơn chân thành tới chương trình đào tạo hợp tác quốc tế IPA (International Program Associate) liên kết Viện Khoa học Nông nghiệp Việt Nam (VAAS, Việt Nam) viện nghiên cứu RIKEN Nhật Bản Trong suốt trình học tập làm việc, người giúp đỡ tạo điều kiện tốt để tơi hồn thành luận án Tiếp theo, xin gửi lời cảm ơn chân thành tới PGS.TS Trần Đăng Khánh – Trưởng môn Kỹ Thuật Di truyền – Viện Di truyền Nông nghiệp TS Trần Phan Lam Sơn, nguyên trưởng Nhóm nghiên cứu đáp ứng với yếu tố bất lợi – Trung tâm Khoa học nguồn tài nguyên bền vững, Viện RIKEN, Nhật Bản Các thầy hướng dẫn tận tình bảo, giúp đỡ với điều kiện tốt để hồn thành cơng trình nghiên cứu luận án Bên cạnh đó, tơi xin gửi lời cảm ơn sâu sắc tới thành viên thuộc Nhóm nghiên cứu đáp ứng với yếu tố bất lợi TS Trần Phan Lam Sơn Mọi người hướng dẫn chi tiết tạo điều kiện để tơi hồn thành cơng trình nghiên cứu Hơn nữa, xin gửi lời cảm ơn chân thành tới Ban Đào tạo Sau đại học – Viện Khoa học Nông nghiệp Việt Nam lãnh đạo viện Di truyền Nông nghiệp tạo điều kiện tốt cho để hồn thành tốt khố học hồn thiện luận án Cuối cùng, xin gửi lời cảm ơn tới người thân gia đình tơi, người luôn động viên từ ngày đầu bắt đầu khố học lúc tơi hồn thành cơng trình nghiên cứu luận án Bên cạnh đó, tơi xin chân thành cảm ơn bạn bè, anh chị em đồng nghiệp ln ủng hộ tơi suốt q trình học tập Nhật Bản thời gian hoàn thiện luận án Việt Nam Hà Nội, ngày tháng năm 2023 Trần Duy Cường iii MỤC LỤC LỜI CAM ĐOAN i LỜI CẢM ƠN ii MỤC LỤC iii DANH MỤC CHỮ VIẾT TẮT vii DANH MỤC BẢNG x DANH MỤC HÌNH xi MỞ ĐẦU Chương I: TỔNG QUAN TÀI LIỆU 1.1 Tổng quan đậu gà 1.1.1 Nguồn gốc, phân bố đậu gà 1.1.2 Đặc điểm hình thái đậu gà 1.1.3 Phân loại đậu gà 1.1.4 Đa dạng di truyền đậu gà 1.1.5 Giá trị dinh dưỡng đậu gà 1.2 Các nghiên cứu đậu gà 10 1.2.1 Chọn tạo giống đậu gà mang đặc điểm mong muốn 10 1.2.2 Tình hình sản xuất đậu gà 22 1.3 Họ nhân tố phiên mã TCP 24 1.3.1 Nghiên cứu nhân tố phiên mã thực vật 24 1.3.2 Họ nhân tố phiên mã TCP 26 1.3.3 Vai trò chức nhóm phiên mã TCP 28 1.3.4 Chọn tạo giống đậu gà chống chịu stress bất thuận dựa nhân tố phiên mã 32 iv Chương II VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 40 2.1 Vật liệu nghiên cứu 40 2.1.1 Hạt giống đậu gà Arabidopsis 40 2.1.2 Hóa chất thiết bị nghiên cứu 40 2.2 Thời gian địa điểm nghiên cứu nghiên cứu 41 2.3 Phương pháp nghiên cứu 41 2.3.1 Nhận dạng, xác định vị trí nhiễm sắc thể phân tích trường hợp chép gen gen CaTCP gen đậu gà 42 2.3.2 Xác định đặc tính Protein CaTCPs 44 2.3.3 Phân tích cấu trúc gen CaTCP 45 2.3.4 Dự đoán yếu tố điều hoà Cis 46 2.3.5 Phân tích tượng lặp gen họ gen mã hoá TCP đậu gà 47 2.3.6 Mức độ biểu gen mã hoá CaTCP RT-qPCR phân tích thống kê 49 2.3.7 Tách chiết RNA tổng hợp cDNA, thiết kế mồi sử dụng cho phân tích biểu gen TCP đậu gà 50 2.3.8 Thiết kế cấu trúc vector biểu gen CaTCP07 mơ hình Arabidopsis 52 2.3.9 Đánh giá khả chịu hạn chuyển gen Arabidopsis chứa gen 35S:CaTCP07 53 2.3.10 Thu mẫu tách RNA đánh giá mức độ biểu gen CaTCP07 chuyển gen 55 2.3.11 Đo mức độ rò rỉ ion 56 2.3.12 Đo nhiệt độ bề mặt 56 v 2.3.13 Mức độ biểu số gen liên quan đến mã hoá enzyme chống oxy hoá 56 2.3.14 Phương pháp phân tích thống kê 57 Chương III KẾT QUẢ VÀ THẢO LUẬN 58 3.1 Xác định phân bố nhiễm sắc thể chép gen CaTCP 58 3.2 Phân tích cấu trúc họ gen CaTCP đậu gà 61 3.3 Dự đốn yếu tố điều hồ Cis 65 3.4 Biểu gen CaTCP quan khác giai đoạn phát triển 66 3.5 Mức độ biểu gen CaTCP rễ đậu gà điều kiện khử nước xử lý ABA 69 3.6 Mức độ biểu gen mã hoá TCP hai giống đậu gà ILC482 Hashem 73 3.6.1 Mức độ biểu gen CaTCP rễ giống ILC482 Hashem điều kiện xử lý hạn 73 3.6.2 Mức độ biểu gen CaTCP rễ giống ILC482 Hashem điều kiện xử lý ABA 79 3.7 Kết thiết kế vector biểu gen CaTCP07 mô hình Arabidopsis 85 3.8 Sàng lọc sơ lựa chọn dòng Arabidopsis chuyển gen CaTCP07 87 3.9 Mức độ biểu gen CaTCP07 Arabidopsis chuyển gen 91 vi 3.10 Khả chịu hạn Arabidopsis chuyển gen 35S:CaTCP07 so với đối chứng WT 92 3.11 Khả bảo toàn tế bào tốc độ thoát nước bề mặt chuyển gen 95 3.12 Sự biểu gen CaTCP07 giúp Arabidopsis chuyển gen phản ứng lại q trình oxy hố điều kiện hạn hán 96 KẾT LUẬN VÀ KIẾN NGHỊ 99 Kết luận 99 Kiến nghị 99 CƠNG TRÌNH ĐÃ CƠNG BỐ LIÊN QUAN ĐẾN LUẬN ÁN 101 TÀI LIỆU THAM KHẢO 102 PHỤ LỤC 129 vii DANH MỤC CHỮ VIẾT TẮT Chữ viết tắt Thuật ngữ tiếng anh Thuật ngữ tiếng việt ABA Abscisic acid Axit abscisic AFLP Amplified fragment length Đa hình chiều dài đoạn polymorphism khuếch đại APETALA2/Ethylene response Các yếu tố liên kết đáp ứng element binding factors với ethylene APX Ascorbate peroxidase Ascorbate peroxidase bHLH basic Helix – Loop – Helix Vùng Helix-Loop- AP2/ERF Helix bZIP bZIP transcription factor Yếu tố phiên mã bZIP CAT Catalase Catalaza cDNA complementary DNA ADN bổ sung CDS Coding sequence Trình tự mã hóa CSD1 Copper/Zinc superoxide Copper/Zinc superoxide dismutase dismutase Chickpea transcriptome Cơ sở liệu hệ phiên mã database đậu gà Cycloidea Yếu tố điều hòa phát CTDB CYC triển tính đối xứng hoa DNA Deoxinucleic acid Axit deoxinucleic DREB Dehydration responsive Protein liên kết với yếu tố element-binding protein đáp ứng nước Ethylene Responsive Factor Yếu tố phản ứng với ERF Ethylene FB Flower bud stages Giai đoạn nụ hoa FL Flower stages Giai đoạn hoa viii gDNA genomic DNA DNA nhiễm sắc thể GM Germination medium Môi trường nảy mầm GS germinating seedlings Hạt nảy mầm HSP90 heat shock protein 90 Protein sốc nhiệt 90 ITS Internal transcribed spacer Vùng mã hóa đoạn RNA khơng đóng vai trị chức vùng mã hóa RNA ribosome nhân kDa Kilodalton Đơn vị khối lượng nguyên tử L1/L2 Line1/Line2 Dòng1/dòng2 MAS Marker-assisted selection Chọn giống nhờ thị phân từ liên kết với tính trạng mục tiêu mRNA Messenger RNA RNA thông tin mW Molecule Weight Trọng lượng phân tử MYB MYB transcription factor Yếu tố phiên mã MYB NAC NAM (no apical meristem), Yếu tố phiên mã NAC