IDENTIFICATION OF THE FREQUENCY OF DRUG RESISTANCE MUTATIONS OF HELICOBACTER PYLORI IN GASTRIC PATIENTS IN HAI DUONG PROVINCIAL GENERAL HOSPITAL Author Le Thi Phuong Hai Duong Medical Technical Univer[.]
IDENTIFICATION OF THE FREQUENCY OF DRUG RESISTANCE MUTATIONS OF HELICOBACTER PYLORI IN GASTRIC PATIENTS IN HAI DUONG PROVINCIAL GENERAL HOSPITAL Author: Le Thi Phuong Hai Duong Medical Technical University; phuongsinh@ymail.com Abstract: In this study, 131 gastric biopsies were taken from patients with gastritis who came to Hai Duong Provincial General Hospital for examination and treatment by request from January 2015 to July 2015 and they met the criteria: i) aged from 18-80; ii) infected with chronic gastritis, ulcer or gastric cancer; iii) not using antibiotic for a month before endoscopy These patients were taken for endoscopy to have endoscopic biopsy samples at the antrum, body and edge of the gastric ulcer, organizations suspected of contracting stomach cancer These DNA gastric biopsies were extracted and the 23S rRNA gene was amplified to detect Helicobacter pylori (H.pylori) The 23S rRNA gene in patients with H.pylori positive was sequenced to determine mutation related to anti-drug of H.pylori The results have shown that 58 of 131 (44.2%) gastric patients were infected with Helicobacter pylori and 15 of 58 (25.8%) patients with Helicobacter pylori infection were found to have mutations Key words: Mutation; Helicobacter pylori; Drug Resistance; Frequency; Gastric Ulcer