(Luận Văn) Determination Of Ammonia In Water Samples Using Nda Fluorescence Derivatization With Different Nucleophiles.pdf

THAI NGUYEN UNIVERSITY UNIVERSITY OF AGRICULTURE AND FORESTRY an lu n va to p ie gh tn PHẠM THỊ HẢI VÂN w TOPIC TITLE: DETERMINATION OF AMMONIA IN WATER SAMPLES d oa nl USING NDA FLUORESCENCE DERIVATIZATION WITH DIFFERENT NUCLEOPHILES v an lu m ll fu an BACHELOR THESIS : Full-time Major : Environmental Science and Management Faculty : International Training and Development Center Batch : 2011-2015 oi Study Mode nh at z z @ om l.c gm an Lu Thai Nguyen, 9/2015 n va a th i c si Thai Nguyen University of Agriculture and Forestry Degree Program : Bachelor of Environmental Science and Management Student name : Phạm Thị Hải Vân Student ID : DTN 1153070072 Determination of Ammonia in Water Samples Using NDA Thesis Title : Fluorescence Derivatization with Different Nucleophiles - Assoc.Prof WU ,Chien-Hou Supervisor (s): an lu - Assoc.Prof Dr Do Thi Lan n va Abstract: Fluorescence has been in use by physical chemists for many years gh tn to Based on emission capacity of substance that has absorbed light or other electromagnetic radiation, fluorescence seems as one method with highest p ie applicability in chemical analysis An attraction of fluorescence is its sensitivity, helps scientists save w which time in research Based on that d oa nl advantage, fluorescence has widely application for determining ammonia and other possible nitrogen-containing photoproduct compounds (amine, amino acid ) v an lu with derivatizing reagent, OPA (o-phthaldialdehyde) On the other hand, fu an naphthalene-2,3-Dicarboxaldehyde (NDA) has been estimated as another popular oi OPA derivatives(Carlson, 1986) m ll derivatizing reagent, that can form much more stable derivatives with amines than nh This study was to optimize the derivatization conditions including concentration at z of NDA, maximum excitation and emission wavelengths, reaction pH, reaction z @ temperature and time, and choice of nucleophiles for analysis of ammonia in aqueous gm Fluorescence, Naphthalene-2,3-dicarboxaldehyde (NDA), Ammonia, Cyanide, Sulfite 53 n a th ii va September, 30, 2015 an Date of Submission: Lu Number of Pages: om l.c solution Keywords: c si ACKNOWLEDGEMENT To have completed this thesis, in addition to the ongoing efforts of myself, I would like to thank for teachers in faculty of International Training and Development as well as teachers in Thai Nguyen University of Agriculture and Forestry, who have dedicated teaching to me the valuable knowledge during study time in university and gave me a chance to my thesis oversea It is with immense gratitude that I acknowledge the support and help of Biomedical Engineering & Environmental Science Department, an lu National Tsing Hua University for accepting me to working in this wonderful place va n Furthermore, express my sincere deepest gratitude to Assoc Prof Wu Chien Hou, to gh tn from Biomedical Engineering & Environmental Science Department, National Tsing Hua p ie University and Assoc.Prof Dr Do Thi Lan from Thai Nguyen University of Agriculture d oa nl w and Forestry, who directly guided and created favorable conditions for me during the implementation of this thesis v an lu Next, i would like spend special thanks to Mr Bill (Wang Chin Yi) PhD student and my friends in the laboratory facilitated and provided the information and data fu an necessary for my implementation process and helped me finish this thesis m ll Finally, I would like to sincerely thank my family, all of my friends who always oi nh beside me all the time, giving spiritual help for me complete the tasks assigned during at z learning and research doing this thesis experiment z @ In the process of implementing the project, my thesis might have inevitable gm l.c shortcomings Therefore, would like to receive the attention and feedback from teachers om and friends to this thesis is more complete an Lu Sincerely, a th iii n va Pham Thi Hai Van c si TABLE OF CONTENT LIST OF FIGURES LIST OF TABLE PART I INTRODUCTION 1.1.Research rationale 1.2.Objectives of the research 1.3.Research questions and hypothesis lu an 1.4.Limitations of research n va PART II LITERATURE REVIEW gh tn to 2.1 Ammonia p ie 