BS EN 15587 2008 ICS 67 060 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BRITISH STANDARD Cereals and cereal products Determination of Besatz in wheat (Triticum aestivum L ),[.]
BRITISH STANDARD BS EN 15587:2008 +A1:2013 Incorporating BS EN 15587:2008 cIncorporating orrigendum corrigendum January 2009 January 2009 Cereals and cereal products Determination of Besatz in wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), rye (Secale cereale L.) and feed barley (Hordeum vulgare L.) ICS 67.060 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BS EN 15587:2008+A1:2013 National foreword This British Standard is the UK implementation of EN 15587:2008+A1:2013, incorporating corrigendum January 2009 It supersedes BS EN 15587:2008, which has been withdrawn The start and finish of text introduced or altered by amendment is indicated in the text by tags Tags indicating changes to CEN text carry the number of the CEN amendment For example, text altered by CEN amendment A1 is indicated by The start and finish of text introduced or altered by corrigendum is indicated in the text by tags Text altered by CEN corrigendum January 2009 is indicated in the text by The UK participation in its preparation was entrusted to Technical Committee AW/4, Cereals and pulses A list of organizations represented on this committee can be obtained on request to its secretary The publication does not purport to include all the necessary provisions of a contract Users are responsible for its correct application Compliance with a British Standard cannot confer immunity from legal obligations This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 December 2008 © The British Standards Institution 2013 Published by BSI Standards Limited 2013 ISBN 978 580 76413 Amendments/corrigenda issued since publication Date Comments 31 December 2009 Implementation of CEN corrigendum January 2009 31 October 2013 Implementation of CEN amendment A1:2013 EUROPEAN STANDARD EN 15587:2008+A1 NORME EUROPÉENNE EUROPÄISCHE NORM October 2013 ICS 67.060 Supersedes EN 15587:2008 English Version Cereals and cereal products - Determination of Besatz in wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), rye (Secale cereale L.) and feed barley (Hordeum vulgare L.) Céréales et produits céréaliers - Détermination du pourcentage d'impuretés dans le blé tendre (Triticum aestivum L.), le blé dur (Triticum durum Desf.), le seigle (Secale cereale L.) et l'orge fourragère (Hordeum vulgare L.) Getreide und Getreideerzeugnisse - Bestimmung von Besatz in Weizen (Triticum aestivum L.), Hartweizen (Triticum durum Desf.), Roggen (Secale cereale L.) und Futtergerste (Hordeum vulgare L.) This European Standard was approved by CEN on 21 June 2008 and includes Corrigendum issued by CEN on 14 January 2009 and Amendment approved by CEN on 24 August 2013 CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member This European Standard exists in three official versions (English, French, German) A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT É E UROPÉ E N DE NORMALISAT ION E UROPÄISCHE S KOMIT E E FÜR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels © 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members Ref No EN 15587:2008+A1:2013: E BS 15587:2008+A1:2013 EN 15587:2008+A1:2013 EN (E) EN 15587:2008+A1:2013 (E) Contents Foreword EN 15587:2008+A1:2013 (E) Page This document (EN 15587:2008+A1:2013) has been prepared by Technical Committee CEN/TC 338 “Cereals Foreword .3 and cereal products”, the secretariat of which is held by AFNOR Scope This European Standard shall be given the status of a national standard, either by publication of an identical Normative references .4 text or by endorsement, at the latest by April 2014, and conflicting national standards shall be withdrawn at the latest byTerms April 2014 and definitions 4 Principle Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights Apparatus Sampling This document supersedes EN 15587:2008 Procedure (see diagram in Annex B) This document includes Corrigendum issued by CEN on 2009-01-14 and Amendment approved by CEN Expression of results .9 on 2013-08-24 Precision .10 The start and finish of text introduced or altered by amendment is indicated in the text by tags !" 