ATAF1/2, CUC2 (cup-shaped cotyledon) NF-Y Nuclear transcription factor-Y Nhân tố phiên mã Y NGS Next-Generation Sequencing Giải trình tự gen hệ PCF1/2 Proliferating Cell Nuclear Nhân tố kháng nguyên nhân Antigen Factor1/2 tế bào tăng sinh 1/2 PCR Polymerase chain reaction Phản ứng chuỗi polymerase qRT-PCR Quantitative real-time PCR PCR định lượng QTL Quantitative trait locus Locus tính trạng số lượng RD29A Responsive to desiccation 29A Đáp ứng với nước 29A 130 TCAGATATTCAAATGCAGCAGCATTTTCCATTCATGTCTGAACATCTTATGCAGCAACAA GCTGTGGTTACTTCATCATCATCTTCACATCAAGCAAGTGGGAATAATGATCATCATTAC AATCTTAACTTCGCTATATCGTCTTCGGGCCTTGTTGGTTACAATAGGGGGACCCTTCAG TCCAATTCACAGTCTCTTTTGCATCAGTTGCAGAGGTTTTCACCTATGGATGGATCATCA ACAACATCATCATCCAATAATCTACCTTTCTTCATGGGAGCTACTGTTACTCCTGCTGTT CCTTCAACAATGGACAACAACCATCAACACCTTCCATTTTCACCTGTCTTTGATGCTCGT TTGCAGCTTTGCTACACTGATGGAAGCCGCCATTCAGATCAAAAGGGAAAATGCAATAAG AACTGA >CaTCP02 ATGGGAATGAAGAGCACTGGGGGAGAAATAGTTCAAGTTCAAGGAGGCCACATTGTTAGA TCAACAGGTAGAAAAGATAGACACAGTAAAGTCTACACCGCGAAAGGACCTCGCGATCGA CGAGTTAGATTATCAGCACATACAGCAATTCAATTCTACGATGTTCAAGATCGTCTCGGC TACGACAGACCGAGTAAAGCTGTTGATTGGCTGATCAAAAAAGCTAAATCAGCTATTGAC AAACTTGCTGAGCTTCCACCATGGAATCCAATTCCTAATAATGTCACAGAAGAGTTACAA CAGGAAGAAAATAATGCAGGAACAAGGGATATGGTTATTGCAGCAGAAAATTCAGAATCT TCTGGTTACAATTTTCAGCTACAAGACACAGAAAATCACCATAACCATTCAGCTTTCATT CCTTCAAATATTGATACTGATGCAATAGCTTTTTTTCCAACAAGTTCTGCTGCATCATCC ATAAACTTTCAAAGCTACCCTTCTGATATAATCTCAAGAACAAACAACTCTATACAGGAT CTTGGTCTTTCTCTTCATTCATTCCAAGACCATGGAATGATGCATGGACAATCTCAAGAA GGTGGTAATCATCAAACAGGTTCAAATGACCATCATCAAACACTTTTCACTGATTCAAAT CCTGTTGGATTTGAGGCTAATTATCATAGGATTGTGAATTGGAACAATGATGATCACAGA ACTGGATTCATGGTTAATTCAGCTCCATTTCTTAGCCAAGGTTCTGCATTTTCCCATAGG GGGACCCTTCAGTCCAGTTTTTCGCAATCGCTTCGTCCTTGGAATGATATTCCGATGGAA ACTTCGTCAGTGAATCATAATCAGAAGTCTCAACAGATTCAACAAGCTTCAATCTTTGGT AGCAGATTTCTGTCTGATGCTTTGCCTGGTTTTTGCATTCCAGCTAGAATTCAAGGTGAG GATGAGAGCCATGTTGTTGTTTCGGATAGGCCATCATCTTCTTCTAATTCTCATCATTAA >CaTCP03 ATGGAACTATCAGATTTGCAAAACAACAAGCAGAAGAGAGGCACCCACCAACAACAACAA CAAGAATCATCTTCTTCTCCTTCAAATTCTCATTCTCATTCTCATTCTCATTCTCATCAA CAACACCTTGTGGTCCCTTTTGATGGAAGACCACCCCCTTTCATTATGGGCTCCATCTCC ATCCAACAACCTTCTTCAAACTCTTCTTCTTCTTCTTCCTCCAATTCTTCATCTTCTGCC ATTGACGCTTCTCTCGCCATCGCCACCAGATCTGAACCTCCACTCCTCAATAACACAAAT AACCAATTATCCTCCTCCTCTCCCGCCGTCACCACCGTCGCCAATCCTCCTAAACGATCC ACCAAAGACAGACACACCAAAGTCGACGGCCGCGGCCGTAGAATTCGAATGCCGGCGACA TGTGCTGCCAGGGTTTTTCAGTTGACCAGAGAATTAGGCCACAAATCCGACGGCGAAACA ATCGAGTGGCTTCTCCAGCAAGCAGAACCAGCGATCATCGCCGCCACTGGCACCGGAACG ATCCCAGCTAATTTCTCCACTCTGAACGTCTCACTCAGAAGCAGTGGCTCCACGCTGTCG GCTCCGCCGTCGAAATCGGCTCCTCACAGTTTTCACGGCGCGCTTGCACTGACTCGTCAT CCTTACGAGGAAGCCGCGTACCAACACGCGCCTCTTCTAGGGTTTCATCAACACCACCAA CAACTGATGAGTGCTGATCAAATCGGTGAAGCTTTAAATCCAAACGAGAATTACATGAGG AAACGTTATAGAGAAGATCTTTTCAAAGACGATAATAACAACAACGAAAGTGGTGGTGAT GGTTCTTCTTCTTCTCCAAAAGCTTTCAAAACAAATCATCAACAACAAGATACTATGGGT GAAGGTGAAGGTGGAGGCGGCGGCGGCGGTGGTGGTGGTAGTGGAGCTTCTTCTTCATCT TCTTCTAATAGTTCAATTTCTGGACTTCTTCGACCGTCGAATATGATACCGGCGACAGCC ATGTGGGCCACAGCGGGTAGTGGAAACACAATTTGGATGGTACCGGTGAGTGGCGGCGGT GGTGGTGCTGCAACTTCGAATTCAGAGACACAAACACAGAACATGTGGCCTTTTGGTCCT TCTCCATCTCAAAATAGTCAAACTCAAGGGAACAACGCCGGAACTCCGTCTCCGTTACAT TTTATGCCGAGGTTTAATCTTCCGGTATCAGGTGGTGGTGGTGGTGGTGGTGTTGAGTTT 131 CAGGGTGGACGTGGTGGTTTACAATTGGGTTCAGTGTATCAACCTTCACAGCATATTGGA TTGGCAGTTTCTGATTCAAATTTGGGTATGTTGGCTGCTCTTAATGCTTACACAAGAGCT GCAGCTGGTGCTACTGATTTCAATGTTAATGTTAATTCTGATAATCATCATCACCATCAC CATCAACATCAACATCAACATCATTCTATTTCATTGGATTCACAATCTCATTCTCATTCT CATTCTCATTCTCATCCTCAACCTACTGAAAGTGAAGAAGATGCTCCTGATACCTCTCAA TGA >CaTCP04 ATGAGTGGAGAGATAGTGGAGGTTGACGGTGGTCACATTATACGGTCAAGAGGTAGAAAA GACCGTCACAGTAAAGTATGCACCGCCAAAGGCCCAAGAGACCGACGGGTCCGTCTTTCG GCCCACACCGCTATTCAATTCTACGATGTACAAGATCGATTGGGCTATGACCGGCCCAGT AAAGCCCTTGATTGGCTCATTAATAAAGCCAAGCCCGCCATCGATCAACTCGCTCAACTT CCTCCGTGGAAACCTACTCTCCTCACTAAACAACAGCAAAACGACGACGTTCCACAAAAC CAAAACGAACACGAGTTTCACTTTCTTCAGAACTTCAATAGCAACAACAACAATTTTCTT CCGTTTGATACTGAAACGACGTCGTCTTCGCCGATTCACTTTCAAAGCTATAGTACTCAT GATGACTTGAGGCTCTCGCTTCAACAACATCAGAACCAGCACGTGCATCCGCACGTGCTT TTCGCCGGAAACCTTGATGGAATGGTAGCATGGAATTCCGGTGCTACTATTGATACTGGT AGTAATAATAATAATAATAATCATCATCATGATGATGTTAATTCCGGTGGAGGTTTTGTG TTTCATGCAGCTACACCGTCGCCGGCGGTTTTTCCGGCGGTGATGTATGGTCAGAATCAG TATCTTTCACAGAGGGGACCCCTTCAGTCCAGTAACACTTCTTCAGTTCGTGCTTGGATA GATGCGCCGACGTTTGTTGCCGCTGATCACCGACGTGGTCATGCGGTTGCTATGTCGGGT ACCGGATTTGTTTCTGACAGCTTTTCCGGTTTCCGCGTGCCGGCACGAATTGGGGGTGAT GAGGAGGAGGAGCATGATGGTTTATCTGACAGACCGTCCTCTGCTTCATCCGATTCTCGC CGTTGA >CaTCP05 ATGATTAACATTCAAAAAGAAGAAGATTTTCCATTGAAAGCAAAGGCTTCAACTATCTCA TCATCATCATCATCATCACAATGGCTACAACTAAAAGATCATCCAAGAATTGTAAGGGTA TCAAGAGCCTTTGGGGGAAAAGATAGACATAGCAAAGTTTGCACAATCAAAGGACTAAGA GATAGAAGGGTAAGGCTTTCAATCCAAACAGCAATTCACCTCTATGATCTTCAAGATAGG TTAGGGCTAAATCAACCTAGCAAAGTTGTTGATTGGTTACTAAATGTAGCAAAAGATGAA ATTGATGAGCTTCCACCACTTCAAATACCTCAAGAAAACTTCACCCTTGGTTATCCATCT TTTAATGATGATAACACTTCTATTTGGAAGTCAAACCCTAAACAAGTTTTAGGTGAAATG GTTAATCATAAAGAAAATTGGATAAATAAAAGTGAAGAGGATAAAGTGGAGAATAATGAT TTTCATGATGCACAACATGTTTTGACCAATCAACCTTCTTTTTTGGGATTGTTGAACAAT TCTATGCCACTTGCTTATCATCAATTACAGCCTTCAATTAGAGATATACATTATGGGATA AATGTTGTACCTTTTCCATCATCATTGTCTTTGTCAACACAACAATATTTCCCTTCACAT GTTGTTGGAGCAATGGATATGGATCCAAGACAAATCAATTACAACTATGATCAAATGATG AGTTCAAGTTCTCAAAACAAATTCTCTCAATCCAACTCAACAATTCATCATGAGGGTGGA TCTTAA >CaTCP06 ATGGAATTGGAAGCTGATCATCATCAAAATAATGGAATCCGAAGCAGACCAAATTTTCCT CTTCAACTTTTAGAGAAAAAAGACATCATCGATGATATCTCCGAACAACCTTGTTCAACC ACCGCAAACGACGGCTGTTCCGCCATCGTTACATCAAACAACACGGCCGACCAGCAGAAA AAGCCACCGCCGAAGCGCGCCTCGACGAAAGACAGGCACACGAAAGTCGACGGACGAGGT CGTCGGATCCGTATGCCGGCAGCCTGCGCCGCCAGAGTTTTCCAGCTGACACGTGAGCTC GGACACAAGTCCGACGGCGAGACGATTGAGTGGCTGCTTCAACAAGCTGAGCCAGCCGTC ATCGCCGCCACAGGCACCGGCACAATTCCGGCGAACTTCACCTCACTTAATATCTCTCTT AGAAGCTCAGGATCCACCATGTCGGCGCCGTCGCACTATTTTCGAGGTAACTACTTCAAC CCTAGCTCGTTCTCATCAGCTGCCGCGGCGGCGGCGCAGGTTAGAAGCCGAGCCGAATGG 