henipavirus mediated membrane fusion virus entry and targeted therapeutics

Viruses 2012, 4, 280-309; doi:10.3390/v4020280 OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Review Henipavirus Mediated Membrane Fusion, Virus Entry and Targeted Therapeutics Deborah L Steffen 1, Kai Xu 2, Dimitar B Nikolov and Christopher C Broder 1,* Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD 20814, USA; E-Mail: Deborah.Steffen@usuhs.mil Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA; E-Mails: xuk@mskcc.org (K.X.); nikolovd@mskcc.org (D.B.N.) * Author to whom correspondence should be addressed; E-Mail: cbroder@usuhs.mil; Tel.: +1-301-295-3401; Fax: +1-301-295-1545 Received: 30 December 2011; in revised form: 30 January 2012 / Accepted: 30 January 2012 / Published: 13 February 2012 Abstract: The Paramyxoviridae genus Henipavirus is presently represented by the type species Hendra and Nipah viruses which are both recently emerged zoonotic viral pathogens responsible for repeated outbreaks associated with high morbidity and mortality in Australia, Southeast Asia, India and Bangladesh These enveloped viruses bind and enter host target cells through the coordinated activities of their attachment (G) and class I fusion (F) envelope glycoproteins The henipavirus G glycoprotein interacts with host cellular B class ephrins, triggering conformational alterations in G that lead to the activation of the F glycoprotein, which facilitates the membrane fusion process Using the recently published structures of HeV-G and NiV-G and other paramyxovirus glycoproteins, we review the features of the henipavirus envelope glycoproteins that appear essential for mediating the viral fusion process, including receptor binding, G-F interaction, F activation, with an emphasis on G and the mutations that disrupt viral infectivity Finally, recent candidate therapeutics for henipavirus-mediated disease are summarized in light of their ability to inhibit HeV and NiV entry by targeting their G and F glycoproteins Keywords: paramyxovirus; entry; attachment glycoprotein; fusion glycoprotein; membrane fusion; receptor; ephrin-B2; ephrin-B3; inhibitor; antibody; immunotherapy Viruses 2012, 281 Introduction Hendra virus (HeV) and Nipah virus (NiV) are enveloped, negative sense single-stranded RNA viruses that were first identified in the mid 1990s following two separate outbreaks in Australia and Malaysia, respectively [1–3] Both outbreaks began as zoonotic respiratory infections in either horses (HeV) or swine (NiV) that were transmitted to humans in close contact with infected animals (reviewed in [4]) For both HeV and NiV the initial symptoms in infected humans manifest ~1 to weeks post infection with fever, chills, headache and myalgia but can progress to severe respiratory distress and/or acute encephalitis [1,5–10] Both NiV and HeV can also cause relapsed encephalitis in infected individuals several months to several years following recovery from acute infection [11–13] In nature both HeV and NiV are found in several pteropid fruit bat species (flying foxes) [14], but the routes of transmission to humans are different as HeV is transmitted from bats-to-horses and then to humans, while NiV transmission has been from bats-to-pigs and then to humans and also directly from bats-to-humans as well as humans-to-humans [15–18] (reviewed in [19]) Only sporadic outbreaks of HeV occurred in the years immediately following its initial recognition in 1994, but starting in 2006 annual outbreaks of HeV have occurred among the equine population in Australia, resulting in a total of seven human cases with four fatalities [8,20] However, in 2011 (June to October) an unprecedented number of eighteen independent outbreaks of HeV among horses in Australia was reported, culminating in the death or euthanasia of 23 horses, dog and the close monitoring of more than 60 individuals for HeV infection [21,22] Similarly, following the first outbreak in Malaysia and Singapore in 1998, almost annual outbreaks of NiV infection with high human case fatality rates (~70%) have been recorded primarily in Bangladesh but also India (reviewed in [23,24]) To date, there have been a total of 564 reported cases of NiV infection in people of which 299 have been fatal (reviewed in [23]) The repeated occurrences of henipavirus outbreaks among people and animals has demonstrated the importance for developing both intervention and countermeasure strategies to prevent cases of human infection [25–27] While outbreaks of HeV and NiV have been contained to Australia (HeV) and Malaysia, Bangladesh and India (NiV), additional serological and limited nucleic acid evidence suggests that antigenically related henipaviruses are circulating in other regions including Thailand, Indonesia, China, Madagascar and West Africa [28–34] Further serological evidence (cross-reactive antibodies to NiV glycoproteins) has also suggested that henipavirus transmission to domestic pigs in West Africa is possible [35] In addition to the apparent widespread occurrence of the henipaviruses in various bat species, and unlike all other paramyxoviruses, HeV and NiV possess a broad host tropism that includes pigs, horses, cats, dogs, guinea pigs, hamsters, ferrets, monkeys and humans, leading to the classification of HeV and NiV into the new genus henipavirus in the family Paramyxoviridae [36] Given the high morbidity and mortality rates associated with henipavirus infections in both humans and livestock, their recognized natural reservoirs in nature, the ease of propagation and a lack of any licensed vaccines or therapeutics, HeV and NiV pose significant biosecurity threats and are classified as biosafety level-4 (BSL-4) pathogens Virus