10 Test report 11 The modifications of the Schematic related CEN Corrigendum have been implemented at the appropriate places in the Annex A (informative) summary on Besatz 12 text and are indicated by the tags !" Annex B (normative) Diagram of procedure 13 According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following Annex C (informative) Example of calculation 14 countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Annex D (informative) Results of interlaboratory test 15 Republic, Denmark, Estonia, Finland, France, Former Yugoslav Republic of Macedonia, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Bibliography 26 Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Contents Contents Foreword BS 15587:2008+A1:2013 EN EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Page Page Foreword .3 This document (EN 15587:2008+A1:2013) has been prepared by Technical Committee CEN/TC 338 “Cereals Foreword Scope and cereal products”, the secretariat of which is held by AFNOR Scope Normative references .4 This European Standard shall be given the status of a national standard, either by publication of an identical Normative Terms and references definitions text or by endorsement, at the latest by April 2014, and conflicting national standards shall be withdrawn at the latest byTerms April 2014 and definitions 4 Principle Principle AttentionApparatus is drawn to the possibility that some of the elements of this document may be the subject of patent rights CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights Apparatus Sampling Sampling Procedure (see diagram in Annex B) This document supersedes EN 15587:2008 Procedure diagram in Annex B) 8 Expression(see of results This document includes Corrigendum issued by CEN on 2009-01-14 and Amendment approved by CEN Expression of results 9 Precision 10 on 2013-08-24 Precision 10 Test report 10 11 The start and finish of text introduced or altered by amendment is indicated in the text by tags !" 10 report Annex ATest (informative) Schematic summary on Besatz 11 12 The modifications of the related of CEN Corrigendum have been implemented at the appropriate places in the A Schematic summary on Besatz 12 Annex B (informative) (normative) Diagram procedure 13 text and are indicated by the tags !" Annex B C (normative) (informative)Diagram Exampleofofprocedure calculation 13 14 According to the CEN/CENELEC Internal Regulations, national standards organizations of the following Annex C Example calculation 14 D (informative) Results ofof interlaboratory testthe 15 countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Annex D (informative) Results of interlaboratory test 26 15 Bibliography Republic, Denmark, Estonia, Finland, France, Former Yugoslav Republic of Macedonia, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Bibliography 26 Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom 2 BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Scope This European Standard specifies the term Besatz (impurities) and describes methods for the determination of its components The term Besatz is used as a parameter for certain quality aspects in common wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), rye (Secale cereale L.) and feed barley (Hordeum vulgare L.) Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application For dated references, only the edition cited applies For undated references, the latest edition of the referenced document (including any amendments) applies EN ISO 6644, Flowing cereals and milled cereal products — Automatic sampling by mechanical means (ISO 6644:2002) EN ISO 13690, Cereals, pulses and milled products — Sampling of static batches (ISO 13690:1999) prEN ISO 24333, Cereals and cereal products — Sampling (ISO/DIS 24333:2006) ISO 5223, Test sieves for cereals Terms and definitions For the purposes of this document, the following terms and definitions apply 3.1 Besatz all matter of a sample of grain other than the basic cereal of unimpaired quality NOTE It comprises the four fractions: broken grains, grain