132 GAAAGAACCATTAATATAAACAATAACATAAATATGAACGTTGTAGCTGAAGATTCAAGA AGAACTAATAGCACAAACATGTTGGAGAATCATAATCCTTCTTCATTATCAGCAATTTTG AATTTCAACAACGTGCAAAATGCTATTCTACAAGCAAAACAAGAGCTCCGGGAAGAAAAC GCCGCCGCAGGCGGCGGCGGTGGTTTCGAATCCGTGGCTTCGGATTCGGATGGAAACATA GGGAGAAAAAGAAGATCGGAGCAAGAATTTTTATCACAAAATATGGGAAGCTATTTGTTA CAATCAAATGTTGGGTCACTTCCTTCAAGTCATGCTTCTAATACGGCAGCGTTTTGGATG GTAACTGGTCATGGCAACCAAGGTGTTAATGTTGGTGGTGGTGGTAATGGAAATGAACCT ATTTGGGCTATTCCTTCTGTTGGTAATAGTGGTAATTTGTATAGAGGAGCTATGTCTTCT CCTAATGGAATTCATTTCATGAATTTTGGTTCTCCAATGTCTCTTATGCCTGGAAGTCAA TTAGGTTCTGGCATCGTCGGTGTTGGTGGTGGTAATGGTGGTTTTTTGGCTGAAAGCAAT TTGAATATGCTTTCAGCTATGAATGCTTATAGGCAGATTAATTCGACGAATTGTGTTTCA GATTCGTCGGGACAACATCATGGAGGTGGTGGAAGTGGAAGAGACGATGGACATGATTCA ACTAGTCAACATTCATGA >CaTCP07 ATGGAAGGGAATCATACCCACGACGATGATTTCAGCGAAGAAAACGAAACACAAAGTACA TCGGAGACAACAGAACCGCAAACGTTGAATACGCCGGAACCAACTTCGGTAATGAAAGAA GAGCTAACGGAAACGGAACGTCATGGCATCGACGAGTCACTTTCTGTTGGTGTTCTACCT ATGCCGATGCCATTAACAGTATCGGCGGCGAAGAAACCGTCGAAAGACCGTCATACGAAG GTGGAAGGTCGTGGCCGGAGAATCCGAATGCCGGCAACATGTGCCGCCAGAATATTCCAA CTAACACGGGAGCTTGGTCACAAATCCGACGGAGAAACAATACGGTGGTTGCTAGAACAA GCTGAACCTTCCATCATAGAAGCAACTGGCACCGGAACAGTTCCTGCCATCGCCGTTTCA GTCGGCGGCACGTTAAAGATTCCAACTTCATCACCGGCGAGACCAGACGGTGAAGAAGCT CCAAAGAAACGAAGGAGAAGAGCATCAAATAGTGAATTTATTGATGTGAATGAACAAGTT TCAATGTGTTCAGGGTTAGCACCAATTGCACAAACTGCATACGGCGGTGCTGCCGGTGGA AGTGGTGGTGGAATAGTCCCTTTATGGCCTGTGGTAAGTAGCGGTGGTTCAAATGCAACA GGACCATTTTTTATGTTCCCTAATTCAACTACGAATCAGCCACAATTTTGGGCTATTCCT GCGGCACCGTTTTTTAATGTTCAAAATAGACCCATTTCAGATTTTGTTTCTGCTATGCAT ATGCAACAACCTTTGAATGTTGCTTCGGCTTTTGGAAATACTAATTGTTCGTCTACTTTG GCTCCTAGTTTGAGCTCAGCAAATACTACTACAACTACTTGTTCTTCTGCTGCTACTTCT TCATCTTCATCTCAGATGCTAAGGGATTTCTCTTTGGAAATTTATGATAAGAAAGAGCTT CAATTTATGGATTCTTCTGCTTCAAATTTAGAACCACCCCCTACTTCAAATTAA >CaTCP08 ATGACTTCAGAACTAGTTATCCACACATTCTCTCAACCACAAACTAATAAACCCTCTACC GCCACCGCTTCCGATCCCAATTCACAAATTTCAACCGTCAAAAGAACTTCCACCACCACC GCCTCTCGATCCACCAAAGACCGTCACACCAAAGTTAACGGCCGCGGTCGTCGTGTTCGT ATGCCGCCTCTCTGCGCCGCCAGAATATTCCAGCTTACGCGTGAACTCGGCCACCGTTCC GACGGCGAGACTATCGAGTGGCTTCTTAGACATGCTGAGCCGTCTATTATCGCTGCCACC GGGACTGGAACTGTCCCCGCCGCTCCAGTCTCCACCGTGTCACCCGTCTCATCCTCGTCG GTTCCCTCCGTTTCTTGCCATGTCCAGCCTGTTTCGTCGGTTGGTCCAGGCGGTCAGGGA ATGTTTGCTGCGGCTATGTCGCAACCACAGCCGAGCTGTCGTCTTGACCTATGTCAGCCG CCGGGAATGGAGTATGCTGCGGCGAATGGTTACCGTCACATGCCGTTTACGGCGTTGTTG TTGCAACCGACGACGGCGGAGGAGAATCAGCAGGAAGAAGCTCTGAGCGAACAATGA >CaTCP09 ATGTCTAACTGCAAGGAAACAACTAACGCCGTAACCGTCACTAACGGCACAACAACAGAC TCTCAAAAGACCATCGCCGTTAAAAAACCGCCGTCAAAAGACCGTCACAGCAAAGTAGAC GGACGTGGAAGAAGAATACGTATGCCAATTATTTGCGCCGCACGTGTTTTCCAGTTAACA CGTGAGCTCGGTCACAAATCCGACGGTCAAACCATCGAATGGCTTCTCCGACAGGCGGAA CCCTCCATCATCGCTGCCACCGGCACCGGAACTACTCCCGCTTCATTTTCATCTCTCTCT 133 GAACACAAACCTTTTCACCCTCCTTCTCCTTTCATTCTCGGAAAACGGTTACGTTCTGAA CAAGATGACGCCGTTTCCGTTGCTGCACCTACCGCTCCACCGGCTCTTTGGGCGCTTCCG TCAAGGCCTGACTTCGCTCAACTTTGGAGCTTCGCCGCCGCCGCAGCACAACCTCCGTCG GAAGTTCTTTCCATTTCTCCGCAACAGCAACAGCATAATTCGTTGTTTCATCATCAACAG CAGCAGCAACAACAACATCAAGCTTCCATGGGAGAAGCTTCGGCGGCGAGGTTAGGGAAT TACCTTCCTGGACATCTTAATTTGCTCGCTTCATTGTCCGGTAACGGAAATTCCGGTCGG GGAGACGACGAACATCATTGA >CaTCP10 ATGTCTAACTGCAAGGAAACAACTAACGCCGTAACTATCACTAACGGCGCAACAACAACA ACAACAGAACCGGAAACTACGCAACGAAACACCATCGCCGTTAAAAAACCACCGTCCAAA GACCGTCACAGCAAAGTAGACGGACGTGGAAGAAGAATACGTATGCCAATAATTTGCGCC GCACGTGTTTTCCAGCTTACACGTGAGCTCGGTCACAAATCCGACGGTCAAACAATCGAG TGGCTTCTCCGGCAGGCGGAACCCTCCATCATCGCCGCCACCGGCACCGGAACCACTCCC GCTTCATTTTCATCTCTCTCTGAACACAAACCTTTTCTCCCTCCTTCTCCTTTCATTCTC GGAAAACGATTACGTTCTGAACAAGACGACGCCGTTGCCGTTGCTGCGCATCCTGCTCCG CCCGCTCTATGGCGGCCTGACTTCGCGCAACTCTGGAGCTTCGCCGCCGCCGCACAACCT CCACCGGAAGTTCTTTCCATTTCTCCGCAACAGCAACAGCACAATTCGTTGTTTCATCAT CAACAACAGCAGCAACAACAACAACATCAAGCTTCCATGGGAGAAGCTTCGGCGGCGAGG TTAGGGAATTACCTTCCTGGACATCTTAATTTGCTGGCTTCATTGTCCGGCGGTAACGGA AATTCCGGCCGGGACGACGATGAGCATCGTTGA >CaTCP11 ATGTTCTCTTCCACATATCATGACTCCAACCCTTTACCTAATTTCCCTTCATCTTCGTCT TCTTCTTACCCTACCCTTTCTTTTCTCATTGACCCTCAAAATGATTTTCCAAACAACACT CTTCTTGATGATCCACTTTCTCTTCCATTTTTACCCTCAACAACAACAACACATGATGTT CTTCCAATATTCCCTAATGAAACTACTATTAATGCTAATAATTTGGCTACTTCATTGTTT GAACAAGATATTTCAACAAGTACTAATGTTAATAATTGTAATTCCAACTATGGTGTTAGT GTTGTTCCAAATTTTGTCACACAGAAATTACCATCTTCCACCATAGGAAAAAAAGATAGG CACAGCAAGATTCACACATCACAAGGTTTGAGAGACAGAAGGGTTAGATTGTCAAGTGAA ATAGCTAGAAAATTCTTTGATCTTCAAGATATGTTAGAGTTTGACAAACCAAGTAACACA CTTGAGTGGCTTTTCACAAAATCTGAAAGTGCAATCAAAGAACTAGCTAGAACAAAAAAC ATTGGTTGTTGCAACAATAACAACCCTTTTGAATATGATACAAATAACAACAACAACCAC AAAAACTCATCATTTTCAGGTGTTGGTGATAATAAACAAGGGAGGAATAAGTTGAAGTGG ACACAACAAAAAGAAGAAGCTTTTGTTGTTCAAACAAAAAAGGAGTCAAGAGAAAGAGCT AGAGCAAGGGCAAGAGAAAGGACTTGTTACAAGATGTGTAGCACTAGTACTAATGGATCT TCAACAAATCATCATCATCATAATCATAATCAAATGCTTCAACAATTGATGATGTCATCA TCATCACCTATTGATCATCAAAGTAGTACTGAAAATGATGAAGCTTATGCAAGATGGCGT CAGCACCTTGTTCAACTTCAAACCTACCACCCTTCTACTTATTCTCATCATAACCTACTT GATGAGAGTGAAATACCTAGAGATCATTATGGTTTTAATGTCATTGAGGAATCTATTATG ATCAAAAGGAACATGATGTCTTCTACTTCTTCTCATCATCACCATCAACAAAATCTCGCA ACTATCCCTAAAGAACCAACTTTTAACAACAACAATATTATTGATTACCCTAATTTATTT CCTTATTCAACTACCAATTGGGAGACTAATGGAGGTTGCTCCAACTTTTATGGAATAGCC ACCATGAATCTATCTACATGTTTCATGAATCAGTGA >CaTCP12 ATGATTAAGAATTCAAAAGAAGCATTGAAGCAAGAGGGTCATTCAAACTCAAACAATGAT GAAAAAGAAAAGGCTTCATCATCTAACTCCTTAGCATCACAATGGTCAAGATTGAAGGAT CCAAGGATTGTTAGAGTATCAAGAGCCTTTGGTGGAAAAGATAGACATAGCAAAGTTTGC ACAATAAGAGGATTAAGAGATAGAAGAGTAAGGCTTTCAGTTCCAACAGCTATTCAACTT TATGATCTTCAAGATAGGTTAGGGCTTAATCAACCTAGTAAAGTTGTTGATTGGTTGCTT 134 AATGTAGCTAAGCATGAAATTGATGAACTTCCACCACTTCCAAATTTTCCATCTCATGGA AATTTCAACTTTGGTTTTCCATCTATTAATATTGAAGCCAACACCAACACCTCTCAACTT AATCAACAACTATTGAATATCAATAGGAATATTCCATGGGAAGGTTCAACTTCAAGCCAA AATTCTATGTGGAAATTGAAGCCTAAAGAAGTTACACAAGAAATGGTTATTGATAATAAA GCAAATTATTGGATGAATAGAATTGAAGAAGATGATGATAATAATAACAAGCATGGAAGC AATGAAGGTAATAATTCTAGTGACCATGTTTTGTCAAGTAATAATAACAATTTGTTATTA CAAAGAACCAATCATCAAGCTTCATTTCTTGGCTTGTTAAATACTATGCCATCAATGGGT