attachment, membrane fusion and particle entry for HeV and NiV requires two distinct membrane-anchored glycoproteins: an attachment glycoprotein (G) and a fusion glycoprotein (F) The G glycoprotein is required for receptor binding and virion attachment to the host cell, and the F glycoprotein is directly involved in facilitating the merger of the viral and host cell membranes Viruses 2012, 282 As HeV-G and -F share a high degree of similarity with NiV-G and -F (approximately 83% and 89% amino acid identity for G and F, respectively), it also seems reasonable that the characteristics and features attributed to the viral glycoproteins of one virus may be representative of the corresponding viral glycoproteins of the other virus [37] Attachment Glycoprotein (G) Most of the well-described paramyxoviruses possess a multifunctional hemagglutinin–neuraminidase (HN) glycoprotein which binds the virions to sialic acid receptors on host cells, whereas some others, such as the morbilliviruses including measles virus (MeV), have an H attachment glycoprotein, which possesses only hemagglutinating activity, and uses the membrane proteins CD46 or CD150/SLAM as receptors, depending on the virus strain (reviewed in [38,39]) Recently, the adherens junction membrane protein nectin-4 on human epithelial cells has also been shown to be an important receptor for MeV [40,41] Like the HN and H glycoproteins, the henipavirus attachment G glycoprotein is a type II transmembrane protein that consists of an N-terminus cytoplasmic tail, a transmembrane domain, a stalk domain and a globular head; however the G glycoprotein possess neither hemagglutinin nor neuraminidase activities The globular head folds as a β-propeller with a central cavity surrounded by six blades, which themselves are composed of four anti-parallel beta sheets [42–44] The β-propeller shape is maintained by disulfide bonds between beta sheets in each blade as well as two additional disulfide bonds between blades three and four and between the N- and C-termini of the globular head Five potential N-linked glycosylation sites (N306, N378, N417, N481 and N529) have been identified in the globular head of NiV, and evidence has verified that four of the five sites are glycosylated with one site, N417, yielding variable reports likely owing to alternative expression methods [43–45] Likewise, the HeV-G head domain also has the same five predicted and conserved N-linked glycosylation sites occupied by carbohydrate moieties [46] Detailed glycan composition and site occupancy analysis of the entire ectodomain of HeV-G has recently been performed and has also revealed O-linked glycosylation sites in the protein [47] 2.1 Oligomerization of G Glycoprotein The native conformation of G when expressed on the virion or the surface of an infected cell is a tetramer, which is comprised of a dimer of dimers [44,48] Residues responsible for the oligomerization of G are isolated to the stalk domain as expression of the globular head alone results only in monomeric species [44] Further investigation determined that two disulfide bonds in the stalk domain of G enable dimer formation, but the specific interactions in the stalk domains between homodimers that enable G to form a tetramer are unknown [48] Bowden et al proposed that one surface of dimer-dimer interface occurs across the β1- and β6-propellers of the globular head [44,45] This suggestion is supported by the lack of both structural divergence and N-glycosylation sites, which would interfere with oligomerization, along this section of the protein Additionally, the recently reported structure and model of a tetrameric Newcastle disease virus (NDV) HN has provided further insight on the organization and oligomeric structure of a paramyxovirus attachment glycoprotein The stalk domains of NDV-HN form a four-helix bundle (4 HB) with a hydrophobic core that is the result of an 11-residue repeat domain in the stalk [49] Similarly to NDV-HN, HeV and NiV-G stalks contain Viruses 2012, 283 alpha helices with a predicted break from amino acids 95–98, and the modeled juxtaposition of these stalks with the globular heads of HeV-G resembles the tetrameric structure of NDV-HN (Figure 1) Figure Model of the Hendra virus attachment G glycoprotein The HeV-G ectodomain is shown in its dimer conformation The secondary structure elements of the two globular head domains, colored in green and blue, are derived from the crystal structure, which also revealed the five predicted N-linked glycosylation sites (N306, N378, N417, N481 and N529) occupied by carbohydrate moieties (gray spheres) [46,47] However, N378 was not modeled in the figure due to weak electron density The G glycoprotein head domain folds as a six-blade β-propeller with disulfide bonds illustrated as yellow sticks The residues of the ephrin-B2 G-H loop are shown in yellow While the entire structure of the HeV-G stalk domain (residues 71–173) has not been determined, residues 77–136 are modeled for each monomer suggesting this region forms a discontinuous helix (Helix Break) [50] The position of the HeV-G head dimer and stalks are oriented based on the alignment with the NDV structure and the receptor binding face of the blue monomer is facing out and the green monomer is facing left Despite having two helical ranges, Thr-77 to Lys-95 and Thr-98 to Ser-135, the HeV-G stalk residues 98–135 appear equivalent to the HN glycoprotein stalk helix domain of the recently reported NDV structure [49] Additionally, the Ile residues in the HeV-G stalk domain that can modulate conformational changes associated with receptor binding are indicated and are located in the alpha helical region of the HeV-G stalk domain that aligns with the