Development of a quechers based methodcombined with gas chromatographymass spectrometry for the analysis ofalkanes in sediments

SAMPLE PREPARATION TECHNIQUES IN ANALYTICAL CHEMISTRY GROUP Development of a QuEChERS-based method combined with gas chromatography mass spectrometry for the analysis of alkanes in sediments Lecturer: PhD Tran Thi Thanh Thuy MEMBER ASSIGNMENTS MEMBERS ID TASKS Pham Thi Thu Thao 20002021 Prepare Introduction, edit ppt Le Tan Bao 20001931 Prepare Scope of application Ho Ngoc Kieu 20005051 Prepare Meaning of method, present Tran Thi Hong Phuc 20005541 Prepare Principle of method Ho Hong Phuc 20001841 Prepare QuEChERS Extraction, present Tran Anh Tu 20035401 Summarize contents, edit ppt CONTENTS INTRODUCTION MEANING OF METHOD QuEChERS EXTRACTION APPLICATION PRINCIPLE OF METHOD INTRODUCTION • Was introduced in 2002 QuEChERS Quick ✓Simple Easy Cheap ✓Effective Effective Rugged High quality ✓ Safe results •  A type of dispersive solid phase extraction (dSPE) used for sample preparation • Determine 29n – alkanes (C12 – C40) and two isoprenoid acyclic hydrocarbons (pristane and phytane) in sediments 2 APPLICATION Extract alkanes from C22  to C34  with an odd to even predominance, highest in C31 This is consistent with a vascular land plant input These results can be used for further paleoclimate investigations Lady-fern from Vascular plants MEANING OF METHOD Develop a method: ✓ ⏳ Low cost ✓ ⏳ ✓ Reduction of solvent consumption and possible associated contamination Short analytical time ✓ Avoid the need for sophisticated devices ✓ Diminish possible auto-oxidation reactions Alkane extraction by QuEChERS method would be more in line with the principles of Green Analytical Chemistry 4 PRINCIPLE OF METHOD Partitioning dSPE clean up ACN LC-MS • The analyte in the sample is extracted in a suitable solvent • The analyte in the extract is cleaned with an impurity adsorption 5 QuEChERS EXTRACTION SAMPLE SELECTION  Samples were collected from archaeological sites in  Austria and Spain  Samples is described as homogeneous beige silt, approximately 1.30 m below surface  Collected by metal tools   rinsed with MeOH and DCM   ⇒ wrapped in aluminum foil   ⇒ stored at -20°C Sample extraction Clean up Extraction REFERENCE  Antonio V Herrera-Herreraa, Naima MohamedRodrgueza, Brbara Socas-Rodrguezb, Carolina Mallol Development of a QuEChERS-based method combined with gas chromatography-mass spectrometry for the analysis of alkanes in sediments