AATTACCAATGGGGAGGTAATTCCAATGTTGATGTTAATTCTCAATTGGGAAACCATATT GGTTTTACAAACCAAACAAATGTTAATGTTGTTACATCTTTGCCACCAATGTTGTCTTTA TCAACTGGAAATTCTCAAATGATGCCATATTTCCCTTCTCATCATCATGTTAATGCAATG GAGATGGATAATCATCATCATCAAAGACAAATCAACCATCATAACTATCAAATGTTGAGT TCAAGTTCTCAAAATCCATTGAATAATTCTCTCAACAATCCATCCTTCAATTTGGCTATG ATGATTCCTAAAGAAAATCCATCCAATAAAGATCAAGATTTCACTCCTAAGTGA >CaTCP13 ATGGAGGAGGATGAGATTCAAGTTCAATCACAAAACCAGTCATGTAAATTTCCAAGAGTT GGAAACAACAGAAATGATCAAGAAGAAGATTTAGAAGATGGATCAATGAGAAGAGTAGGA GGATGTGATGGAAACACAAACAGATTAAATAATTGGCATCATTCATCAAGAATCATAAGG GTTTCAAGAGCTTCTGGTGGAAAAGATAGACACAGCAAAGTAATGACTTCAAAAGGTTTA AGAGACAGAAGGGTTAGACTTTCTGTAACAACAGCTATTCAATTCTATGATCTTCAAGAT AGGTTGGGTTATGACCAACCAAGTAAAGCTGTTGAATGGTTGATTAAATCAGCTTCTGAT GCTATTTCTGAATTACCTTCACTCAATAACACATTCCCTAATGATGATGATACTAATCAA CTACTACAACCAAGTAGTGAACAACAACAAGGTCTTGATTCAGCTGATGCAGAAATGATT GAGAATGGTGAAAACAACAACTATCATCAGAATCAAAACAACCAGAACAATCTTTGTTTG TCTAAATCTGGTTGTAGTAGTACTTCTGAAACAAGTAAAGGTTCTGGTTTGTCTTTGTCA AGGTGTGATATTCGTGTGAGTCGTGTTAAGGCTAGAGCAAGAGCAAGAGAAAGAACAGCT AAAGAAAAGGAAAAAGAGAACAATGAGTGTTCTCATCATCATCAACAACAACAACAACAT CAAATTTCTCAAACAGCTTCATTCACTGAACTACTTACTGGTGGAATCAACAATGCAGTT GCAAATAGTCCAAGAGGGTCATCAATTGATGAAGCTGATCTTTTCAACAAAGCAAGACAA CAACAATGGAATTCAGCAGCACCAATGGATCATTACTTTAATTCAGTACTTCTTGGACCT TCTTCTTCTTCATCATCAAGAACACATCATCAATCTTTGTCACAAATCCAATTAGGACAT TCACTTCATGAACAACATCATCAATCAATGTCATTCAGCAATAATAGTGGTGAAAATCAG AATCATCAGATGCAGCAGCAGCAGCAGCAGCATCAATTTTCATTCATACCAGATAATAAT GATTACAATCTGAACTTTAAAATATCTTCTTCTTTGGGCCTTGCTGGTTACAATAGGGGG ACCCTTCAGTCCAATTCTCTGTCTCATCTTCAGAGGTTTTCAGTTTCACCTATAGATCAT GGATCCAATGTAGTAGTACCTTTTTTCATGGGTGGAGGAGGAGGAGGAGGTGCTTCTTCT GCTACTACTGCTTCACCTTCACCTTCAGATGCTACTCCTACAATGGAGAATAATCATCAG ATTCATCATCATCATCATCAGTTTTCATCTGTTTTTGATGGTAGCCGTTTGCAGCTATAT TCAGATCACAAAGGGAAACCCAAAAACTGA >CaTCP14 ATGAGGAGGAGTAGTAGAGGAGGAGAGATAGTTGAAGTAGAAGGAGGACACATAGTTAGA TCAACAGGTAAAAAAGATAGACACAGCAAGGTGTATACATCCAAAGGACCCCGTGATCGA AGGGTTCGTCTCTCAGCACATACAGCCATTGAGTTCTATGATGTTCAAGACCGCCTTGGC TATGACAGACCAAGCAAAGCAGTTGATTGGCTCATCAAGAAAGCTAAGCATTCTATTGAC AAGCTTGCTGACCTTCCTCCTTGGCATCCTTCCTCTGATAATCAAACTCAACAACAACAG AATGTTGCTGCAGGATCAACTAGCTTGGTTATTGGAGAACAACAATCTCAATCTAATAGT GGATATAATTTTCATCTCTTAAGACACTTTACTGATAATCAATGTGACAACAATAATTCA TCTTTCATTTCACAACATGCTGTTGAAACTGATCCTATACCTTTTTTTCCAACTACTTCT TCTACTTCTCAACCTATCAATTTTCAAACCTACCCTCATCATCATGGAATAATCTCTACA 135 AATAACATTCATTCAACACAAGATCTTGGTCTATCTCTTCATTCATTCCAAGATCATAAT GGTTTGATTAATCCTTGGCAATCACAGCAACAGGATTGTCAGAACCATGCAACTTCAAAC CAAAACCAAACACTTTTTGCTGGATTTGAGAACCAATATCAGAGAATTGTTAACTGGAAC AGTGAAACTACCACTGATTATGTAAAGAAAGTTGGCTTCATGGTAAACTCACAACAATTT CTTCCCCATGGTTCAACTTCAGCTTCAGAATACTCACCAAATGAGACCCTTCAGTCGAAC TTTTCGCCTTCGGTTCGATCATGGAATGACATTCCAATGGCTTCTTCAGAGCATCACAGG ACAGAACAAGTTCATCAAGCTTCAATATTTGGAAGCAGGTTTGTTTGTGATGGATTAGCA GGGTTCTGTGTTCCTGATACAGTTCAAGGTGATGAAGAGGGTCATGGAGTTTCTTCCAAT AGGCCTTCTTCTGCTTCTCCTACTTCTCATCACCATTGA >CaTCP15 ATGTATTCATCAAACAGTTCCCTCAATGGAAATAACAATAACCTAACAGGAACATCATTT TGTTCATCTACACCTTTTACTTTTGAAAGCAACTCATCATCATCAACTTCAAAACATCAT CAAAACACTTGTTTTCCACCCTCACCTTTCTCTTTCATTCAATTCCCTTATGACACTTTT GAAGACAATAATCAAATCTTCCTCCTCCAAGAACAAGATGATCACATTCAGATGAATGTT AAAAACAAAGAAGAAGAAGAAGATAAAGGAAAAACAGTACTAGTAGTGGTTGATGAGCAT AAGAAGAATGTTCAAAGAAAAAGATCCAGCAAGAGAGATCGACACAGTAAGATCAAAACG GCAAAAGGATTAAGAGATCGGAGAATGAGATTGTCTCTTGATGTAGCAAAGAAGTTTTTT GGTCTTCAAGATATGTTAGGATTTGAGAAAGCAAGCAAAACAGTTGAATGGTTACTCAAC AAATCAAAACTTGAAATCAAACAGCTTGCAAGAGAAAAGAATCTTCAGAATGATTCTTCA TCAACAACTTCAGAATGTGAAGAAGGTGTTTCAAGTTTGGATAATAATGATCATCAACAA CAACAAAAAATCATGCTTGTAAAGAGATCATCATCAACAAACAAAGTTTCTAGAAAGAGT GCATTCAATTGTATTGGAAGGGAAAAAGCAAGAGAAAGAGCAAGAGAAAGAACAAGACAA AAGTTGAATGCTAGAATAAGAGATGTTAATATTGTTGATCAATCAAATTCAAAGCAATGT GTTAATAATAATAATAATAATAATAATTGGAACCCTTTTGAAGAATGTGCAGTGAATAAT AGTTCCTTTGATGTGAAGATGATTAATCATGAAGCAAAGGAACATTCAGAATTAGAGGAT GAAGAAAATTCCTTGTTCATTAATATGACCAAATGGAGTCCAACTTTCATGTTCAATAAC TCATCAATCCTACAACAACATCATCAATATGCACAATTTCAATGTTTGGAAAAACCTTGG GAGGACTACAACAACACCATCTAA >CaTCP16 ATGGATGGAGGTGATGATCTCCACCACCATCACCAACACCAACACCATCACCACCACCAT CACCAACAACAGCGACAAAACTTCCCATTCCAACTTCTTGAAAAAAATGAAGACCAAGAA GCAGCATCATGTTCAACCTCTTCACCGTACCCATCCTTAGCAATCTCACCAACAGAACCA ACCACATCAAATTCAAACCGATCCAACCAACTAGTCTCCGCACCAACAACCTCCTCCGAA CCAGGAAACAAAAAACCACCACCAAAACGAACTTCCACCAAAGACCGTCACACCAAAGTA GAAGGCCGCGGCCGCCGTATCCGAATGCCGGCTCTCTGCGCCGCTAGAGTTTTCCAACTA ACACGCGAACTAGGCCACAAATCCGACGGAGAAACCATAGAGTGGCTCCTCCAACAAGCC GAACCAGCTGTCATAGCCGCCACCGGAACCGGTACAATTCCCGCTAACTTCACCTCACTC AACATCTCGCTTCGTAGCTCTGGTTCATCCATGTCGGTGCCTTCTCAGCTAAGATCTTCA TATTTCAACCCGAATTTCTCACTTCAACAACGCAGATCACTTTTCCCTGGAATCGGTTTA TCATCTTCCGATAACAACAGCAACAACAACACTTCAACGCTCTTAAATTTCCAAACGAAT AACCTAACAACAAACATGTTGCAGGCTAAACAGGAGCTTAGAGACGGAGGAGGAGGAGGA GGCGGCGGCGCACCGTCTTCTTCAACAACATTAGATTTATCAGAAACAAGTGGAGAAGAA AACATGGGAAGAAAAAGAAGACCTAGCGAACAAGATTTAACATCATCAACAACATCACAA CAACAAATGGGGAGTTATCTTCTACAGTCAAGTGCAGGTTCAATTCCGGCAAGTCATACT CAGATTCCAGCGAATATATGGATGGTAGCAAATTCAAATAACCACCAAGTAATGAGTGGT GATCCAATTTGGACATTTCCATCTGTGAATAACAGTGCTGCCGCAGCTGCTGCTTTGTAT AGAGGAGCGGTTTCAACAAGTGGATTACATTTTATGAATTTTCCACCACCTATGGCTTTA TTGCCTGGTCAGCAATTAAGTAATTCATCTCTTGGTGGTAATAGTAGTGGTGGAGGAGGG 136 GGTGGGAATATCAATATGAATATGAGTATGAATATGAATATGAATGAAGGACATTTGAGT ATGCTTGCTGGTCTTAGTCCTTATCGTCCAGTTTCTCATCATGAATCTCATCAACAAGGA AGTGGATCACAATCACAACGTGGTGGTAGCCATGATCGACATGATAACACAAGTCACCAC TCCTAG >CaTCP17 ATGGGAGAAACAACACAAGACAACAACAACACAGGAGGAGCAGGAACAGCAACAACCACG TCGAAGAGAATCGGAGGAGGAGAAATCGTGGAAGTACAAGGAGGTCACATTGTCCGTTCA ACAGGACGGAAGGACCGTCACAGCAAAGTTTGCACAGCGAAAGGACCCCGGGACCGCCGA GTACGACTATCGGCCCACACCGCTATCCAATTCTACGACGTCCAGGACCGTTTGGGCTAC GACCGACCCAGTAAGGCAGTTGATTGGCTCATCAAAAAAGCCAAAGCTTCAATTGACGAA CTCGCTCAGCTTCCCGCATGGAACCCCACCGCCACGACGCCGCCATCTTCAACACCACAA CAACATCATCAACATACAGATGAAATGTTCCACCGGCAATCCCCGGTGCACGATGACCCA GTGACCGGATCCACTAGCCGTAGAGCAGCGGCATTGGAGCGAAGAATACCACAACAATTT