2.1.1 The properties of ammonia w 2.1.2 Origins of ammonia pollution in water d oa nl 2.2.Methods for determinate ammonia v an lu 2.2.1.Ion Chromatography m ll fu an 2.2.2.Voltammetry 10 2.2.3Fluorescence 11 oi nh 2.3.Overview of research and application of NDA in fluorescence 12 at z 2.3.1.o-phthaldialdehyde (OPA) 12 z @ 2.3.2.Naphthalene-2,3-dicarboxaldehyde (NDA) 14 gm om l.c PART III METHODS 16 3.1 Material 16 Lu an 3.1.1 Chemicals 16 n va a th iv c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles 3.1.2 Equipment 16 3.2 Methods 18 3.2.1 Determine the optimum concentration 18 3.2.2 Fluorescence Spectrophotometer 21 3.3 HPLC system 24 PART IV RESULTS 27 4.1 NDA-ammonia-sulfite reaction 27 an lu 4.1.1 Optimization Excitation and Emission wavelength 27 n va -sulfite product 28 gh tn to 4.1.2 Effect of pH value on the fluorescence of NDA4.1.3 Effect of reaction time on the fluorescence of NDA- -sulfite product 29 p ie 4.1.4 Effect of reaction temperature on the fluorescence of NDA- -sulfite product 30 w -sulfite d oa nl 4.1.5 Effect of ratio between NDA and sulfite on the fluorescence of NDA- product 31 v an lu 4.1.6 Apply Pertinent Parameters on determine ammonia 33 fu an 4.2 NDA-ammonia-cyanide reaction 34 m ll oi 4.2.1 Optimization Excitation and Emission wavelength 34 nh -Cyanide product 35 4.2.2 Effect of reaction time on the fluorescence of NDA- at z -Cyanide product36 z 4.2.3 Effect of reaction temperature on the fluorescence of NDA- @ -sulfite gm 4.2.4 Effect of ratio between NDA and cyanide on the fluorescence of NDA- om l.c product 37 an Lu 4.2.5 Apply Pertinent Parameters on determine ammonia 38 4.3 Determine ammonia using HPLC-fluorescence system 40 n va a th v c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles 4.3.1 NDA-ammonia-sulfite reaction 40 4.3.2 NDA-ammonia-cyanide reaction 42 PART V DISCUSSION AND CONCLUSION 45 5.1 Discussion 45 5.2 Conclusion 46 REFERENCES 47 an lu n va p ie gh tn to d oa nl w oi m ll fu an v an lu nh at z z @ om l.c gm an Lu n va a th vi c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles LIST OF FIGURES Figures Page an lu Figure 2.1 Synthesis NDA 15 Figure3.1 Fluorescence Spectrophotometer System 17 Figure 3.2 HPLC Fluorescence System Diagram 18 n va Figure 3.3 Warm Up Sample Solution gh tn to 20 Figure4.1 Excitation And Emission Of NDA-Ammonia-Sulfite Reaction p ie 27 w Figure Effect Of Reaction Ph On The Fluorescence Of NDA-NH 28 d oa nl Sulfite Product v an lu Figure Effect Of Reaction Time On The Fluorescence Of NDA-NH 29 m ll fu an Sulfite Product Figure 4 Effect Of Reaction Temperature On The Fluorescence Of NDA30 oi nh NH -Sulfite Product at z Figure Effect Of Ratio Between NDA And Sulfite At NDA=0.5mm z 31 @ gm Figure Effect Of Ratio Between NDA And Sulfite At NDA=1mm om l.c 32 Figure Effect Of Ratio Between NDA And Sulfite At NDA=2mm an Lu 32 n va a th c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles 5.2 Conclusion This method might have good result when apply to fluorescence spectrophotometer system, but when using HPLC system to determine that solution the result seem unstable and difficult to identify the real signal of ammonia So, it offers doubt about analysis capacity of that method in determine ammonia Therefore, to evaluate specific and accurate capability of NDA application, the experiment need more time for research the factor effect to value of ammonia in that method an lu n va p ie gh tn to d oa nl w oi m ll fu an v an lu nh at z z @ om l.c gm an Lu n va a th 46 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles REFERENCES Anne, M H & Constant, M G B (1993) Determination of ammonia in seawater using catalytic cathodic stripping voltammetry American Chemical Society, pp 3411–3416 Bill J C & Tarbell, D S (1954) o-Phthalaldehyde Organic Syntheses, Vol 4, pp 87 California Environmental Protection Agency (2011) Ammonia Retrieved from: http://www.waterboards.ca.gov/water_issues/programs/swamp/docs/cwt/guidance/3310en pdf (accessed on 17/06/2015) lu an Carlson, R G., Srinivasachar, K., Givens, R S & Matuszewski, B K (1986) New Derivatizing va Agents for Amino Acids and Peptides.1.Facile Synthesis of N-Substituted n gh tn to 1Cyanobenz[f]isoindoles and Their Spectroscopic Properties American