Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... S ¯ adhan ¯ a Vol. 35, Part 4, August 2010, pp. 461–468. © Indian Academy of Sciences Design, fabrication, and characterization of a solenoid system to generate magnetic field for an ECR proton source SKJAIN ∗ ,PANAIKandPRHANNURKAR Raja ... field. the magnetic field can cause diffusion of the plasma particles to the wall of the plasma cham- ber...
Ngày tải lên: 22/12/2013, 08:58
... species was detected with g values at 2.031, 1.994, and 1.951. The resonance started to develop at potentials ‡ 0 mV and was stable at potentials up to +350 mV. The loss and formation of the resonance ... of AF499 was identified as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence ()...
Ngày tải lên: 21/02/2014, 03:20
... bands from SDS ⁄ PAGE was performed as described by Bamford et al. [22]. MS MALDI-TOF MS was performed using an Ultraflex TOF ⁄ TOF instrument (Bruker Daltonik, Bremen, Germany). Pro- teins and ... minimal med- ium [19] with 2% lactose as a carbon source, 1% potassium phthalate as a buffering agent (pH 5.5), 0.1 mm CuSO 4 and 1% tyrosine as an indicator substrate was used. The tra...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... concentrations less than 25 m M . However, N-acetyl derivatives, namely N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc) and N-acetyl mannosamine (ManNAc) were better inhibitors than their ... animal glycoconjugate. They act as an important component of the ligands recognized by the lectins. Recognition can be affected by specific structural variations and modifications of...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... intermediate of Fig. 5. Putative genomic organization of the human phosphohistidine phosphatase gene. Astandard BLAST search, with AF164795 as the query, was performed against the nonredundant databases ... PROCEDURES Materials Phosphoamidate was prepared according to the method of Wei and Matthews [4]. Suc-Ala-His-Pro-Phe-pNA was purchased from Bachem AG, Switzerland. Malachite gre...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... amount of Bip-Pro in the extracellular uptake medium was quantified according to the laboratory standard HPLC (La-Chrom Ò ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and a ... inves- tigation because of their physiological importance and their pharmaceutical relevance as drug carriers [1–6]. Both transporters catalyse the uptake of most dipep- tides and t...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... SmRXR1 can interact with mammalian coac- tivators of transcription, and S. mansoni coactivators of transcription may have a similar mechanism to SmNR1 ⁄ SmRXR1. Recently four NR coactivators, SmGCN5, ... 13 and 23 contain nonspecific competitor. Lanes 4, 14 and 24 contain DR0 as competitor. Lanes 5, 15 and 25 contain DR1 as competitor. Lanes 6, 16 and 26 contain DR2 as compet...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles doc
... treated with dithiothreitol (5 mm) to reduce the disulfides, and assayed as above. Thiols were quanti- fied by comparison of peak areas to standard curves of those standards. Coenzyme A was measured ... the ratio of protein concentration to flavin concentration (as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a significant level of identity to...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... Storz, G. & Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®c antioxidant from m ammalian brain: alkyl reductases and thiol- speci®c antioxidant de®ne a large family o f antioxidant ... membrane was exposed to X-ray ® lm at )20 °Cor, when using an intensifying s creen, at )80 °C. RNA for Northern analyses was isolated as described above and separated in a 1.3% ag...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... microorga- nisms, plants and animals and their function and physio- logical roles have been investigated and identified [12,13]. N-acyl- D -amidohydrolase (EC.3.5.1.81, N-D-AAase) is an enzyme capable ... SDS/PAGE and Western blotting of N-D-AAase. (A) SDS/ PAGE (10% acrylamide). M, Molecular mass standards; lane 1, crude extract; lane 2, heat treatment of crude extract; lane 3...
Ngày tải lên: 08/03/2014, 16:20