NDV-HN stalk [51] Viruses 2012, 284 A model of a tetrameric parainfluenza virus (SV5) HN previously reported suggested a tetramer formation in which the globular heads of the two dimers were in contact [52], which contrasts with the more recent structural data of the NDV tetramer in which the globular heads of the dimers are separated [49] Although the globular head dimer of NDV and SV5 can be superimposed with a low 1.5 Å root mean square deviation (rmsd), the earlier SV5 tetramer model is not in accord with the recent NDV dimer and stalk configurations Given the characteristics of HeV and NiV-G, specifically the location of the N-linked glycosylation sites and the predicted stalk helices, it seems reasonable that the tetrameric form of the henipavirus G glycoprotein would also resemble the NDV-HN structural model (Figure 1) Receptors Ephrin-B2 and Ephrin-B3 The henipaviruses are the most recently recognized paramyxoviruses that also use host cell membrane proteins as virus entry receptors, and both HeV and NiV bind to ephrin-B2 and ephrin-B3 via their G glycoproteins [53–56] Human ephrin-B2 and ephrin-B3 are 39% identical in amino acid sequence and are members of a large family of surface expressed glycoprotein ligands that bind to Eph receptor tyrosine kinases and mediate bi-directional cell-cell signaling events within the nervous, skeletal and vascular systems [57,58] The ephrin-B2 and -B3 molecules are highly conserved proteins across species with amino acid identities ranging from 95–96% and 95–98%, respectively, including those hosts susceptible to henipavirus infection such as human, horse, pig, cat, dog and flying foxes [59] Ephrin-B2 is found in arteries, arterioles and capillaries in multiple organs and tissues including arterial smooth muscle and human bronchiolar epithelial cells but appears absent from venous components of the vasculature [60], whereas ephrin-B3 is found predominantly in the nervous system and the vasculature (reviewed in [61,62]) The identification of ephrin-B2 and ephrin-B3 as functional receptors for the henipaviruses in cultured cells provides some explanation for both the broad species tropisms of the viruses, owing to their highly conserved nature, and the observed distribution of viral antigen in arterial endothelial cells, smooth muscle, neurons, and some epithelial cells (reviewed in [63,64]) While it is unclear how many ephrin molecules are required to bind oligomeric G to activate the henipavirus membrane fusion process, recent structural data has revealed that a G glycoprotein head domain (monomeric) binds an ephrin molecule in a 1:1 ratio [42,43,46] Both ephrin-B2 and ephrin-B3 are able to support productive infection of HeV and NiV, but the binding affinities of HeV and NiV-G for ephrin-B2 and -B3 are uncertain and this is also complicated by the oligomeric nature of both the G glycoprotein and the ephrins One report has suggested HeV and NiV-G have the same binding affinity for ephrin-B3 while NiV-G has a higher affinity for ephrin-B2 than HeV-G; however another study indicated that HeV and NiV-G bound ephrin-B2 similarly while NiV-G engaged ephrin-B3 with a higher affinity in comparison to HeV-G [59,65,66] One possible explanation to explain these different findings is that two different HeV-G sequences were used Negrete et al determined that the sequences of two HeV-G strains currently used in research contain one amino acid change at position 507, having either a Ser or a Thr [66] A Thr at position 507 confers ephrin-B3 affinity similar to that of NiV-G, but a Ser at position 507 reduces the affinity of HeV-G for ephrin-B3 while having no affect on ephrin-B2 affinity Given the physiological locations of ephrin-B2 and -B3, the observed differences in Viruses 2012, 285 the transmissibility of HeV and NiV and the differences in HeV and NiV disease course in susceptible hosts upon infection, additional study of the henipavirus G glycoproteins and their interaction with the ephrin receptors will further our understanding of the biology and pathology of these important zoonotic agents 3.1 Interaction of Henipavirus G with Ephrin Receptors B-class ephrins contain a globular domain that is comprised of eight β-strands (identified as A-D, F-H and K) surrounding a hydrophobic core [67] Although there are three B-class ephrins (B1-B3) with high levels of similarity, only ephrin-B2 and ephrin-B3 are able to serve as functional receptors for HeV and NiV The major structural divergence between these ephrins occurs in the G-H loop, which is a 15 amino acid linker region between β-strands G and H that is also primarily responsible for the binding between ephrins and their cognate Eph receptors [68] Despite the 15-amino acid length of the G-H loop, only a short stretch of conserved amino acids (F/Y117/120SPNLW122/125) binds in the groove of the globular head of HeV and NiV-G with the only difference between ephrin-B2 and ephrin-B3 being F117 and Y120, respectively [42,43,68] (To avoid confusion, we will use the ephrin-B3 numerical designation for the G-H loop residues, identified as F/Y120-W125) Preceding the solution structures of the henipavirus G glycoproteins in complex with ephrin-B2 and -B3, the importance of the ephrin G-H loop was first hypothesized by Negrete et al and confirmed by mutagenesis that involved the conversion of two residues in the G-H loop of ephrin-B1 to the residues in ephrin-B2, making ephrin-B1 a functional NiV receptor [55] The ephrin-B1 amino acid replacements L124→Y and W125→M would result in steric clashes and converting these residues to match the ephrin-B3 sequence eliminates this hindrance [55] The overall conformation and flexibility of the ephrin-B2 and ephrin-B3 G-H