Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 1) docx
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 1) docx
... are
three basic types of molecular forces: ionic forces, polar forces, and dispersive
forces. Each specific technique capitalizes on each of these specific forces:
1. Polar forces are the dominant type ... employed
in normal-phase HPLC (see Chapter 5).
2. Dispersive forces are employed in reversed-phase HPLC (see
Chapter 4).
3. Ionic forces are employed in ion-exchange HPLC (see Chapter...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 8E) docx
... that was optimized by Drylab, a forced degrada-
tion sample was run that was stressed at 50°C for 1 week in pH 1 diluent. It
was noted that a major impurity was formed (Figure 8-49) and was determined
to ... isocratic conditions for deter-
mining the most suitable pH for the method. The retention behavior of active
as a function of pH (isocratic) was determined. The best mobile-phase pH...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 8F) docx
... crystals that will
damage the seal over time. Therefore it is recommended to wash the HPLC
system with acetonitrile/water (20:80) for at least 30min before the system is
shut down to remove any potential ... of analysis is critical
to the success for fast and effective method development. The general
approach for the method development for the separation of pharmaceutical
compound...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 3) docx
... practically applicable for HPLC support.
Chemical modification of the silica surface with hydridosilane-type modi-
fiers as shown in schematic (3- 11) offers some advantages:
(3- 11)
A byproduct of this ... in HPLC are porous particles with average
diameters between 3 and 10µm. For most pharmaceutical applications, 3-µm
particle sizes are recommended. Porosity provides the surface a...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 13) docx
... or microorganisms to screen their spectrum of
605
HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto
Copyright © 2007 by John Wiley & Sons, Inc.
baseline setup, ... treated as a whole
(for more details see www.fda.gov; General Principles of Software Validation;
Final Guidance for Industry and FDA Staff, January 11, 2002). The PQ is
usually perf...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 14) docx
... is highly unlikely that the synthetic process
641
HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto
Copyright © 2007 by John Wiley & Sons, Inc.
of blanks injected ... than ELSD [24].
HPLC DETECTORS 655
Electrochemical detection can be utilized for compounds that are ionic or
readily oxidizable or reducible
.Thus, this form of detection can be...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 22) docx
... the two sets of crystals, the molecules are
987
HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto
Copyright © 2007 by John Wiley & Sons, Inc.
mobile phases ... conformation with the N-TFA-α-amino acid isopropyl ester.
(Reprinted from reference 36, with permission.)
The introduction of the diamide derivatives for enantiomeric separation
was a ste...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 7A) pptx
... ions on the basis of mass-to-charge ratios,
281
HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto
Copyright © 2007 by John Wiley & Sons, Inc.
low-polarity ... the most widely used systems for analysis of large bio-
molecules. For a continuous ion source, the ions can be stored for a short
period of time and pulsed out for analysis. In the...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 7B) pptx
... metabolites are
important biotransformation pathways for many drugs and xenobiotics [127,
132, 133]. For example, the formation of acyl glucuronide conjugate forced the
withdrawal of four marketed ... intermediates
for formation of the keto acid, were also observed in the radiochromatogram
of rat plasma (spectrum not shown) [131].
PRACTICAL APPLICATIONS 335
Scheme 11. Mechanism for th...
Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 8A) ppt
... development; these include speed
347
HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto
Copyright © 2007 by John Wiley & Sons, Inc.
for these studies. Fast gradient ... the ACD prediction of the solubil-
ity for the ionized form of bases and acids shows greater deviation than for
the solubility predicted for the neutral form of the molecule...