Effect of material treatment methods on recovery yield, quality parameters and antimicrobial activities of essential oil obtained from turmeric leaf using hydrodistillation

MINISTRY OF EDUCATION AND TRAINING HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION THESIS FOOD TECHNOLOGY EFFECT OF MATERIAL TREATMENT METHODS ON RECOVERY YIELD, QUALITY PARAMETERS AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OIL OBTAINED FROM TURMERIC LEAF USING HYDRODISTILLATION ADVISOR: HOANG VAN CHUYEN STUDENT: NGUYEN THI QUYNH HUONG LE THI CAM NHUNG SKL 0 Ho Chi Minh City, August, 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code: 2022-18116019 EFFECT OF MATERIAL TREATMENT METHODS ON RECOVERY YIELD, QUALITY PARAMETERS AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OIL OBTAINED FROM TURMERIC LEAF USING HYDRODISTILLATION NGUYEN THI QUYNH HUONG Student ID: 18116019 LE THI CAM NHUNG Student ID: 18116026 Major: FOOD TECHNOLOGY ADVISOR: HOANG VAN CHUYEN, PhD Ho Chi Minh City, August 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code: 2022-18116019 EFFECT OF MATERIAL TREATMENT METHODS ON RECOVERY YIELD, QUALITY PARAMETERS AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OIL OBTAINED FROM TURMERIC LEAF USING HYDRODISTILLATION NGUYEN THI QUYNH HUONG Student ID: 18116019 LE THI CAM NHUNG Student ID: 18116026 Major: FOOD TECHNOLOGY ADVISOR: HOANG VAN CHUYEN, PhD Ho Chi Minh City, August 2022 ii ACKNOWLEDGEMENTS We would like to express our very great appreciation to all of the lectures at Ho Chi Minh City University of Technology and Education in general and lecturers at the Faculty of Chemical and Food Technology especially We gained valuable knowledge and skills regarding Food Technology throughout our four years at the school This paper and the research behind it would not have been possible without the exceptional support of my supervisor, Dr Hoang Van Chuyen - lecturer at the Department of Food Technology, Faculty of Chemical and Food Technology, Ho Chi Minh University of Technology and Education From the initial concept to the final graduation thesis, he was always enthusiastic in his support, mentoring, and imparting knowledge and experience We are truly grateful, and we will remember everything forever A special thanks goes out to consultants Ho Thi Thu Trang and Nguyen Thi My Le, who are in charge of the Department of Food Technology laboratories and Chemical Laboratories respectively Their cooperation and assistance with tools, machines, and equipment was greatly appreciated during the course of our investigation Thanks to the professors in Food Technology Workshop 1, Microbiology Laboratory, Biochemistry Laboratory, and the Sensory Lab for providing a conducive environment in which to accomplish our thesis, we are also expressing sincere gratitude to them Finally, we would like to thank our family and friends, who have supported us throughout the process of making this thesis Since our professional knowledge and practical experience are still limited, the report inevitably has shortcomings, we look forward to receiving your comments to make this report more complete Thank you sincerely! iii DISCLAIMER We hereby declare that all the content presented in the graduation thesis was written by us with the guidance Ph.D Hoang Van Chuyen We also hereby certify that the contents referenced in the graduation thesis have been correctly and fully cited in accordance with regulations Ho Chi Minh City, August 9, 2022 Authors Nguyen Thi Quynh Huong Le Thi Cam Nhung iv 39 40 14.043 14.347 0.51 0.36 41 14.461 0.14 42 14.749 0.24 43 17.356 0.23 44 17.730 0.09 79 ha.,3.beta.,5.alpha.)]- (3E,5E)-2,6Dimethylocta-3,5,7-trien-2-ol Benzene, 2-ethyl-1,3-dimethylBenzene, 1-methyl-3-(1-methylethyl)2-Cyclohexen-1-one,4-hydroxy-3-methyl-6(1-methylethyl)-, transEthanone,1-(2-hydroxy-5-methylphe 25241 001450-72-2 35 nyl)Phenol, 2-methyl-5-(1-methylethyl) 2-Cyclohexen-1-one, 4-hydroxy-3-methyl6-(1-methylethyl)-, trans- 3-Methyl-4isopropylphenol 4-Hydroxy-3-methylacetophenone cis-.beta.-Farnesene (1S,5S,6R)-6-Methyl2-methylene-6- (4-methylpent-3-en-1yl)bicyclo[3 1.1]heptane (E)-.beta.-Famesene Tumerone (E)-.gamma.-Atlantone Curlone 2-Methyl-6-(4-methylenecyclohex2-en-1-yl)hept-2-en-4-one N-Ethyl-ptoluidine Appendix 10 Result of investigating sensory evaluation of 30 panelists No 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Hydro-distillation Color Aroma Overall 8 8 8 6 5 7 8 6 7 7 7 5 6 6 5 8 5 6 7 Color 7 6 5 5 7 6 7 7 Steam Aroma 8 7 7 7 6 Overall 8 7 9 6 9 7 80 Color 7 9 9 9 8 9 8 Microwave Aroma Overall 9 9 7 9 6 8 7 7 8 8 9 7 6 7 6 Color 7 7 7 6 6 5 6 Ultrasound Aroma Overall 9 8 8 6 8 9 9 7 7 9 7 8 27 28 29 30 5 7 7 5 9 8 8 81 7 6 8 9 8 9 Appendix 11 GC-MS chromatogram of turmeric leaves essential oil (no use treatment) 82 Appendix 12 GC-MS chromatogram of turmeric leaves essential oil (use Ultrasound treatment) 83 Appendix 13 Antimicrobial activity of essential oil from turmeric leaves (use ultrasound treatment) against S.aureus C (%) Incubation (days) 0.25 0.5 84 Appendix 14 Antimicrobial activity of essential oil from turmeric leaves (use microwave treatment) against S.aureus C (%) Incubation (days) 0.25 0.5 85 Appendix 15 Antimicrobial activity of essential oil from turmeric leaves (use steam treatment) against S.aureus C (%) Incubation (days) 0.25 0.5 86 Appendix 16 Antimicrobial activity of essential oil from turmeric leaves (no use treatment) against S.aureus C (%) Incubation (days) 0.25 0.5 87 Appendix 17 Antimicrobial activity of essential oil from turmeric leaves (use ultrasound treatment) against E.coli C (%) Incubation (days) 0.25 0.5 88 Appendix 18 Antimicrobial activity of essential oil from turmeric leaves (use microwave treatment) against E.coli C (%) Incubation (days) 0.25 0.5 89 Appendix 19 Antimicrobial activity of essential oil from turmeric leaves (use steam treatment) against E.coli C (%) Incubation (days) 0.25 0.5 90 Appendix 20 Antimicrobial activity of essential oil from turmeric leaves (no use treatment) against E.coli C (%) Incubation (days) 0.25 0.5 91 Appendix 21 Experimental data and fitted curve of turmeric essential oil (from different material treatment methods) for E.coli Appendix 22 Experimental data and fitted curve of turmeric essential oil (from different material treatment methods) for S.aureus 92 S K L 0