loops might also influence receptor selectivity of the henipavirus G glycoproteins Indeed, both ephrin-B2 and -B3 appear to have G-H loops with extended and relatively rigid conformations, whereas ephrin-B1 has a more flexible G-H loop, which may not be compatible with the apparent lock-and-key ephrin/G glycoprotein binding mechanism [69] Binding between ephrin-B2 and HeV and NiV-G occurs in two regions that occlude approximately 2,700 Ǻ2 surface area on the interface [42,43] The first region occurs along a mostly polar area of the outer rim of the cavity in the globular head of the henipavirus G and requires 24 hydrogen bonds, four salt bridges and multiple hydrophobic interactions [42,43] The second region primarily involves interaction through van der Waals forces of the ephrin G-H loop residues F/Y120, P122, L124 and W125 with binding pockets in the central cavity of the globular head of G [42,43] The four binding pockets in HeV and NiV-G for the residues in the ephrin-B2 and -B3 G-H loop are highly conserved with only four differences, the most notable being a Val (NiV) to Thr (HeV) change at position 507 [42] (Table 1) The crystal structures of both HeV and NiV-G in complex with ephrin-B2 have provided much information on the specific interaction between these attachment glycoproteins and receptor, but whether relevant conformational changes must occur for G/ephrin-B2 binding is not completely clear It is generally accepted that the G-H loop of ephrin-B2 and -B3 does not undergo major conformational change, except for rearrangements of W125 upon G engagement that are suggested to “latch” ephrin-B2 into a stronger association [46] Viruses 2012, 286 Table Henipavirus G glycoprotein receptor binding pockets The residues in HeV-G and NiV-G that form the binding pockets for the residues of the ephrin-B2 and ephrin-B3 G-H loop are shown The residues are highly conserved with those in bold-face indicating the four amino acids that are different between the HeV-G and NiV-G binding pockets for Eprhin-B2 and -B3 P122, L124 and W125 Binding Pocket for loop residues F/Y P L W HeV-G glycoprotein residues C240, N557, A558, Q559, E579, I580, Y581, I588, R589 P488, G489, Q490, E505, G506, T507, Q530, T531, A532 Y458, W504, E505, G506 L305, V401, N402, W504 NiV-G glycoprotein residues C240, N557, A558, Q559, E579, I580, Y581, I588, R589 P488, G489, Q490, E505, G506, V507, Q530, T531, A532 F458, W504, E505, G506 L305, I401, R402, W504 While there is debate as to whether major or minor conformational changes occur in the monomeric henipavirus G head domain upon receptor binding, it is clear that some limited structural rearrangements occur but appear restricted to a specific region [42,43] Three of the binding pockets in the HeV and NiV-G head domain (pockets for ephrin-B2 and -B3 residues P122, L124 and W125) undergo little conformational change, while the G glycoprotein D579-P590 loop (binding pocket for ephrin-B2 and -B3 F117 and Y120, respectively) appears to have the greatest structural alteration upon receptor binding [44] Interestingly, this region is the one binding pocket that must accommodate two different residues, suggesting ephrin-B2 and -B3 bind to HeV and NiV-G by an induced-fit model as the F residue binding pockets in each henipavirus G glycoprotein must conform in a way to accommodate the different residues in the G-H loops of ephrin-B2 and ephrin-B3 [44,45] Other more recent data suggest that ephrin-B2 binding to HeV-G supports a “lock, key and latch” model for the association between the G glycoprotein and its receptors with the W122 residue of ephrin-B2 serving as the “latch” [46] Finally, it should also be recognized that the available structural data of a henipavirus G glycoprotein complexed with an ephrin receptor is from monomeric proteins and not take into account the possibility of broader oligomeric changes that may occur following receptor engagement For example, in case of MeV-H, it was recently reported that following receptor binding, the two heads of a MeV-H dimer move in relation to each other, and by stabilizing the H-dimer interface by inter-molecular disulfide bonds, fusion can be blocked, suggesting that oligomeric H conformational changes (dimer separation or movement) is linked to fusion triggering [70] 3.2 Mutations in the G Glycoprotein That Impact Function As might be expected, mutational studies have highlighted a number of residues in both the HeV and NiV-G glycoproteins and the ephrin-B2 and -B3 receptors that are critical for their interaction The majority of reported mutations in the G glycoprotein that affect function (i.e., the protein’s fusion promoting activity in the context of co-expressed F glycoprotein) are conserved between HeV and NiV and are located throughout the globular head and stalk domains Mutations located in the globular head that decreased fusion activation by at least 25%–50% can be separated into two groups based on their location in the globular head—distal or proximal to the ephrin binding site Group residues (D257A, D260A, K443A, G449A, K465A and D468A) are located closer to the stalk domain of the henipavirus Viruses 2012, 287 G glycoprotein, and are distant from the ephrin binding site, but despite this lack of proximity, all of these Group mutants display reduced binding to both ephrin-B2 and -B3 [56] Given the distance between the locations of these mutations and the receptor binding site, it is unlikely that the mechanism of reduced binding is due to steric hindrance/blocking of the binding site Bishop et al proposed that these mutations could act by locking HeV-G in a conformation that favors binding to HeV-F but not ephrin-B2 as these mutants demonstrated an increase