impurities, sprouted grains and miscellaneous impurities NOTE Live pests are not considered as Besatz They are specified as a separate criterion NOTE A schematic summary on Besatz is given in Annex A NOTE Pictures of examples are given in [9] 3.2 broken grains all grains belonging to the basic cereal not damaged by pests and whose endosperm is partially uncovered, including grains damaged by threshing and grains from which the germ has been removed !3.3 grain impurities fraction consisting of shrivelled grains, other cereals, grains damaged by pests, grains in which the germ is discoloured (only for common and durum wheat), mottled grains (only for durum wheat) and grains overheated during drying Note to entry: For common wheat, grains in which the germ is discoloured are disregarded up to %." BS 15587:2008+A1:2013 EN EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) 3.3.1 shrivelled grains grains which after elimination of all other matter defined in this European standard pass through 20 mm slotted sieves with widths of the following dimensions: common wheat 2,0 mm durum wheat 1,9 mm rye 1,8 mm feed barley 2,2 mm feed barley with moisture content below 12,5% 2,0 mm and grains damaged by frost and unripe grains (green) NOTE Frost damaged kernels can show the following appearance: black or brown discoloration, bleached or blistered, distinctly wax-like or candied surface Frequently they have dark stripes showing through the sides of the kernel !3.3.2 other cereals all cereals grains, whatever their state, not belonging to the considered species Note to entry: In a durum wheat sample, all common wheat grains and their grain dockage components are classified as other cereals and vice versa 3.3.3 grains damaged by pests grains which show visible damage due to attack by insects, rodents, mites or other pests Note to entry: Grains attacked by wheat midge are considered unsound grains (3.5.2) only when more than half the surface of the back of the grain is coloured grey to black as a result of secondary cryptogamic attack Where discoloration covers less than half the surface of the back of the grain, they are classed as grains damaged by pests 3.3.4 grains with discoloured germ, mottled grains grains either a) with discoloured germ in which the tegument covering the germ is coloured brown to brownish black and in which the germ is normal and not sprouted; Note to entry: If the germ is coloured only with one little spot, the grain is considered sound Note to entry: This fraction only exists for common wheat and durum wheat Note to entry: For common wheat these grains are disregarded up to % or b) mottled which show a brown to brownish black discoloration elsewhere than on the germ itself, particularly in the crease Note to entry: If the coloration is only in the crease, grains are considered mottled only if the spot covers more than 1/4 of the length of the crease Note to entry: This fraction only exists for durum wheat." BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) 3.3.5 grains overheated during drying grains showing external signs of scorching but of which the kernels are sound NOTE Grains showing external signs of scorching and of which the kernels are coloured yellowish-grey to brownishblack are considered unsound 3.4 sprouted grains grains in which the radicle or plumule is clearly visible to the naked eye NOTE Account should be taken of the general appearance of the sample when its content of sprouted grains is assessed NOTE In some cereal classes the germ is protuberant, e.g in durum wheat, and the germ tegument splits when the batch of cereals is shaken These grains resemble sprouted grains but must not be included in that group Sprouted grains are only those where the germ has undergone clearly visible changes which make it easy to distinguish the sprouted grain from the normal grain !3.5 miscellaneous impurities (synonymous: Schwarzbesatz) fraction consisting of extraneous seeds, unsound grains, extraneous matter, husks, ergot, bunted grains and impurities of animal origin" 3.5.1 extraneous seeds seeds of plants, whether or not cultivated, other than cereals