980 e1 • eBOX 81 3 Postmortem Biochemical Genetic Evaluation To be performed on any deceased infant ,1 year old for which the cause of death is not apparent or any child with suspected IEM Analyses ar[.]
980.e1 • eBOX 81.3 Postmortem Biochemical Genetic Evaluation To be performed on any deceased infant ,1 year old for which the cause of death is not apparent or any child with suspected IEM Analyses are most reliable if obtained within hours after death Contact newborn screening laboratory for results of neonatal screening Obtain a 3-mm punch biopsy of skin or Achilles tendon for fibroblast culture A Prepare skin with chlorhexidine (Hibiclens) or alcohol Do not use Betadine because it may inhibit fibroblast growth B Use sterile technique C Store biopsy specimen in sterile RPMI culture media (if available) at room temperature May be stored in sterile nonbacteriostatic saline for up to 24 hours prior to culture if culture media are not readily available D Send to cytogenetics or biochemical genetics laboratory for culture, possible enzyme analyses, and frozen storage Collect blood via cardiac puncture (