in HeV-F co-association [56] A re-examination of some of these mutations in the context of the crystal structure of the dimeric interface of HeV-G provides some additional insight (Figure 2) As can be seen from the figure, mutants D257A and D260A appear to be involved in interaction between the two globular heads of the dimer, and disruption of the homodimer could lead to decreased receptor binding and resultant fusion activation (Figure 2A) Additionally, using the data reported by Yuan et al on the NDV-HN tetrameric structure [49], modeling the location of the HeV-G stalk domain to examine the impact of the Group mutants reveals that residues G449 and D468 appear to interact with the stalk domain Some conformational changes in G occur upon receptor binding, and therefore HeV-G would have pre-receptor-bound and post-receptorbound forms with structural differences in either the head or stalk (or both) domains The importance of the HeV-G stalk domain in the context of its fusion promoting activity and role in the maintenance of the protein’s conformation is discussed below (Section 5) Mutation of either G449 or D468 could destabilize the pre-receptor-bound structure of HeV-G and interfere with receptor binding and/or receptor-triggered conformational changes required for its fusion promoting activity (Figure 2B) The final two mutants in Group (K443A and K465A) are both located in a region of HeV-G that is part of a massive hydrogen bond network, which adds stability to the structure of HeV-G (Figure 2A) Mutation of these residues likely destabilizes this hydrogen bond network, preventing HeV-G from obtaining the correct conformation required for receptor binding and fusion See Table for a summary of mutant Groups The Group residues (N402A, Q490A, E501A, W504A, E505A, Q530A, T531A, A532K, E533Q, N557A, Y581A and I588A) are located on the outer surface of the globular head near the ephrin binding site and can be further broken down into sub-groups based on receptor usage in the context of either HeV or NiV-G [46,66,71] (Figure 2C and Table 2) The first sub-group (Group 2a) includes those residues that decreased fusion promoting activity by decreased receptor binding without knowing which ephrin receptor was used, and these mutations are Q530A, T531A, A532K and N557A [71] The second and third sub-groups (Subgroups 2b and 2c) contain mutations that decrease the fusion promoting activity of G upon either ephrin-B2 usage (N402A, E501A, E505A, G506A, E533A, Y581A and I588A) or ephrin-B3 usage (N402A, Q490A, E501A, W504A, E505A, G506A, E533A, Y581A and I588A) respectively [46] These two sub-groups of mutated residues are quite similar with only the additional Q490A and W504A mutations in Subgroup 2c that exhibit decreased fusion promoting activity in the context of HeV-G and ephrin-B3 interaction Interestingly, the residues of Subgroup 2b had no apparent decrease in ephrin-B2 binding but were significantly impaired in supporting fusion promoting activity as measured by pseudotyped virus entry, whereas the majority of mutations in Subgroups 2a and 2c had decreased receptor binding These findings were in good agreement with earlier observations that mutation of residues W504 and E505 impair ephrin-B3 binding and virus entry and mutation of E533 impairs both ephrin-B2 and -B3 usage [66] In the context of HeV-G mutations, E505A also revealed impaired ephrin-B2 usage by virus entry but little Viruses 2012, 288 effect on impaired binding, and mutation E533A, which abrogated both ephrin-B2 and -B3 usage in virus entry, had little effect on binding ephrin-B2 [46] Only two mutations in Subgroup 2c (N402A and I588A) possess significant impairment of HeV-G function but have no decrease in ephrin-B3 receptor binding Interestingly, among the Group mutants, only two of these residues (E501 and E533) are not directly involved in forming binding pockets for the G-H loop of ephrin-B2 or -B3 Figure Mutations in HeV-G that affect fusion promoting activity (A) Residues A257, A260, A443 and A465 that decrease HeV fusion when mutated are highlighted in red in the HeV-G globular head dimer Based on the location of residues A257 and A260, it is likely they are necessary for interaction between the two globular heads, while residues A443 and A465 are centrally located in the globular head in a region of extensive hydrogen bonding (B) The predicted structure of an HeV-G dimer with globular heads and stalk domains is shown with residues G449 and D468 highlighted in red These two residues are located in proximity to the stalk domain, and mutation of these residues decreases HeV fusion, suggesting they may be involved in interactions between the globular heads and stalk domains that are essential for the fusion process (C) Monomeric units of the globular head of HeV-G (gray) are shown in complex with the G-H loop residues FSPNLW of ephrin-B2 (yellow) Mutant Group residues that decrease fusion are located further from the ephrinB2 binding site and are shown in green Mutant Group residues that decrease fusion are shown in blue and cluster around the ephrinB2 binding site Residues that enhance fusion (Group e1) are shown in red and are also distally located from the ephrin-B2 binding site Given the divergent locations of the mutations that affect fusion, these mutations likely use different mechanisms, such as disrupting HeV-G structure or preventing ephrin-B2/B3 binding, to prevent HeV fusion Viruses 2012, 289 Figure Cont Table Summary of HeV-G Mutants that affect fusion Mutations that either decrease or increase HeV-mediated fusion are separated into various groups based on their location in the globular head (distal or proximal to ephrin-binding site) or stalk domain of