... Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy2 Department of Biotechnology, University of Verona, Italy3 Department of Clinical and Biological Sciences, University of Insubria, ... b-gal and a-syn cells treated or not with dopamine, as describedabove. Statistical analysis, by two-way analysis of ββ-Actinα-SynucleinFig. 1. Relative expression of a-synuclein in b-gal and ... manually excised and destained(1 · 10 min 50 lLofK3[Fe(CN)6]30mm and Na2S2O3100 mm;6· 10 min 100 lL of deionized water; 1 · 20 min100 lLofNH4HCO3200 mm;1· 20 min 100 lL of deion-ized...