NOTE They comprise noxious and not noxious seeds Noxious seeds means seeds which are toxic to humans and animals This group also includes seeds hampering or complicating the cleaning and milling of cereals and seeds affecting the quality of products processed from cereals NOTE In some cases it may be necessary to distinguish between noxious seeds and not noxious seeds An indicative list of noxious seeds is given in Annex A of ISO 7970:2000 [4] !3.5.2 unsound grains grains of the species or another cereal rendered unfit for human consumption and, regarding feed grain, for feed consumption, owing to putrefaction, mildew, grains affected with fungi (such as fusarium), bacterial or other causes Note to entry: Unsound grains also include grains damaged by spontaneous heat generation or too extreme heating during drying which are fully grown grains in which the tegument is coloured greyish-brown to black while the crosssection of the kernel is coloured-yellowish grey to brownish-black Note to entry: Grains attacked by wheat midge are considered unsound grains only when more than half the surface of the back of the grain is coloured grey to black as a result of secondary cryptogamic attack Where discoloration covers less than half the surface of the back of the grain, they need to be classed with grains damaged by pests Note to entry: Grains affected with fusariosis are grains whose pericarp is contaminated with Fusarium mycelium; such grains look often slightly shrivelled, wrinkled and have pink or white diffuse patches with an ill-defined outline Grains affected by fusariosis are the major source of mycotoxins BS 15587:2008+A1:2013 EN EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) 3.5.3 extraneous matter all matter in a sample of cereals retained by a sieve with apertures of 3,55 mm and passing through a sieve with apertures of 1,0 mm, and stones, sand, fragments of straw and similar impurities in the sample which pass through a sieve with apertures of 3,55 mm and are retained by a sieve with apertures of 1,0 mm Note to entry: Other cereals and particularly large grains of the basic cereal and ergot which are retained by a sieve with apertures of 3,55 mm are not extraneous matter." 3.5.4 husks glumes and grain husks 3.5.5 ergot sclerotia of Claviceps purpurea 3.5.6 bunted grains grains filled with a fetid-smelling dust comprising the spores of smuts (Ustilago spp.) or brown-black spores of bunt (Tilletia spp.) 3.5.7 impurities of animal origin impurities originating from animals such as feathers, hairs, excrements, dead insects and fragment of insects Principle The principle of this method is to separate all the groups of Besatz, defined under 3.1, from the normal basic grains by sieving and manual selection Apparatus 5.1 Sample divider 5.2 Balance, capable to weighing to the nearest 0,01 g 5.3 Sieves machine fitted with a cover and a receiver, the slot-widths 3,5 mm × 20,0 mm, 2,2 mm × 20,0 mm, 2,0 mm × 20,0 mm, 1,9 mm × 20,0 mm, 1,8 mm × 20,0 mm, 1,0 mm × 20,0 mm, in accordance with the specification of ISO 5223 5.4 Sieving machine allowing a forceful horizontal backward and forward movement of the sieves, e.g 5.5 Magnifying glass, illuminated 5.6 Forceps or horn spatula 5.7 Pots, for retaining components vibrator base with mounted sieves, or a shaking sieve BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Sampling It is important the laboratory receive a sample which is truly representative and has not been damaged or changed during transport and storage Sampling is not part of the method specified in this International Standard Recommended sampling methods are given in EN ISO 6644, EN ISO 13690 and prEN ISO 24333 Procedure (see diagram in Annex B) Prepare by division a representative sample of around 250 g (a) and weigh it to the nearest 0,1 g This sample is sieved on a sieve column (one above with an aperture of 3,5 mm and the second under with an aperture of 1,0 mm) for 30 s either with a sieving machine (5.4), or by hand If sieving is performed by hand, it must consist of horizontal movements parallel to the length of the slots (around one backward and forward movement