HeV-G, which receptor is bound and whether the mutation affects receptor binding Information supplied in the Notes column is based on the modeled structures of HeV-G head and stalk domains or obtained from experimental data as indicated Squares shaded in gray represent information that is unknown or not relevant Effect Decrease Increase Location Globular Head— Distal Group Subgroup Residues Binds Notes D257, D260 Disrupts dimer Group G449, D468 Disrupts stalk-head interaction 1 K443, K465 Disrupts hydrogen bonding Q530, T531, Subgroup 2a Unknown Decreased receptor binding A532, N557 N402, E501, E505, G506, Subgroup 2b Ephrin-B2 No disruption in receptor binding E533, Y581, Globular Group I588 Head— Proximal Q490, E501, W504, E505, Decreased receptor binding G506, E533, Ephrin-B3 Subgroup 2c Y581 N402, I588 No disruption in receptor binding I101, I105, I112, Group Stalk I120, I124, I131, No disruption in receptor binding I138, I155, I160 D564, D470 No disruption in receptor binding Subgroup e1 Unknown G439 Decreased receptor binding V401, Q490, Globular Group No disruption in receptor binding Q559 Head e1 Subgroup e2 Ephrin-B2 W504 Decreased receptor binding Subgroup e3 V401, Q559 Ephrin-B3 No disruption in receptor binding Predictions based on modeled structure of HeV-G head and stalk domains; Experimental data Viruses 2012, 294 glycoproteins that with certain mutations decrease their receptor binding activity while strengthening the interactions with their respective F glycoprotein partner [56,105] Indirect support for the provocateur model also comes from data indicating that F expressed in the absence of G can be recognized by a conformation-dependent mouse mAb specific for the pre-fusion form of NiV-F [106] These conflicting data suggest that neither a clamp model nor a provocateur model alone can fully account for all the experimental observations to date on the mechanism of henipavirus-mediated fusion In fact, a recent report by Porotto et al details a new, third fusion mechanism that is based on both the clamp and provocateur models [107] Their new model is based on the need for continuous receptor engagement by hPIV3-HN to activate F for membrane fusion [107] In this study, fusion intermediates were captured by using HR2 peptides localized in the target cell membrane by a cholesterol tag It was determined that if interaction between hPIV3-HN and its receptor was interrupted during F activation and insertion into the target membrane, fusion would not occur However, no direct HN-F interactions were assessed, such as through a co-association or a co-immunoprecipitation approach Most recently, a further study by Porotto et al., examined the hPIV3 fusion system using a bimolecular fluorescence complementation approach to follow the dynamics of the viral HN and F in living cells [108] The authors were able to demonstrate that in this system the HN and F glycoproteins associate prior to receptor engagement, HN drives the formation of HN and F interacting clusters at the site of membrane fusion and the interaction of the HN-F pairs of oliogmers modulate the viral glycoprotein pair’s fusogenicity [108] This requirement of continual receptor engagement and interaction between the attachment and fusion glycoproteins was hypothesized to be applicable to all paramyxoviruses, including HeV and NiV [107] Further research regarding this proposed mechanism in respect to HeV/NiV may be able to resolve the conflicting data supporting the two current models and clarify the mechanism of henipavirus-mediated fusion Therapeutics Licensed and efficacious antiviral therapeutics for the henipaviruses are currently not available Ribavirin was used to treat 140 patients during the NiV outbreak in Malaysia in 1998/99, lessening the mortality rate by 35% from 54% in the control group to 32% in the treated group [109] Without any other currently available therapeutic options, ribavirin is still considered an option for treatment, but its impact on disease progression is questionable as two HeV infected patients in 2008 showed no discernable benefit after treatment with ribavirin [8] Additionally, chloroquine, an anti-malarial drug first demonstrated to block the proteolytic processing of HeV-F [110], was later shown to inhibit henipavirus infection in vitro [111] However, treatment with chloroquine and ribavirin proved ineffective for one HeV-infected individual in 2009 as no clinical benefit was observed [112] In subsequent animal challenge models with henipaviruses, ribavirin only delayed NiV disease and death and had no therapeutic effect against HeV infection in hamsters [113,114] Ribavirin also only delayed HeV disease by or days in African green monkeys and did not prevent disease outcome [115] Chloroquine, either alone or in combination with ribavirin, also had no therapeutic benefit in ferrets challenged with NiV or hamsters challenged with either NiV or HeV [113,116] Thus, due to the extreme pathogenic capacity of HeV and NiV infection in people, considerable effort has been spent in developing and exploring new therapeutic options against the henipaviruses, and these Viruses 2012, 295 treatments have primarily focused on targeting the fusion and entry step of the virus infection process and include F glycoprotein-targeted peptide fusion inhibitors and passive immunotherapy with virus neutralizing mAbs targeting the G and F glycoproteins Here we highlight the recent developments in therapeutics that target virus entry which have shown promise in treating henipavirus infection and pathogenesis 7.1 Peptide Fusion Inhibitors and Premature Fusion Triggering Heptad peptide based fusion inhibitors corresponding to HR1 or HR2 domains of a viral fusion