Chemical Society, pp 3978-3983 p ie Press Clare, S d oa nl w Chisholm, H (1911) Ammonia Encyclopædia Britannica (11th ed.) Cambridge University (2015) Disadvantages Advantages of an HPLC Retrieved v an lu http://www.ehow.com/list_5911530_disadvantages-advantages-hplc.html (accessed on m ll fu an 13/06/2015) from: Erika, P F., & Arnaldo, A (2012) Cardoso A Method for Determination of Ammonia in Air oi nh using Oxalic Acid-Impregnated Cellulose Filters and Fluorimetric Detection, Journal of at z the Brazilian Chemical Society 23(1), pp 142- 147 z @ Irina, T , Ilnur, Kh , Mihail, K , Aleksey, M , & Andrey, B (2015) Automated procedure for gm l.c determination of ammonia in concrete with headspace single-drop micro-extraction by om stepwise injection spectrophotometric analysis Talanta , pp 34-37 an Lu n va a th 47 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles Kuo, C T., Wang, P Y., & Wu, C H (2005) Fluorometric determination of ammonium ion by ion chromatography using postcolumn derivatization with o-phthaldialdehyde Journal of Chromatography A, pp 91-97 Laura, A.A., Ginny, S.M, & Ronald, E.S (1984) The o-phthalaldehyde derivatives of amines for high-speed liquid chromatography.electrochemistry American Chemical Society, 56 (7), pp 1089–1096 Mark, C R & Marlin, D H (1987) Determination of Amino Acids at Subfemtomole Levels by High-Performance Liquid Chromatography with Laser-Induced Fluorescence Detection an lu American Chemical Society, pp 411-415 n va Monica, Z B (2006) Ion Chromatography Retrieved from: gh tn to http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html (accessed p ie on 7/08/2015) w Petr, Z (2004) Reactions of Orthophthalaldehyde with Nucleophiles American Chemical Society, d oa nl pp 3217–3238 v an lu Pierre de, M , John F S , Richard, S G , Robert, G C , & Takeru, H (1987) Naphthalene-2,3 dicarboxaldehyde/Cyanide Ion: A Rationally Designed Fluorogenic Reagent for Primary fu an Amines American Chemical Society, pp 1096 - 1101 m ll oi Thomas, F.G & Henze, G (2001) Introduction Voltammetry analysis theory and practice nh at Retrieved z z from:https://books.google.com.vn/books?id=yoFKXm0ssIC&pg=PA60&dq=voltammetry @ gm +%C3%A2nlysis&hl=en&sa=X&ved=0CCoQh3QFg2M#v=onepage&q&f=false om l.c (accessed on 19/07/2015) an Lu n va a th 48 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles Weiss, J (2004) Ion chromatography Retrieved from: https://books.google.com.vn/books?hl=en&lr=&id=PP2&dq=ion+chromatography+book &ots=6N6rHEh4Mi&sig &redir_esc=y#v=onepage&q&f=false (accessed on 7/08/2015) Zaifang, Z., Joann, J L., M Inês G S A , Qiaosheng P., Spas D K & Shaorong L A (2015) microfabricated electroosmotic pump coupled to a gas-diffusion microchip for flow injection analysis of ammonia Microchimica Acta, pp 1063-1070 Zhaolai, D , Zhenlong, W , Sichao, J & Guoyao, W (2014) Analysis of amino acid composition in proteins of animal tissues and foods as pre-column o-phthaldialdehyde an lu derivatives by HPLC with fluorescence detection Journal of Chromatography B, pp 116 - va n 127 gh tn to Zotou, A & Notou, M (2012) Study of the naphthalene-2,3-dicarboxaldehyde pre-column p ie derivatization of biogenic mono- and diamines in mixture and fluorescence-HPLC w determination Euroanalysis XVI, pp 1039–1048 d oa nl oi m ll fu an v an lu nh at z z @ om l.c gm an Lu n va a th 49 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles APPENDICES an lu n va p ie gh tn to Fluorescence spectrophotometer machine d oa nl w oi m ll fu an v an lu nh at z z @ om l.c gm HPLC fluorescence machine an Lu n va a th 50 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles an lu n va p ie gh tn to Solvent of HPLC system d oa nl w oi m ll fu an v an lu nh at z z Warm up system @ om l.c gm an Lu n va a th 51 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles an lu n va Stored Working Solution with Ice p ie gh tn to d oa nl w oi m ll fu an v an lu nh at z z @ gm Borate buffer working solution om l.c NDA working solution an Lu n va a th 52 c si (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles (Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles(Luận.văn).determination.of.ammonia.in.water.samples.using.nda.fluorescence.derivatization.with.different.nucleophiles