TCTTCGCAACAATTAGAATTAGAAGATAATAATAATAATAACAATAATAATAGCGGGAAA CATAAATACAGCAATGCTTCTACTTCCGGTTTTCTTCAAGGGTCATTGGACACTGACATT GCGGATACTATTAAATCGTTTTTTCCAGTGGATACGGCTACGACGTCGTTTCACAGTTAC CCACCGCCGCCATCGGATTTGCTTTCTCGAACGCCAACCACTCAGCAAGATCTGCGTCTC TCGTTGCAATCTTTTCAGGATCCTATTATGCTTAACCATCAGGCACAGAACCATCACGTG CTTTTCGCCGGAGCTTCTTCGCTCGGCTTCGACGGCGGTGGATGGCCTGAGCAGCAGCAT CATGAAGAACATGACCATGGCAGATTGCATAGAATGATGGCTTGGAATGCTAATGCTGTT GATGGCGGTAACAGTGGCCATGGCGGTGGATTTGTCTTCACCGCTCCGACGCCGGCGTTC GGCGGTCACGCTCAGTTTTTTACTCAGAGGGGACCCCTTCAGTCCAGTAACACACCTTCA TTTCGTGCTTGGATAGATCCGTCCATGGCGACGGCGACTATTGCCGATCACCACAACCAT CACTACCTTTCGCCAATGATCCATCAGGCTTCTGTCTCCGGTGGTGGATTCTCCATACCA GCACGAATTCAGGGTGAAGAGGAACACGACGGTGTATCCGAAAAACCGTCCTCTGCTTCC TCTGATTCTCGCCATTGA >CaTCP18 ATGTCTCAAGAAGGTTCTAGAAACATTGATCTCACGCTAGGAGAACCCAAAGAAGAACCA GAAACGGAGGATGGCTCAGCGATAAACGTGATGCCGATGGCGATGCACGTGCCGACGGGG ATATCCATGTCCATGCAAGTAAACAAGGCTTTAATACAAGCCCAAGCCCAAGCCCAAACC CAAGTAATCCAACACCAACAACCGCTTCAAAAGAGAGCCTCCACGAAAGACCGTCACACA AAAGTAGAAGGTAGAGGTCGTCGGATCCGAATGCCTGCCACGTGTGCGGCTCGGATCTTT CAATTGACCCGAGAACTCGGTCATAAATCCGACGGCGAAACAATCCGGTGGCTCCTCGAA CACGCCGAGCCCGCCATTATCGCCGCCACCGGAACCGGCACCGTCCCCGCCATCGCGATG TCCGTTAACGGCACTCTCAAGATCCCAACAACTTCTCCCAATAAAAGCTCCGACCCGGAA CAAAACCAACAACAACTGGAAGATTCACCTGATAAGAAGAAACGTAAACGACCAGTTAAT AGTGCTTACGTGGATATAAACGACAGCACCGTTTCAGTTTCAGCAGGTTTAATAACAACC TCAATTTCAAACGCAAACATAAACACAAACCTAACTACCACTGCTATAATTCCGCAACAA ACTGCAATTCCGTTACCGCAAAATATGGTACCTGTGTGGGCCATACCATCAAACGCAGTC GTTCCAGCGGGAAGTGCTTTTTTCGTGGTTCCAACGAATCAGCCTCAGTTTTTCACGTTA GCAAGACCAATCTCCGCTTTCGTTTCCTCCATGGTAACACCCGTTCAAATTCAACAAACT TCCACGTCATCCAACACGTGTTCGATTTCACCTCCATCAAAATCTCCGGCGAGAGCGTCG GTTATGGCTCCGACCGCCGCCGCCACAACCACCACGCAGATGCTGAGGGATTTTTCGTTG GAGATTTATGATAGGCAAGAGCTTCAGTTATTGTCGCGATCTTCTTCGAAGCATTGA >CaTCP19 ATGATGATAGAAAATTCAAGTGAAAGTTATGAAGCAAAGCAAGAGGGTGGTGATGGAATA ATGAAAAGTGAGAAATTAGCAGGAGCAGGTAGTACCTCAAGACAATGGACATCATTAAGA AATCCAAGAATAGTGAGAGTGTCACGTTCTTTAGGAGGAAAAGACAGACATAGCAAAGTT 137 TGTACAATTAGAGGACTGAGAGACCGTAGGATTAGGTTGTCTGTACCAACAGCTATTCAA TTATATGATCTTCAAGACAGACTTGGTTTAAGCCAACCTAGTAAAGTTGTTGATTGGTTA ATTGAAGCTACTCAATCTGATATTGATAAGTTACCACCACTTGATATTCCTCAATGTTTT ACTTCTCACTTTCATCATCATCAATTACCACCACCAGGTACCTCTTTTTCTCTTGGAGGA TTTTATGATCAAAATAATGGGATGGCAAAGACAAGGTATAATAATTGGGATATAGATTCA TCAGTTGGAAGATTAAAAGGTAAGGAAGCTGAAAATGTGTGTATGGATTCAGAAAAGAGT AAGTTATGGATGAAAAGTACAAATGAACAATTTGGGAGTAATTATTTTTCTAATTCTTCA CCATCAGTACATGGTTTATCAAGCTACCATAATTTGGATCCATCAAGTACTTTATCTTTA TCCCAATTTGGAAACAGTGGTAATAATATATTTCCTTATTACCATCAACAGGTTGAATCT CATCATAATAGTGGCACTGTTATGCAGTTTTCATCTTCATCTTTCACTAATGGTAATTCA CAGTTACTTTTTTGTCCATCATCAGTATCACTTTTTAGTAACAACAACAACTCTCTGGAA ACTACTGATAATCATGCAGCAAGACAACATCTTCATCATCAGATATTGAACTCATCGTCG TCGTCATTGTCGTCATCTTCTTCTTCTTCACATGCACTCATGATGCCTCTCATTCCAACT CTTCATCTTCCAATCAATTCGCCTTTTAGACGCCTTCCGTTACCGTTTAGTTCCAAGCTT CTTGATTCAGATCATGATAATAATAACACCAGAAATGGTTAG >CaTCP20 ATGTTCCCTTTCAATTCAAACCCTTACCCTTCTTTTGTTCCTAATTCATCAGCTTCTTCT TCATCACTTTTTTCTTTTCCTTTTCTCAATCCTGAAAATGCTTCTTCAAGTAACAACAGC AACAACAACACTCTTTTTCTTGATCCATTTTCTATTCCATACATACCAACTCATCATCAT CATCATCCTCATCATCATAACATTACAAATATCCAAGAAACCCTAACCAATTTAGCTGAA AATAATAATAATAGTAATAATAATAGTAATAGTAATAATAATAATAATAATAATAACATT GCAATTCCAATGGCTAAACAAGATCCTTCAACAATTGGTGGTGGTTCTCACTATGGAATT TCTTGTTTCCTTGCAAAGAAACCACCAAAGAAAGATAGACATAGCAAGATTTACACTTCT CAAGGTTTGAGAGATCGAAGGGTGAGGCTTTCGATCGAGATCGCTCGAAAGTTCTTCGAT CTTCAAGACATGTTAGGGTTCGACAAAGCTAGCAACACACTTGATTGGCTTTTCACAAAA TCAAAAAAAGCAATTAAAGATCTAACAAAAAGCAAGCAAAAAAACAACAATGAAGATGGT GATGATGACGATGAAGATGCTAAAAGCTTCACTTCTTCTTCGGATTGTGAAGTTGTTTCG GATATCAAGCAACATTCTTCAAACCTAAAACAAAACCAAGATGGTGATGGTGATTTTTCT AAAGAGAAGAAGTTGAAAAGGGCACAAATCAAAGAATCTTCTTGTATTAGGGCAAATAAG ATGAAGGAATCAAGGGAGAAAGCAAGAGCAAGAGCAAGAGAAAGAACAAGTAACAAGATC TCAAACAACAATAATACATTGAAGATTCATCAATTAGAGTTGAAGAAAAAGTACCCTACA ACAAATGAAAATTTTCAAGCTTTTCACCAATCTAGGTCATCTTTACAAGATCATCAACTT GTGAGTAGTAGTGAAACACCAAGAGATGATTTCAATGTTATTGAGGAATCAATTGTGATT AAGAGGAAGTTGAAGCCATCTTTGATGGTTTCTCATCATCATCATCATCAACATCAAAAT CATGTGATCCCTAAGGAAGCAACAAATTTCAACAATAGTGAGTACCATTCATACCCTAAT TCTTCACCAAATTGGGATGCAAGTAATAATAGTGCTTCTTCCTCTAATCGATCCACCTTT TGTGCTATTGCTAGCATGAATCTTTCTACAGGGCTCCAAATATTTGGAAAATCATGGGAG GAATGCACCAATCCACGTCTACATTAG >CaTCP21 ATGGATCCCAAGAGCTCAAAACAACAATCACAAGAACCAAACAACAACATGGGAGAGAAC AAACCCTCTGATTCAAAAGACTTCCAAATAGTATTATCTGAAACAACAACAAAAGATGAA ACAACAAAAAAACAACAACAACTAGCACCAAAAAGAACATCAAACAAAGACAGACACACA AAAGTTGAAGGAAGAGGAAGAAGAATAAGGATGCCAGCTTTATGTGCAGCTAGAATCTTC CAACTAACAAGAGAATTAGGTCACAAATCAGATGGTGAAACAATCCAATGGCTTCTTCAA CAATCTGAACCATCCATCATAGCAGCCACAGGAACAGGAACAATACCAGCTTCAGCTTTA TCTTCTTCTTCTGTTACAACACAACAACAAGGTAGTTCTTCTTGTTCTTTGTCTTCTGGT TTGCAATTACATCAAAAAATTGATGAATTAGTTGGAAACAGAATAACCAGTTCAAATAGT TGGGGTACTATGGTTGGTGGAAACTTAGGAAGAACAAATATGTGGACCCCACATCATCAT 138 GTTAGTGGATTTGGATTTCAATCATCATCTGCTCATTCTACTACTACTAATGATCATGGT TCAAATTATCTTTCAAAAATTGGGTTTTCTGGATTTGATCATCAAAATATTAATAGTAAT AATAATAATAATAATGGAAATATGGTTGGTGGAATGAGTTTTACATCAATTTTGGGTGGA AATCATCAGCAGATTCCTGGATTGGAATTAGGGTTGTCACAAGATGGACAGCATATTGGG GTTTTGAATCAACAAGCTTTGAGTCAGATTTATCAGCAGATTGGTCAAACTAGGGTTCAT CATCAACAGCAGCAGAATCAGAATCAGAATTCTAGTAAGGATGATTCTCAAGGTTCAGGG CAGTGA >CaTCP22 ATGGCAAGTTCAAGAGAAGGTTATGAAGCAAAGCAAGAGGGAGGTGAAAATAGCAATAAT AATAGTATTGAAAAGATTTCAAAGGCAGCTTCTACTTCAAGACAATGGTCATCAACAGGG TTAAGAAATCCAAGAATTGTGAGAGTATCTCGTACATTTGGTGGAAAAGATAGGCATAGC AAAGTTTGCACTATAAGAGGTTTAAGAGATAGAAGAATTAGGCTTTCAGTTCCAACAGCA ATTCAATTATATGATCTTCAAGACAAACTTGGTTTAGGACAACCAAGTAAAGTAATTGAT TGGTTACTTGAAGCAACAAAATTTGACATTGATAAACTCCCTCCACTTCAATTCCCACAA GGCTTTTCACAATTTCATCATCCACAAACCCTACTTCCTTTTCATGAATCAATTACTAGT GCTTCTCATGAACTTTCACTTGGTCCTTTGTATGATCCTAGTTCATCATTTGTTGGGATT CAAAATCTCATGGCAAGGTCAAAGTTTTGGGAAATGGATTCAAGGGTTAATAATAAAGGG AAAGAAGTTGAAAGAGAGTACTTTGTTGAAAAGGGTAAGTGGATCAAAACAAATGAAGAA GAAAATCATGAAGCTAGTTACAATAATTTTCAACAAACTTCAACTCAAAAGCTTTTTCCT