Systemic inflammatory responses in patients with type 2 diabetes with chronic periodontitis

untitled Systemic inflammatory responses in patients with type 2 diabetes with chronic periodontitis Ruben Mesia,1 Fatemeh Gholami,1 Hong Huang,1 Michael Clare Salzler,2 Ikramuddin Aukhil,1 Shannon M[.]

Open Access Research Systemic inflammatory responses in patients with type diabetes with chronic periodontitis Ruben Mesia,1 Fatemeh Gholami,1 Hong Huang,1 Michael Clare-Salzler,2 Ikramuddin Aukhil,1 Shannon M Wallet,3 Luciana M Shaddox1,3 To cite: Mesia R, Gholami F, Huang H, et al Systemic inflammatory responses in patients with type diabetes with chronic periodontitis BMJ Open Diabetes Research and Care 2016;4:e000260 doi:10.1136/bmjdrc-2016000260 ABSTRACT Objective: The objective of this case–control study was to quantify the immune responsiveness in individuals with type diabetes (T2D) as compared with patients without diabetes (NT2D) diagnosed with periodontitis Research Design and Methods: Peripheral blood was collected from 20 patients with moderate-to-severe chronic periodontitis (10 T2D, 10 NT2D) Blood samples were stimulated with ultrapure Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) for 24 hours 14 cytokines/ chemokines were quantified in culture supernatants using multiplex technology Results: T2D individuals demonstrated higher unstimulated levels of interleukin (IL-6), IL-1β, tumor necrosis factor α, interferon γ, IL-10, IL-8, macrophage inflammatory protein 1α (MIP1α), and 1β (MIP1β), and higher stimulated levels of IL-6, IL-8, IL-10, MIP1α and MIP1β, along with lower unstimulated and stimulated levels of granulocyte-macrophage colonystimulating factor (GM-CSF) when compared with NT2D ( p0.05) Patients with T2D had higher body mass index (BMI) than NT2D ( p=0.0178) In the absence of LPS stimulation, peripheral blood cultures of individuals with T2D and chronic periodontitis presented with higher levels of IL-6, IL-1β, TNFα, INFy, IL-10, IL-8, MIP1α, and MIP1β compared with those from individuals without T2D but who still had chronic periodontitis (p