protein have been shown to be particularly effective at inhibiting pH-independent Class I viral fusion systems in vitro, such as with paramyxoviruses, when present prior to triggering the fusion process leading to the formation of the HB (reviewed in [117]) Most of these approaches have utilized peptides corresponding to the HR2 domain, and they act by blocking HB formation by binding in the grooves of the HR1 trimeric core and prevent the viral HR2 domains from binding The first promising henipavirus-specific therapeutic was a 36 amino acid HR2-based fusion inhibitor (NiV-Fc2) [118] analogous to the approved HIV-1 specific therapeutic peptide enfuvirtide (Fuzeon™) Addition of exogenous NiV-Fc2 peptide could potently block HeV and NiV membrane fusion and live virus infection [118,119] The effectiveness of the NiV-Fc2 fusion inhibitor against henipavirus infection in vivo in a suitable animal model is presently being evaluated [120] While some approaches have focused on optimizing the length and sequence of exogenous HR2 peptides to increase their efficacy, one study determined that an HR2 peptide derived from hPIV3 proved more effective at inhibiting henipavirus mediated fusion than a peptide derived from HeV HR2 [121,122] The increased efficacy of the hPIV3 peptide appears to be due to stronger interactions between the hPIV3 HR peptide and the binding grooves of the HR1 domains than HR peptides corresponding to the native henipavirus F HR2 peptide sequence More recently, these observations were followed up with testing of a sequence-optimized and cholesterol tagged hPIV3-based HR2-derived peptide that targets hPIV3 and henipavirus F in the hamster-model of NiV infection [123] Here, these cholesterol-tagged peptides could also penetrate the CNS and exhibit some effective therapeutic activity against NiV infection in the hamster model; this peptide approach could be particularly effective in treating the encephalitic manifestations of henipavirus infection While there appears to be potential for peptide fusion inhibitors to be highly effective in preventing infection, there remains numerous challenges to overcome in the design and administration of such a therapeutic protocol The peptide based fusion inhibitors should likely be small and soluble with a long half-time in the bloodstream, but they must still be able to specifically bind the heptad repeats in their target F glycoprotein in order to block HB formation and membrane fusion The time frame of effective use of peptide fusion inhibitors may also be narrow because these inhibitors are only effective if present prior to HB formation but after the fusion peptide has been inserted into the target cell membrane In addition, as with most antiviral therapeutics, peptide inhibitors would also face resistance problems since mutations may likely arise within the heptad repeat sequences of the F glycoprotein Despite these challenges, additional in vivo efficacy testing of peptide fusion inhibitors of henipavirus infection merits further investigation Viruses 2012, 296 Recognizing that conformational changes occur in both G and F upon receptor binding, it was suggested earlier from mutagenesis results on HeV-G that a fusion triggered conformation could be generated in the absence of receptor, and thereby render the F-G complex ineffective (discussed above) [51] Recently, Porotto et al demonstrated that protocells expressing ephrin-B2 were capable of inactivating NiV-F and -G expressing pseudovirus by premature fusion triggering [124] Similarly, it was demonstrated early on that soluble ephrin-B2 henipavirus receptor was capable of blocking fusion and virus entry, but its antiviral activity was presumed to be due to simply blocking virus-host cell engagement rather than also serving as a premature fusion trigger [53,54] Not surprisingly, it was also shown that recombinant soluble versions of the ephrin-B2 receptors (EphB4 and EphB2) could also block henipavirus-mediated membrane fusion by binding to ephrin-B2 on the host cell making it unavailable for G glycoprotein binding [54] Finally, the scenario of premature fusion triggering as a potential therapeutic approach was presented with the identification of a small molecule capable of binding hPIV3 HN and triggering F to undergo conformational change in the absence of receptor binding, again rendering this paramyxovirus glycoprotein pair ineffective and further suggesting that strategies aimed at premature fusion activation may be a viable and interesting antiviral strategy [125] 7.2 Monoclonal Antibodies For paramyxoviruses, antibodies specific for either the F or attachment glycoproteins can neutralize virus with antibodies directed against attachment glycoproteins typically being the more predominant (reviewed in [126]) The first evidence of passive protection against a NiV challenge was demonstrated using hamsters with monospecific polyclonal antiserums against F and G [127] Passive immune plasma therapy was also successful in the post-exposure treatment of African green monkeys infected with NiV [120] However, the development of virus neutralizing mAbs has made passive antibody therapy development a major focus of current research Another passive immunotherapy study in the hamster model using two murine mAbs against NiV-F and two against G was shown to completely protect the challenged animals if animals received mAbs before and immediately following challenge, and again, mAbs targeting the G glycoprotein proved more effective than mAbs targeting the F glycoprotein [128] Similar results were also obtained in a HeV challenge model in the hamster [129] A major advance in the development of specific mAbs has been the use of recombinant