ATGGAGACTCATTCTACTTTACCAAGTTTTCTTAACAATAATGCTATGTCATATAACAAC TACCATTTAGAACCTTCTAATTTGTCTTTATCTCAATTTGGAGGAAGCCATGGATTGTTC CAATCACAACAAGTAGATCCAAATCAAAGTAGTGGAAATGGTTTGCAATTTCCATTTTCT TTTCCACTTCAACAATCTACTTCACAATTATTGTTTGGACCTTCTTCTTCTTCTACTTCA CCATCATCATCATCACTTTTGAGTAATCCTTTTATGACAACAACAAATTCAGTTGAAAAT GATCCAAGAGTACAACAATTCAACCATTTTCAGTTCATAAACTCATCTAGTTCTTCTTCT CAAGCTATGACTCATCCACTCCTTCCATCTTTTCACTCATTCAACTCACCTGCTGTTAGA CCTTTTCCAATGCCATTTAGTTCCAAGCTTCTTGATTTGGATAACAATCACAATGGTAAC CAACATGATAATAAGGGTTGTCGCGGCGATCGTTCTTGA >CaTCP23 ATGTTTCCTTCAACTTATTGCTCTTTAGGTCCTTACCCTTGTTATACTTCTTCATCTTCT TCACATTCATACCCCCCTTTTACTTTTCTTACCCCTGAAAATGCCTCTTCAAACACAAAC AACAACAACAACAACAATATGAACACTTTTCTTCATGATCCAGTTATTACTATTCCTTAC ACACAAACAACACATCACCATGTTCCAATTCCTGAATCCTTAACAAATTGGGCTGTTTCA GATTATGCTACAATGTTGAAACAAGATCTAAGTCATCATCATGGTTCTTCATATGGTTTC ACCAATTTGCTTACAAAAAAACAAGTTAAGAAACCAGCTAAGAAAGATAGACATAGCAAG ATTCACACATCTCAAGGTTTAAGAGATAGAAGGGTAAGATTGTCAATTGAGATAGCTAGA AAGTTCTTTGATCTTCAAGATATGTTAGGGTTTGACAAAGCTAGTAACACACTTGAATGG CTCTTCAACAAATCAAAAGAAGCAATTGAGGAACTAACAATAAGCAAGAACCCTAGTGCT AATGTTGATGATTATGATGCTAATGATGATGTTAATAATGATGATGATAGTTTCTCATCT TCATCATGTGAAGATATGATTGATAATAAAGAGAGAAAAATGAAAAGGGTAGTTAAGGAA ACTTCAAAAATGAAAGATTTAAGAGGAAAGGCAAGAGCAAGAGCTAGGGAAAGNNNNNNN NNNAGAAGATGCGAAGATTTGAAGGAAAAATATGTTGAAAGTGAAAACCCTCAACAAATT CTTCATCAATTGATGCCTAATGATGATGAAAACAACATTTCTAAATCACAACAACCTAGG TTAGTTCATCATTTAGGTGTTGGTGGTGGTGAGTTTCAAAGAGATGATATTTTTAATTTT ATTGAAGAATCTATTGTGATTAAGAGAAAGTTGAAGAAATCTTCAGTTTCTTCTTCTTCT AATTCTCATCATCAACAAAACAATTTGATCTCTAAGGAACCATTAGGTTTTGATAATCAT AACAATGATTGTCATGAGTCTACTATGTCACCAAATTGGGATGCTAATAATGCTGGAAGA TCCAACTTTTCTTCAATATCAAGAATGAATCTATCATCAGGGCTTCAAATCTTTGGAAAA 139 TCATGGGAGGAGTGCAATAGCAGTCCAAACAATCGATATTAG Trình tự amino axit >CaTCP01 MEDEIEAQACKFPRLGNGSSNNKNIGKYPLDDEEQELIIRRKNVREIGRIDDVNVNTRNH FQNHSSRIVRVSKASGGKDRHSKVMTSKGLRDRRVRLSVTTAIQFYDLQDRLGYDQPSKA VEWLINAASDSISELPSLNINTFPQTPTHERQNNNNNLNSLSKSACSSTSETSKGSERAK EKDNESSFAQQQQQQQHHHDVSVNHNHHISQTASFTELLTGGIGSSNVRIPTTTSPNGSV HDSQINNHGDEFNKTRHHQQWSSTVTPIMDYFNSGLMVSNSRTHHHNQSSTSSSSSSGCF QLGQQHSLPVSPFSNSGENNSDIQMQQHFPFMSEHLMQQQAVVTSSSSSHQASGNNDHHY NLNFAISSSGLVGYNRGTLQSNSQSLLHQLQRFSPMDGSSTTSSSNNLPFFMGATVTPAV PSTMDNNHQHLPFSPVFDARLQLCYTDGSRHSDQKGKCNKN* >CaTCP02 MGMKSTGGEIVQVQGGHIVRSTGRKDRHSKVYTAKGPRDRRVRLSAHTAIQFYDVQDRLG YDRPSKAVDWLIKKAKSAIDKLAELPPWNPIPNNVTEELQQEENNAGTRDMVIAAENSES SGYNFQLQDTENHHNHSAFIPSNIDTDAIAFFPTSSAASSINFQSYPSDIISRTNNSIQD LGLSLHSFQDHGMMHGQSQEGGNHQTGSNDHHQTLFTDSNPVGFEANYHRIVNWNNDDHR TGFMVNSAPFLSQGSAFSHRGTLQSSFSQSLRPWNDIPMETSSVNHNQKSQQIQQASIFG SRFLSDALPGFCIPARIQGEDESHVVVSDRPSSSSNSHH* >CaTCP03 MELSDLQNNKQKRGTHQQQQQESSSSPSNSHSHSHSHSHQQHLVVPFDGRPPPFIMGSIS IQQPSSNSSSSSSSNSSSSAIDASLAIATRSEPPLLNNTNNQLSSSSPAVTTVANPPKRS TKDRHTKVDGRGRRIRMPATCAARVFQLTRELGHKSDGETIEWLLQQAEPAIIAATGTGT IPANFSTLNVSLRSSGSTLSAPPSKSAPHSFHGALALTRHPYEEAAYQHAPLLGFHQHHQ QLMSADQIGEALNPNENYMRKRYREDLFKDDNNNNESGGDGSSSSPKAFKTNHQQQDTMG EGEGGGGGGGGGGSGASSSSSSNSSISGLLRPSNMIPATAMWATAGSGNTIWMVPVSGGG GGAATSNSETQTQNMWPFGPSPSQNSQTQGNNAGTPSPLHFMPRFNLPVSGGGGGGGVEF QGGRGGLQLGSVYQPSQHIGLAVSDSNLGMLAALNAYTRAAAGATDFNVNVNSDNHHHHH HQHQHQHHSISLDSQSHSHSHSHSHPQPTESEEDAPDTSQ* >CaTCP04 MSGEIVEVDGGHIIRSRGRKDRHSKVCTAKGPRDRRVRLSAHTAIQFYDVQDRLGYDRPS KALDWLINKAKPAIDQLAQLPPWKPTLLTKQQQNDDVPQNQNEHEFHFLQNFNSNNNNFL PFDTETTSSSPIHFQSYSTHDDLRLSLQQHQNQHVHPHVLFAGNLDGMVAWNSGATIDTG SNNNNNNHHHDDVNSGGGFVFHAATPSPAVFPAVMYGQNQYLSQRGPLQSSNTSSVRAWI DAPTFVAADHRRGHAVAMSGTGFVSDSFSGFRVPARIGGDEEEEHDGLSDRPSSASSDSR R* >CaTCP05 MINIQKEEDFPLKAKASTISSSSSSSQWLQLKDHPRIVRVSRAFGGKDRHSKVCTIKGLR DRRVRLSIQTAIHLYDLQDRLGLNQPSKVVDWLLNVAKDEIDELPPLQIPQENFTLGYPS FNDDNTSIWKSNPKQVLGEMVNHKENWINKSEEDKVENNDFHDAQHVLTNQPSFLGLLNN SMPLAYHQLQPSIRDIHYGINVVPFPSSLSLSTQQYFPSHVVGAMDMDPRQINYNYDQMM SSSSQNKFSQSNSTIHHEGGS* >CaTCP06 MELEADHHQNNGIRSRPNFPLQLLEKKDIIDDISEQPCSTTANDGCSAIVTSNNTADQQK KPPPKRASTKDRHTKVDGRGRRIRMPAACAARVFQLTRELGHKSDGETIEWLLQQAEPAV IAATGTGTIPANFTSLNISLRSSGSTMSAPSHYFRGNYFNPSSFSSAAAAAAQVRSRAEW ERTININNNINMNVVAEDSRRTNSTNMLENHNPSSLSAILNFNNVQNAILQAKQELREEN 140 AAAGGGGGFESVASDSDGNIGRKRRSEQEFLSQNMGSYLLQSNVGSLPSSHASNTAAFWM VTGHGNQGVNVGGGGNGNEPIWAIPSVGNSGNLYRGAMSSPNGIHFMNFGSPMSLMPGSQ LGSGIVGVGGGNGGFLAESNLNMLSAMNAYRQINSTNCVSDSSGQHHGGGGSGRDDGHDS TSQHS* >CaTCP07 MEGNHTHDDDFSEENETQSTSETTEPQTLNTPEPTSVMKEELTETERHGIDESLSVGVLP MPMPLTVSAAKKPSKDRHTKVEGRGRRIRMPATCAARIFQLTRELGHKSDGETIRWLLEQ AEPSIIEATGTGTVPAIAVSVGGTLKIPTSSPARPDGEEAPKKRRRRASNSEFIDVNEQV SMCSGLAPIAQTAYGGAAGGSGGGIVPLWPVVSSGGSNATGPFFMFPNSTTNQPQFWAIP AAPFFNVQNRPISDFVSAMHMQQPLNVASAFGNTNCSSTLAPSLSSANTTTTTCSSAATS SSSSQMLRDFSLEIYDKKELQFMDSSASNLEPPPTSN* >CaTCP08 MTSELVIHTFSQPQTNKPSTATASDPNSQISTVKRTSTTTASRSTKDRHTKVNGRGRRVR MPPLCAARIFQLTRELGHRSDGETIEWLLRHAEPSIIAATGTGTVPAAPVSTVSPVSSSS VPSVSCHVQPVSSVGPGGQGMFAAAMSQPQPSCRLDLCQPPGMEYAAANGYRHMPFTALL LQPTTAEENQQEEALSEQ* >CaTCP09 MSNCKETTNAVTVTNGTTTDSQKTIAVKKPPSKDRHSKVDGRGRRIRMPIICAARVFQLT RELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSSLSEHKPFHPPSPFILGKRLRSE QDDAVSVAAPTAPPALWALPSRPDFAQLWSFAAAAAQPPSEVLSISPQQQQHNSLFHHQQ QQQQQHQASMGEASAARLGNYLPGHLNLLASLSGNGNSGRGDDEHH* >CaTCP10 MSNCKETTNAVTITNGATTTTTEPETTQRNTIAVKKPPSKDRHSKVDGRGRRIRMPIICA ARVFQLTRELGHKSDGQTIEWLLRQAEPSIIAATGTGTTPASFSSLSEHKPFLPPSPFIL GKRLRSEQDDAVAVAAHPAPPALWRPDFAQLWSFAAAAQPPPEVLSISPQQQQHNSLFHH QQQQQQQQHQASMGEASAARLGNYLPGHLNLLASLSGGNGNSGRDDDEHR* >CaTCP11 MFSSTYHDSNPLPNFPSSSSSSYPTLSFLIDPQNDFPNNTLLDDPLSLPFLPSTTTTHDV LPIFPNETTINANNLATSLFEQDISTSTNVNNCNSNYGVSVVPNFVTQKLPSSTIGKKDR HSKIHTSQGLRDRRVRLSSEIARKFFDLQDMLEFDKPSNTLEWLFTKSESAIKELARTKN IGCCNNNNPFEYDTNNNNNHKNSSFSGVGDNKQGRNKLKWTQQKEEAFVVQTKKESRERA RARARERTCYKMCSTSTNGSSTNHHHHNHNQMLQQLMMSSSSPIDHQSSTENDEAYARWR QHLVQLQTYHPSTYSHHNLLDESEIPRDHYGFNVIEESIMIKRNMMSSTSSHHHHQQNLA TIPKEPTFNNNNIIDYPNLFPYSTTNWETNGGCSNFYGIATMNLSTCFMNQ* >CaTCP12 MIKNSKEALKQEGHSNSNNDEKEKASSSNSLASQWSRLKDPRIVRVSRAFGGKDRHSKVC TIRGLRDRRVRLSVPTAIQLYDLQDRLGLNQPSKVVDWLLNVAKHEIDELPPLPNFPSHG