NguyenHoangTrungHieu TV pdf The University of Kitakyushu Faculty of Environmental Engineering Department of Life and Environment Engineering *** Indole 3 acetic acid induces an oxidative burst and an[.]
The University of Kitakyushu Faculty of Environmental Engineering Department of Life and Environment Engineering *** Indole-3-acetic acid induces an oxidative burst and an increase in cytosolic calcium ion concentration in suspension-cultured transgenic cell-lines of rice (Oryza sativa L.) Professor: Tomonori Kawano Student: Nguyen Hoang Trung Hieu 2012MAB401 The University of Kitakyushu Faculty of Environmental Engineering Department of Life and Environment Engineering *** Indole-3-acetic acid induces an oxidative burst and an increase in cytosolic calcium ion concentration in suspension-cultured transgenic cell-lines of rice (Oryza sativa L.) Professor: Tomonori Kawano Student: Nguyen Hoang Trung Hieu 2012MAB401 TABLE OF CONTENTS Indole-3-acetic acid induces an oxidative burst and an increase in cytosolic calcium ion concentration in suspension-cultured transgenic cell-lines of rice (Oryza sativa L.) ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS AND DISCUSSION CONCLUSION FIGURES Acknowledgment Abbreviations References Appendix Indole-3-acetic acid induces an oxidative burst and an increase in cytosolic calcium ion concentration in suspension-cultured transgenic cell-lines of rice (Oryza sativa L.) ABSTRACT Application of indole-3-acetic acid (IAA) to the suspensioncultured cells of rice (Oryza sativa L.) results in immediate induction of transient bursts of reactive oxygen species (ROS), especially of superoxide (O2·-) and hydrogen peroxide (H2O2), which are further followed by a transient increase in cytosolic free calcium ion concentration ([Ca2+]c) O2· dependent chemiluminescence of Cypridina luciferin analog and H2O2-dependent chemiluminescence of luminol suggested that approximately 5% of total O2·- could be converted to H2O2 in the cell suspension The level of IAA-induced O2·- was lowered by the treatments with several ROS scavengers but not with an inhibitor of NADPH oxidase, suggesting the involvement of alternative ROS generating system The IAA-induced [Ca2+]c elevation was significantly inhibited by Ca2+ channel blockers and a Ca2+ chelator, indicating that extracellular Ca2+ is required for causing the increased in [Ca2+]c In the present study, we aimed to provide the evidence in support of the earliest actions of IAA in the course of auxin-stimulated signal transduction paths, by focusing on the induction of O2·- generation and the elevation of [Ca2+]c Lastly, the relationship between two of newly observed signaling events, namely, ROS generation and calcium movement, following cellular perception of IAA is discussed based on the results obtained here