per second) The matter retained by the sieve with slotted perforations of 3,5 mm (5.3) and that passed through the sieves with slotted perforations of 1,0 mm (5.3), except other cereals and particularly large grains of the basic cereal and ergot, shall be weighed together and regarded as extraneous matter Stones, mud balls, straws, chaff and similar impurities from the overtails of the 1,0 mm slotted sieve (5.3) have to be picked out Both fractions are weighted and are regarded as extraneous matter (3.5.3) Impurities of animal origin should be counted, including those which passed through the sieve of 1,0 mm slotwidth (5.3) If necessary, a magnifying glass (5.5) should be used NOTE The count should be quoted separately in numbers per kg of common wheat, durum wheat, rye or feed barley as appropriate Remove ergot from the overtails of the 1,0 mm slotted sieve (5.3) and weigh to 0,01 g accurately The fraction retained is divided with the sample divider (5.1) to obtain a sub sample (c) between 50 g and 100 g Weigh it to the nearest 0,1 g This partial sample is spread out on a table and fractions defined in Clause are extracted by means of forceps or a horn spatula (5.6) In case multiple kinds of damages are observed, the damaged grain shall be added to the fraction with the highest importance for the overall quality In rye samples with respect of natural variability of the colour of rye grains, grains with discoloured germ or mottled grains (3.3.4) are not picked out If the partial sample contains unthreshed grains of the basic cereals, remove the grains from the glumes by hand and add them to the fraction husks (3.5.4) This sub sample without all the impurities is sieved for 30 s on a sieve (5.3) with an aperture of 2,0 mm for common wheat, 1,9 mm for durum wheat, 1,8 mm for rye, 2,2 mm for barley (2,0 mm if moisture content of barley is less than 12,5 %) All elements passing through are considered as shrivelled grains Weigh the Besatz-free overtails of the 1,0 mm sieve (d) and all the groups of Besatz to the nearest 0,01 g If, for a partial sample, the sum of broken grains (3.2), shrivelled grains (3.3.1), other cereals (3.3.2), grains damaged by pests (3.3.3), grains in which the germ is discoloured (3.3.4) grains overheated during drying (3.3.5), sprouted grains (3.4), extraneous seeds (3.5.1), unsound grains (3.5.2), husks (3.5.4), bunted grains (3.5.6) and the weight of the Besatz-free overtails of the 1,0 mm sieve (d) differs by more than 0,5 % from the sub sample weight (c), the determination is not valid and a new partial sample must be analysed BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Annex C (informative) Example of calculation An example of calculation of the Besatz fractions is given below: Symbol weight a sample weight xextraneous matter 250,0 [g] extraneous matter xergot c mass fraction 0,5 [g] 0,2 [%] ergot 0,15 [g] 0,06 [%] subsample weight 52,4 [g] Auxiliary calculation: a − x extraneous matter − x ergot a ⋅s × 100 = 250,0 − 0,5 − 0,15 × 100 = 1,9096 250,0 ⋅ 52,23 (C.1) x1 broken grains 1,00 [g] 1,9 [%] x2 shrivelled grains 2,57 [g] 4,9 [%] x3 other cereals 1,07 [g] 2,0 [%] x4 grains damaged by pest 0,90 [g] 1,7 [%] x5 discoloured germ 0,20 [g] 0,4 [%] x6 overheated during drying 0,25 [g] 0,5 [%] x7 sprouted grains 0,10 [g] 0,2 [%] x8 extraneous seeds 0,02 [g] 0,04 [%] x9 unsound grains 0,49 [g] 0,9 [%] x10 husks 0,09 [g] 0,2 [%] x11 bunted grains 0,01 [g] 0,0 [%] sum of Besatz fraction x1 to x11 6,70 [g] d weight of clean sample material 45,53 [g] s sum of Besatz fraction and clean sample 52,23 [g] 99,7 % of subsample 14 BS 15587:2008+A1:2013 EN EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Annex D (informative) Results of interlaboratory test An international interlaboratory test involving 15 laboratories in 11 countries was organized by the Federal Research Centre for Nutrition and Food, Institute for Cereal, Potato and Starch Technology (BFEL), Detmold (Germany) in March 2006 It was carried out on the following six samples: Sample Basic cereal Sample A: common wheat; Sample B common wheat; Sample C rye Sample D rye Sample E durum wheat Sample F