NFNFGFPSINIEANTNTSQLNQQLLNINRNIPWEGSTSSQNSMWKLKPKEVTQEMVIDNK ANYWMNRIEEDDDNNNKHGSNEGNNSSDHVLSSNNNNLLLQRTNHQASFLGLLNTMPSMG NYQWGGNSNVDVNSQLGNHIGFTNQTNVNVVTSLPPMLSLSTGNSQMMPYFPSHHHVNAM EMDNHHHQRQINHHNYQMLSSSSQNPLNNSLNNPSFNLAMMIPKENPSNKDQDFTPK* >CaTCP13 MEEDEIQVQSQNQSCKFPRVGNNRNDQEEDLEDGSMRRVGGCDGNTNRLNNWHHSSRIIR VSRASGGKDRHSKVMTSKGLRDRRVRLSVTTAIQFYDLQDRLGYDQPSKAVEWLIKSASD AISELPSLNNTFPNDDDTNQLLQPSSEQQQGLDSADAEMIENGENNNYHQNQNNQNNLCL SKSGCSSTSETSKGSGLSLSRCDIRVSRVKARARARERTAKEKEKENNECSHHHQQQQQH QISQTASFTELLTGGINNAVANSPRGSSIDEADLFNKARQQQWNSAAPMDHYFNSVLLGP SSSSSSRTHHQSLSQIQLGHSLHEQHHQSMSFSNNSGENQNHQMQQQQQQHQFSFIPDNN 141 DYNLNFKISSSLGLAGYNRGTLQSNSLSHLQRFSVSPIDHGSNVVVPFFMGGGGGGGASS ATTASPSPSDATPTMENNHQIHHHHHQFSSVFDGSRLQLYSDHKGKPKN* >CaTCP14 MRRSSRGGEIVEVEGGHIVRSTGKKDRHSKVYTSKGPRDRRVRLSAHTAIEFYDVQDRLG YDRPSKAVDWLIKKAKHSIDKLADLPPWHPSSDNQTQQQQNVAAGSTSLVIGEQQSQSNS GYNFHLLRHFTDNQCDNNNSSFISQHAVETDPIPFFPTTSSTSQPINFQTYPHHHGIIST NNIHSTQDLGLSLHSFQDHNGLINPWQSQQQDCQNHATSNQNQTLFAGFENQYQRIVNWN SETTTDYVKKVGFMVNSQQFLPHGSTSASEYSPNETLQSNFSPSVRSWNDIPMASSEHHR TEQVHQASIFGSRFVCDGLAGFCVPDTVQGDEEGHGVSSNRPSSASPTSHHH* >CaTCP15 MYSSNSSLNGNNNNLTGTSFCSSTPFTFESNSSSSTSKHHQNTCFPPSPFSFIQFPYDTF EDNNQIFLLQEQDDHIQMNVKNKEEEEDKGKTVLVVVDEHKKNVQRKRSSKRDRHSKIKT AKGLRDRRMRLSLDVAKKFFGLQDMLGFEKASKTVEWLLNKSKLEIKQLAREKNLQNDSS STTSECEEGVSSLDNNDHQQQQKIMLVKRSSSTNKVSRKSAFNCIGREKARERARERTRQ KLNARIRDVNIVDQSNSKQCVNNNNNNNNWNPFEECAVNNSSFDVKMINHEAKEHSELED EENSLFINMTKWSPTFMFNNSSILQQHHQYAQFQCLEKPWEDYNNTI* >CaTCP16 MDGGDDLHHHHQHQHHHHHHHQQQRQNFPFQLLEKNEDQEAASCSTSSPYPSLAISPTEP TTSNSNRSNQLVSAPTTSSEPGNKKPPPKRTSTKDRHTKVEGRGRRIRMPALCAARVFQL TRELGHKSDGETIEWLLQQAEPAVIAATGTGTIPANFTSLNISLRSSGSSMSVPSQLRSS YFNPNFSLQQRRSLFPGIGLSSSDNNSNNNTSTLLNFQTNNLTTNMLQAKQELRDGGGGG GGGAPSSSTTLDLSETSGEENMGRKRRPSEQDLTSSTTSQQQMGSYLLQSSAGSIPASHT QIPANIWMVANSNNHQVMSGDPIWTFPSVNNSAAAAAALYRGAVSTSGLHFMNFPPPMAL LPGQQLSNSSLGGNSSGGGGGGNINMNMSMNMNMNEGHLSMLAGLSPYRPVSHHESHQQG SGSQSQRGGSHDRHDNTSHHS* >CaTCP17 MGETTQDNNNTGGAGTATTTSKRIGGGEIVEVQGGHIVRSTGRKDRHSKVCTAKGPRDRR VRLSAHTAIQFYDVQDRLGYDRPSKAVDWLIKKAKASIDELAQLPAWNPTATTPPSSTPQ QHHQHTDEMFHRQSPVHDDPVTGSTSRRAAALERRIPQQFSSQQLELEDNNNNNNNNSGK HKYSNASTSGFLQGSLDTDIADTIKSFFPVDTATTSFHSYPPPPSDLLSRTPTTQQDLRL SLQSFQDPIMLNHQAQNHHVLFAGASSLGFDGGGWPEQQHHEEHDHGRLHRMMAWNANAV DGGNSGHGGGFVFTAPTPAFGGHAQFFTQRGPLQSSNTPSFRAWIDPSMATATIADHHNH HYLSPMIHQASVSGGGFSIPARIQGEEEHDGVSEKPSSASSDSRH* >CaTCP18 MSQEGSRNIDLTLGEPKEEPETEDGSAINVMPMAMHVPTGISMSMQVNKALIQAQAQAQT QVIQHQQPLQKRASTKDRHTKVEGRGRRIRMPATCAARIFQLTRELGHKSDGETIRWLLE HAEPAIIAATGTGTVPAIAMSVNGTLKIPTTSPNKSSDPEQNQQQLEDSPDKKKRKRPVN SAYVDINDSTVSVSAGLITTSISNANINTNLTTTAIIPQQTAIPLPQNMVPVWAIPSNAV VPAGSAFFVVPTNQPQFFTLARPISAFVSSMVTPVQIQQTSTSSNTCSISPPSKSPARAS VMAPTAAATTTTQMLRDFSLEIYDRQELQLLSRSSSKH* >CaTCP19 MMIENSSESYEAKQEGGDGIMKSEKLAGAGSTSRQWTSLRNPRIVRVSRSLGGKDRHSKV CTIRGLRDRRIRLSVPTAIQLYDLQDRLGLSQPSKVVDWLIEATQSDIDKLPPLDIPQCF TSHFHHHQLPPPGTSFSLGGFYDQNNGMAKTRYNNWDIDSSVGRLKGKEAENVCMDSEKS KLWMKSTNEQFGSNYFSNSSPSVHGLSSYHNLDPSSTLSLSQFGNSGNNIFPYYHQQVES HHNSGTVMQFSSSSFTNGNSQLLFCPSSVSLFSNNNNSLETTDNHAARQHLHHQILNSSS SSLSSSSSSSHALMMPLIPTLHLPINSPFRRLPLPFSSKLLDSDHDNNNTRNG* >CaTCP20 142 MFPFNSNPYPSFVPNSSASSSSLFSFPFLNPENASSSNNSNNNTLFLDPFSIPYIPTHHH HHPHHHNITNIQETLTNLAENNNNSNNNSNSNNNNNNNNIAIPMAKQDPSTIGGGSHYGI SCFLAKKPPKKDRHSKIYTSQGLRDRRVRLSIEIARKFFDLQDMLGFDKASNTLDWLFTK SKKAIKDLTKSKQKNNNEDGDDDDEDAKSFTSSSDCEVVSDIKQHSSNLKQNQDGDGDFS KEKKLKRAQIKESSCIRANKMKESREKARARARERTSNKISNNNNTLKIHQLELKKKYPT TNENFQAFHQSRSSLQDHQLVSSSETPRDDFNVIEESIVIKRKLKPSLMVSHHHHHQHQN HVIPKEATNFNNSEYHSYPNSSPNWDASNNSASSSNRSTFCAIASMNLSTGLQIFGKSWE ECTNPRLH* >CaTCP21 MDPKSSKQQSQEPNNNMGENKPSDSKDFQIVLSETTTKDETTKKQQQLAPKRTSNKDRHT KVEGRGRRIRMPALCAARIFQLTRELGHKSDGETIQWLLQQSEPSIIAATGTGTIPASAL SSSSVTTQQQGSSSCSLSSGLQLHQKIDELVGNRITSSNSWGTMVGGNLGRTNMWTPHHH VSGFGFQSSSAHSTTTNDHGSNYLSKIGFSGFDHQNINSNNNNNNGNMVGGMSFTSILGG NHQQIPGLELGLSQDGQHIGVLNQQALSQIYQQIGQTRVHHQQQQNQNQNSSKDDSQGSG Q* >CaTCP22 MASSREGYEAKQEGGENSNNNSIEKISKAASTSRQWSSTGLRNPRIVRVSRTFGGKDRHS KVCTIRGLRDRRIRLSVPTAIQLYDLQDKLGLGQPSKVIDWLLEATKFDIDKLPPLQFPQ GFSQFHHPQTLLPFHESITSASHELSLGPLYDPSSSFVGIQNLMARSKFWEMDSRVNNKG KEVEREYFVEKGKWIKTNEEENHEASYNNFQQTSTQKLFPMETHSTLPSFLNNNAMSYNN YHLEPSNLSLSQFGGSHGLFQSQQVDPNQSSGNGLQFPFSFPLQQSTSQLLFGPSSSSTS PSSSSLLSNPFMTTTNSVENDPRVQQFNHFQFINSSSSSSQAMTHPLLPSFHSFNSPAVR PFPMPFSSKLLDLDNNHNGNQHDNKGCRGDRS* >CaTCP23 MFPSTYCSLGPYPCYTSSSSSHSYPPFTFLTPENASSNTNNNNNNNMNTFLHDPVITIPY TQTTHHHVPIPESLTNWAVSDYATMLKQDLSHHHGSSYGFTNLLTKKQVKKPAKKDRHSK IHTSQGLRDRRVRLSIEIARKFFDLQDMLGFDKASNTLEWLFNKSKEAIEELTISKNPSA NVDDYDANDDVNNDDDSFSSSSCEDMIDNKERKMKRVVKETSKMKDLRGKARARAREXXX XRRCEDLKEKYVESENPQQILHQLMPNDDENNISKSQQPRLVHHLGVGGGEFQRDDIFNF IEESIVIKRKLKKSSVSSSSNSHHQQNNLISKEPLGFDNHNNDCHESTMSPNWDANNAGR SNFSSISRMNLSSGLQIFGKSWEECNSSPNNRY* 143 Phụ lục Kết giải trình tự gen CaTCP07 sau chuyển plasmid pENTR/D-TOPO TCP7-11_M13 Sequence : CaTCP07.prj Size : 1014 Matching Position : - 1014 Sequence : pENTR TCP 7-11_M13 F.seq Size : 1107 Matching Position : - 1107 : GGACAAATAA TGAATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA : -A TGGAAGGGAA TCATACCCAC GACGATGATT TCAGCGAAGA AAACGAAACA CAAAGTACAT CGGAGACAAC * ********** ********** ********** ********** ********** ********** ********** GGCTCCGCGG CCGCCCCTTG GATCCCACCA TGGAAGGGAA TCATACCCAC GACGATGATT TCAGCGAAGA AAACGAAACA CAAAGTACAT CGGAGACAAC Primer F 101 : 72 : 201 : 172 : 301 : 272 : 401 : 372 : 501 : 472 : 601 : 572 : 701 : 671 : 801 : 771 : 900 : 871 : 992 : 971 : 1075 : AGAACCGCAA ACGTTGAATA CGCCGGAACC AACTTCGGTA ATGAAAGAAG AGCTAACGGA AACGGAACGT CATGGCATCG ACGAGTCACT TTCTGTTGGT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** AGAACCGCAA ACGTTGAATA CGCCGGAACC AACTTCGGTA ATGAAAGAAG AGCTAACGGA AACGGAACGT CATGGCATCG ACGAGTCACT TTCTGTTGGT GTTCTACCTA TGCCGATGCC ATTAACAGTA TCGGCGGCGA AGAAACCGTC GAAAGACCGT CATACGAAGG TGGAAGGTCG TGGCCGGAGA ATCCGAATGC ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** GTTCTACCTA TGCCGATGCC ATTAACAGTA TCGGCGGCGA AGAAACCGTC GAAAGACCGT