Plant growth promoting activities of potential streptomyces sp strains

VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY -  - GRADUATION THESIS Title: “PLANT GROWTH-PROMOTING ACTIVITIES OF POTENTIAL Streptomyces sp STRAINS” Hanoi - 2022 VIETNAM NATIONAL UNIVERSITY OF AGRICULTURE FACULTY OF BIOTECHNOLOGY -  - GRADUATION THESIS Title: “PLANT GROWTH-PROMOTING ACTIVITIES OF POTENTIAL Streptomyces sp STRAINS” Student : Tong Thi Mai Anh Class : K63CNSHE Faculty : Biotechnology Advisor : Dr Dang Thi Thanh Tam Hanoi - 2022 ACKNOWLEDGEMENTS First, I would like to express my deep and sincere gratitude to Dr Dang Thi Thanh Tam, Department of Plant Biotechnology, Faculty of Biotechnology, Vietnam National University of Agriculture enthusiastically guided her to complete this graduation thesis She imparted a lot of valuable knowledge during the course of the graduation thesis, creating all conditions for facilities and equipment to help me complete the research topic well I would like to express my sincere thanks to all my colleagues and friends at the Department of Biotechnology, Vietnam National University of Agriculture, for their support and guidance so that I can complete this graduation thesis Finally, I would like to thank my family, relatives, and friends for always helping and supporting me during my study and graduation thesis Hanoi, December 2022 Student Tong Thi Mai Anh i DECLARATION I declare that this is my research work All data and results in the thesis are honest and not duplicated or copied from another topic I certify that the information contained in the thesis is cited as the original Hanoi, December 2022 Student Tong Thi Mai Anh ii TABLE OF CONTENTS ACKNOWLEDGEMENTS i DECLARATION ii TABLE OF CONTENTS iii LIST OF TABLES v LIST OF FIGURE vi ABSTRACT vii PART I GENERAL INTRODUCTION 1 Background Aims and requirements PART II LITERATURE REVIEW 2.1.Introduction 2.2.Taxonomy 2.3.Morphology and growth cycle 2.4.Characteristics 2.4.1 Antibiotics 2.4.2.Antifungal action 12 2.5.Plant–Streptomyces interaction 14 2.5.1.Biofertilization effects 15 2.5.2.Biostimulation effects 15 2.5.3.Bioprotection 16 PART III MATERIALS AND METHODS 17 3.1.Materials 17 3.1.1 Culture media 17 3.1.2 Streptomyces strains 17 3.1.3 Banana cultivar 18 iii 3.1.5 Research location 18 3.2.Methods 18 3.2.1 Pot experiment plantlets 18 3.2.2 Preparation of fermentation Streptomyces sp strains broth 18 3.2.3 Preparation of Fusarium oxysporum f sp suspension 19 3.2.4 Pot experiments 19 PART IV RESULTS AND DISCUSSION 21 4.1.Studying the effect of two Streptomyces sp strains on the growth and development of pumpkin seedlings 21 4.2.Studying the effect of Streptomyces strains on the growth and development of banana plants 25 4.3.Studying the effects of Streptomyces strains in the soil condition with Fusarium oxysporum sp 28 PART V CONCLUSION AND PROPOSAL 32 5.1.Conclusion 32 5.2.Proposal 32 REFERENCES 33 iv LIST OF TABLES Table 2.1 List of some antibiotics produced by Streptomyces sp 10 Table 2.2 List of literature dealing with the antagonistic activities of Streptomyces spp against Fusarium species in vitro 12 Table 4.1 The effect of Streptomyces sp strains on the growth and development of pumpkin seedlings after 14 days of treatment 22 Table 4.2 The effect of two Streptomyces sp strains on the morphology of root 23 Table 4.3 The effect of Streptomyces sp strains on the growth and development of banana plantlets 25 Table 4.4 The effect of Streptomyces sp strains on pumpkin seedlings in the soil condition with Fusarium oxysporum sp after 28 days of treatment 29 Table 4.5.The effect of Streptomyces sp strains on pumpkin roots after 28 days of treatment 30 v LIST OF FIGURE Figure 2.1 Life cycle of Streptomyces sp modified Figure 4.1.The effect of biological treatment on the growth and development of pumpkin seedlings after 10 days 22 Figure 4.2 The effect of Streptomyces sp strains on the growth and development of banana plants 26 Figure 4.3 The effect of Streptomyces sp strains on the leaf area of banana plants 27 vi ABSTRACT This study, there consists of three treatments: Evaluating the effect of two Streptomyces sp strains on the growth and development of pumpkin seedlings, testing the effect of two Streptomyces sp strains on the growth and development of banana plantlets, and studying the effects of two Streptomyces strains in the soil condition with Fusarium oxysporum sp The result showed that two Streptomyces sp strains significantly stimulated the growth and development of pumpkin seedlings and banana plantlets The Streptomyces sp strains had a strong antagonistic ability against Fusarium oxysporum f sp by pot experiments From the results, it is obvious that Streptomyces sp strains are promising candidates to stimulate the growth and development of plants, and they can be used as biofertilizers in crop production vii PART I GENERAL INTRODUCTION Background Streptomycetes are Gram-positive bacteria that belong to the order Actinomycetales and the family Streptomycetaceae; there are over 800 different streptomycetes species (Donald et al., 2022) Streptomycetes are fairly widely distributed in nature and can be found in surface waters, and soils with a wide range of chemistry and structure, and as true endophytes or rhizosphere colonizers in plants(Vurukonda et al., 2018) They invade the internal organs of plants, namely the root system and the xylem tissues of the stem, as endophytic microbes, with no obvious changes to the morphology or physiology of their hosts They frequently significantly impact the cycling of nutrients in various natural ecosystems Due to their capacity to produce a wide range of secondary metabolites, which are crucial for clinical and biotechnological purposes, they may also significantly impact the population structure of ambient microbial communities (Nonthakaew et al., 2022) Streptomyces is crucial in the stimulation of plant growth, the promotion of plant health, the breakdown of organic wastes, and the synthesis of secondary metabolites, which have commercial value in the domains of agriculture and medicine (Narayanan & Glick, 2022) Streptomyces, which are found in the rhizosphere and rhizoplane, aid in the biocontrol of insect pests and plant pathogens as well as the enhancement of shoot and root growth, grain and stover yield, and biological nitrogen fixation (Gopalakrishnan et al., 2020) More than a third of the commercially available antibiotics are produced by streptomyces, which is remarkable for its intricate developmental cycle and generation of bioactive secondary metabolites Streptomyces’ secondary metabolism has produced subantibacterial, antifungal, antiparasitic, and immunosuppressive substances besides, Streptomycetes has just recently been taken into consideration as a compared with the non-inoculated seedlings (p