antibody technologies [130,131] Earlier, recombinant, soluble G (sG) glycoprotein from HeV was used to isolate human mAbs One particular human mAb (m102.4) was HeV and NiV cross-reactive and possessed extremely potent virus neutralizing activity [132,133] In vivo studies have since demonstrated that m102.4 can protect animals from a lethal challenge with henipaviruses as a post-exposure application in the ferret model with NiV [134] or HeV [135] Most recently, mAb m102.4 was tested in the African green monkey model against HeV, and again all animals could be protected from lethal disease by m102.4 when it was administered from 12 to as late as 72 hours after a lethal high dose intratracheal challenge [136] In August 2009, m102.4 was used on a compassionate basis to save the life of a HeV-infected individual while in a coma [137] Unfortunately, delivery and intravenous administration of only 100 mg of available antibody occurred after the onset of encephalitis and the individual died shortly thereafter However, during the 2010 HeV emergence, prior to HeV diagnosis or the onset of clinical disease, two individuals that were considered as high risk cases of possible Viruses 2012, 297 infection received m102.4 antibody at doses sufficient to achieve a high serum concentration, and both individuals have remained healthy [138] Together, these findings highlight the therapeutic potential of mAb-based passive transfer modalities for treating henipavirus exposure Presently, m102.4 is being developed further for clinical use in people The mechanism and efficacy of m102.4-mediated neutralization is likely due to its ability to directly compete with ephrin-B2 and -B3 receptors for binding to the HeV and NiV-G glycoprotein Indeed, this seems born out when a comparison of the G glycoprotein binding pockets used by m102.4 and ephrin-B2 and -B3 is made, which shows a remarkable series of identical contacting residues in G that are important for engaging the ephrins as well as mAb m102.4 (Figure 3) [139] Substituting for the G-H loop of ephrin-B2 and -B3, m102.4 has a stretch of amino acid residues (L105APHPS110) that bind the henipavirus G glycoprotein with four residues considered critical for binding (L105, P107, H108 and P109) Figure HeV-G residues that bind ephrin and monoclonal antibody m102.4 A monomeric unit of the globular head of HeV-G (gray) is shown with residues important for ephrin-B2 and -B3 binding highlighted in purple, residues required for binding mAb m102.4 shown in blue and the residues of the ephrin-B2 G-H loop in yellow While the conformations of the residues may be slightly different, almost every residue involved in binding ephrin-B2 and -B3 is also required for binding m102.4, suggesting m102.4 prevents HeV and NiV infection by preventing ephrin-B2 and -B3 binding Viruses 2012, 298 Concluding Remarks The henipaviruses present credible natural and potentially deliberate biothreats to human and livestock populations While our knowledge of the viral mechanisms involved in the infection process has increased there are still many unanswered questions Through mutational studies it has been shown that receptor binding alone to G is not sufficient to trigger the F glycoprotein-mediated membrane fusion process Rather, receptor engagement and membrane fusion appear to be two separate but connected steps as receptor engagement does appear to trigger conformational changes in G that likely lead to the activation of the fusion activity of F While the crystal structures of the henipavirus G glycoproteins both alone and in complex with receptor have helped to identify the critical residues for receptor binding as well as some of the conformational changes induced by receptor binding, further studies will be required to determine the overall conformational changes that occur in the native tetrameric structure that are also postulated to lead to activation of F In addition, the specific interactions between the henipavirus G and F glycoproteins, including those that are specifically required for F activation, still need to be clarified Adding further complexity to G and F interaction and the fusion mechanism is the recently proposed model of paramyxovirus fusion that suggests fusion activation involves receptor engagement for F activation but also continual F-interaction by the receptor-bound G glycoprotein [107] Whether the henipavirus membrane fusion process aligns more with the clamp, provocateur or continual engagement fusion model (or a combination of these) will require further clarification Finally and perhaps most importantly, the development of human mAbs that potently inhibit the glycoprotein-mediated entry of these viruses into cells has for the first time provided a safe and effective therapeutic strategy, which is currently being used for treatment of people in emergency situations Acknowledgments The authors wish to thank Dimiter S Dimitrov for critical review of the manuscript and helpful suggestions Supported in part by the Department of Health and Human Services, National Institutes of Health, grants AI054715 and AI077995 The views expressed in the manuscript are solely those of the authors, and they not represent official views or opinions of the Department of Defense or The Uniformed Services University Conflict of Interest C.C.B is a United States federal employee and coinventor on United States patents 7,988,971: Human monoclonal antibodies against Hendra and Nipah viruses; and 7,666,431 Compositions and methods for the inhibition of membrane fusion by paramyxoviruses; assignees are The United States of America as represented by the Department of Health and Human Services 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