CATACGAAGG TGGAAGGTCG TGGCCGGAGA ATCCGAATGC CGGCAACATG TGCCGCCAGA ATATTCCAAC TAACACGGGA GCTTGGTCAC AAATCCGACG GAGAAACAAT ACGGTGGTTG CTAGAACAAG CTGAACCTTC ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** CGGCAACATG TGCCGCCAGA ATATTCCAAC TAACACGGGA GCTTGGTCAC AAATCCGACG GAGAAACAAT ACGGTGGTTG CTAGAACAAG CTGAACCTTC CATCATAGAA GCAACTGGCA CCGGAACAGT TCCTGCCATC GCCGTTTCAG TCGGCGGCAC GTTAAAGATT CCAACTTCAT CACCGGCGAG ACCAGACGGT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** CATCATAGAA GCAACTGGCA CCGGAACAGT TCCTGCCATC GCCGTTTCAG TCGGCGGCAC GTTAAAGATT CCAACTTCAT CACCGGCGAG ACCAGACGGT GAAGAAGCTC CAAAGAAACG AAGGAGAAGA GCATCAAATA GTGAATTTAT TGATGTGAAT GAACAAGTTT CAATGTGTTC AGGGTTAGCA CCAATTGCAC ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** GAAGAAGCTC CAAAGAAACG AAGGAGAAGA GCATCAAATA GTGAATTTAT TGATGTGAAT GAACAAGTTT CAATGTGTTC AGGGTTAGCA CCAATTGCAC AAACTGCATA CGGCGGTGCT GCCGGTGGAA GTGGTGGTGG AATAGTCCCT TTATGGCCTG TGGTAAGTAG CGGTGGTTCA AATGCAACAG GACCA-TTTT ********** ********** ********** ********** ********** ********** ********** ********** ********** ***** **** AAACTGCATA CGGCGGTGCT GCCGGTGGAA GTGGTGGTGG AATAGTCCCT TTATGGCCTG TGGTAAGTAG CGGTGGTTCA AATGCAACAG GACCATTTTT TTATGTTCCC TAATTCAACT ACGAATCAGC CACAATTTTG GGCTATTCCT GCGGCACCGT TTTTTAATGT TCAAAATAGA CCCATTTCAG ATTTTGTTTC ********** ********** ********** ********** ********** ********** ********** ********** ********** * ******** TTATGTTCCC TAATTCAACT ACGAATCAGC CACAATTTTG GGCTATTCCT GCGGCACCGT TTTTTAATGT TCAAAATAGA CCCATTTCAG A-TTTGTTTC TGCTATGCAT ATGCAACAAC CTTTGAATGT TGCTTCGGCT TTTGGAAATA CTAATTGTTC GTCTACTTTG GCTCCTAGTT TGAGCTCAGC AAATACTACT ********** **** ***** ********** ********** *** ****** **** ***** ******** *** **** * ********** ********* TGCTATGCAT ATGC-ACAAC CTTTGAATGT TGCTTCGGCT TTT-GAAATA CTAA-TGTTC GTCTACTT GCT-CTAG-T TGAGCTCAGC -AATACTACT ACAACTACTT GTTCTTCTGC TGCTACTTCT TCATCTTCAT CTCAGATGCT AAGGGATTTC TCTTTGGAAA TTTATGATAA GAAAGAGCTT CAATTTATGG ** ****** ** ***** ********* ***** **** *** ****** * ****** **** * ** *** *** * **** *** *** **** AC-ACTACTG GT CTCTGC TGCTACTTC- TCATC-TCAT CTC-GATGCT A -GATTTC TCTTAG AA GTTA-GAT GGAAGA-CTT CAA-GTATG- ATTCTTCTGC TTCAAATTTA GAACCACCCC CTACTTCAAA TTAA *** ** * **** * * * ** * * * ** * -ATCT CGC T CAATTGA CATCTACTC- -ATAGA TTCA 144 1st Nucleotide Sequence File Name : CaTCP07.prj Sequence Size : 1014 2nd Nucleotide Sequence File Name : pENTR TCP 7-11_M13 R.seq (Complementary) Sequence Size : 1165 1' ATGGAAGGGA ATCATACCCA C-GACG-ATG A-TTTCAGCG AAGAAAACGA AACACAAAGT ACATCGGAGA CAACAGAACC GCAAACGTTG AATACGCCGG **** * * ** *** * **** ** *** * ** * *** **** * *** *** * **** ** *** ** ** ****** ** 1" TCCCCCA CGGCCGAATG ATTTTCTACG GAGATATCG- ACCAC-AAGT A-ATC-GAGT C-ACAG-ACG GCATACCGTG -ATACGCGGG 98' AACCAAC - TTCG-GTAAT GAAAG-AAGA GCT-AACGG- -AAACGGAA- CGT-CATGGC A-TCGAC-GA GTCA-CTTTC TGTTG GTG TTCTACC-TA **** ** * ** ***** * *** **** *** * *** ** ***** *** ****** * ***** ** **** ***** ** ** ** ******* ** 81" AACCCACTCT TCCGCGTAAT GCAAGTAAGA GCTCAGCGGC AAACCGGAAC CGTCCATGGC AGTCGACTGA GTCACCTTTC TGGTGTGTTG TTCTACCGTA 182' TGCCGATG-C CATTAACAGT ATCGG-CGGC GAAGAAACC- GTCGAAAGAC CGTCATACGA AGGTGGAA-G GTCGTGGCCG GAGAATCCGA ATG-CCGGCA ******** * ********** ***** ** * ** *** ** ********** ********** ******** * ********** ********** *** ****** 181" TGCCGATGCC CATTAACAGT ATCGGCCGAC GATGAATCCG GTCGAAAGAC CGTCATACGA AGGTGGAAGG GTCGTGGCCG GAGAATCCGA ATGCCCGGCA 277' ACATGTGCCG CCAGAATATT CCAACTAACA CGGGAGCTTG GTCACAAATC CGACGGAGAA ACAATACGGT GGTTGCTAGA ACAAGCTGAA CCTTCCATCA ********* ********** **** *** ********** ********** ********** ********** ***** **** ********** ********** 281" GCATGTGCCG CCAGAATATT CCAAACTACA CGGGAGCTTG GTCACAAATC CGACGGAGAA ACAATACGGT GGTTGATAGA ACAAGCTGAA CCTTCCATCA 377' TAGAAGCAAC TGGCACCGGA ACAGTTCCTG CCATCGCCGT TTCAGTCGGC GGCACGTTAA AGATTCCAAC TTCATCACCG GCGAGACCAG ACGGTGAAGA ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** 381" TAGAAGCAAC TGGCACCGGA ACAGTTCCTG CCATCGCCGT TTCAGTCGGC GGCACGTTAA AGATTCCAAC TTCATCACCG GCGAGACCAG ACGGTGAAGA 477' AGCTCCAAAG AAACGAAGGA GAAGAGCATC AAATAGTGAA TTTATTGATG TGAATGAACA AGTTTCAATG TGTTCAGGGT TAGCACCAAT TGCACAAACT ********** ********** ********** ********** ********** ********** **** ***** ********** ********** ********** 481" AGCTCCAAAG AAACGAAGGA GAAGAGCATC AAATAGTGAA TTTATTGATG TGAATGAACA AGTTACAATG TGTTCAGGGT TAGCACCAAT TGCACAAACT 577' GCATACGGCG GTGCTGCCGG TGGAAGTGGT GGTGGAATAG TCCCTTTATG GCCTGTGGTA AGTAGCGGTG GTTCAAATGC AACAGGACCA TTTTTTATGT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** 581" GCATACGGCG GTGCTGCCGG TGGAAGTGGT GGTGGAATAG TCCCTTTATG GCCTGTGGTA AGTAGCGGTG GTTCAAATGC AACAGGACCA TTTTTTATGT 677' TCCCTAATTC AACTACGAAT CAGCCACAAT TTTGGGCTAT TCCTGCGGCA CCGTTTTTTA ATGTTCAAAA TAGACCCATT TCAGATTTTG TTTCTGCTAT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** 681" TCCCTAATTC AACTACGAAT CAGCCACAAT TTTGGGCTAT TCCTGCGGCA CCGTTTTTTA ATGTTCAAAA TAGACCCATT TCAGATTTTG TTTCTGCTAT 777' GCATATGCAA CAACCTTTGA ATGTTGCTTC GGCTTTTGGA AATACTAATT GTTCGTCTAC TTTGGCTCCT AGTTTGAGCT CAGCAAATAC TACTACAACT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** 781" GCATATGCAA CAACCTTTGA ATGTTGCTTC GGCTTTTGGA AATACTAATT GTTCGTCTAC TTTGGCTCCT AGTTTGAGCT CAGCAAATAC TACTACAACT 877' ACTTGTTCTT CTGCTGCTAC TTCTTCATCT TCATCTCAGA TGCTAAGGGA TTTCTCTTTG GAAATTTATG ATAAGAAAGA GCTTCAATTT ATGGATTCTT ********** ********** ********** ********** ********** ********** ********** ********** ********** ********** 881" ACTTGTTCTT CTGCTGCTAC TTCTTCATCT TCATCTCAGA TGCTAAGGGA TTTCTCTTTG GAAATTTATG ATAAGAAAGA GCTTCAATTT ATGGATTCTT 977' CTGCTTCAAA TTTAGAACCA CCCCCTACTT CAAATTAA ********** ********** ********** ******** 981" CTGCTTCAAA TTTAGAACCA CCCCCTACTT CAAATTAAGA TATCAAGGGT GGGCGCGCCG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA Primer R (complementtary)