durum wheat The results obtained were subjected to statistical analysis in accordance with ISO 5725-1 and ISO 5725-2 to give the precision data shown in Tables D.1 to D.14 NOTE The statistical evaluation of the results of the interlaboratory test was not possible for grains overheated during drying, smutty grains and impurities of animal origin Table D.1— Precision data for broken grains Samples A B C D E F 1,04 2,68 1,34 5,57 0,34 1,40 15 15 15 15 15 14 Repeatability standard deviation , sr , % w/w 0,24 0,32 0,23 0,55 0,16 0,27 Coefficient of variation of repeatability, CVr , % 23,4 11,9 17,2 9,9 47,4 19,2 Repeatability limit r (2,8 sr), % w/w 0,68 0,90 0,65 1,55 0,45 0,75 Reproducibility standard deviation , sR, % w/w 0,63 0,48 0,25 0,57 0,27 0,38 Coefficient of variation of reproducibility, CVR , % 60,8 17,9 18,9 10,2 80,3 27,2 Reproducibility limit R (2,8 sR), % w/w 1,77 1,34 0,71 1,58 0,77 1,06 Ratio r/R 0,38 0,67 0,91 0,98 0,59 0,71 Mean value, % w/w Number of laboratories after eliminating outliers, p 15 BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) ˜ ™ Key X = broken grain percentage Y = sr ♦ and sR Figure D.1 — Accuracy values versus mean values for broken grains Table D.2 — Precision data for shrivelled grains Samples A B C D E F 1,14 2,24 0,36 0,87 0,01 0,26 13 13 14 13 13 14 Repeatability standard deviation , sr , % w/w 0,12 0,28 0,11 0,19 0,01 0,09 Coefficient of variation of repeatability, CVr, % 10,8 12,3 30,8 21,5 72 34,2 Repeatability limit r (2,8 sr), % w/w 0,34 0,77 0,31 0,52 0,02 0,24 Reproducibility standard deviation, sR, % w/w 0,17 0,47 0,18 0,28 0,03 0,26 Coefficient of variation of reproducibility, CVR , % 15,3 20,8 51,5 32,4 228,8 102,7 Reproducibility limit R (2,8 sR), % w/w 0,49 1,31 0,52 0,79 0,07 0,74 Ratio r/R 0,70 0,59 0,60 0,66 0,31 0,33 Mean value, % w/w Number of laboratories after eliminating outliers, p 16 BS 15587:2008+A1:2013 EN EN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Table D.3 — Precision data for other cereals Samples A B C D E F 0,10 1,28 1,51 0,66 0,74 3,17 15 14 14 14 14 14 0,07 0,48 0,36 0,33 0,23 0,42 76 37,4 23,9 50 31 13,1 Repeatability limit r (2,8 sr), % w/w 0,21 1,34 1,01 0,93 0,64 1,16 Reproducibility standard deviation, sR, % w/w 0,08 0,70 0,43 0,35 0,32 0,76 Coefficient of variation of reproducibility, CVR, % 87,8 54,5 28,3 52,6 43,5 24 Reproducibility limit R (2,8 sR), % w/w 0,24 1,95 1,19 0,98 0,90 2,13 Ratio r/R 0,87 0,69 0,84 0,95 0,71 0,55 Mean value, % w/w Number of laboratories after eliminating outliers, p Repeatability standard deviation , sr , % w/w Coefficient of variation of repeatability, CVr, % Table D.4— Precision data for grains damaged by pests Samples A B C D E F 0,93 0,45 0,09 0,20 0,08 0,08 15 14 13 14 14 14 Repeatability standard deviation , sr , % w/w 0,29 0,15 0,03 0,10 0,09 0,09 Coefficient of variation of repeatability, CVr, % 31,4 33 39 51,2 117,8 108,1 Repeatability limit r (2,8 sr), % w/w 0,82 0,41 0,10 0,29 0,25 0,25 Reproducibility standard deviation, sR, % w/w 0,74 0,40 0,09 0,21 0,11 0,12 Coefficient of variation of reproducibility, CVR, % 79,4 88,3 105,9 101,4 146,3 142,7 Reproducibility limit R (2,8 sR), % w/w 2,08 1,11 0,26 0,58 0,31 0,33 Ratio r/R 0,40 0,37 0,37 0,50 0,81 0,76 Mean value, % w/w Number of laboratories after eliminating outliers, p 17 BS 15587:2008+A1:2013 ENEN 15587:2008+A1:2013 (E) EN 15587:2008+A1:2013 (E) Table D.5 — Precision data for grains in which the germ is discoloured, mottled grains1) Samples A B E F 0,07 0,43 6,49 5,53 14 15 15 14 Repeatability standard deviation , sr, % w/w 0,07 0,16 0,53 0,72 Coefficient of variation of repeatability, CVr, % 99,4 37,3 8,2 13 Repeatability limit r (2,8 sr), % w/w 0,19 0,44 1,48 2,01 Reproducibility standard deviation, sR, % w/w 0,13 0,29 6,31 4,59 Coefficient of variation of reproducibility, CVR, % 181,2 67,1 97,2 83,1 Reproducibility limit R (2,8 sR), % w/w 0,36 0,80 17,66 12,86 Ratio r/R 0,55 0,56 0,08 0,16 Mean value, % w/w Number of laboratories after eliminating outliers, p 1) The definition of this fraction is improved compared to the definition in the working document used for the interlaboratory test 18