... TGase, the length of the cDNA was smaller than the length deduced from the molecular size of the purifiedprotein. Furthermore, an initiation codon was not observedin the sequence obtained. Therefore, ... 5¢-GGACAATTACAGATTCAGTGGGAAAG-3¢ (sense, 1815–1840) and 5¢-CGAGTATACGAAATCGATGTCGTAG-3¢ (anti sense, 1729–1753) under the same conditions. In the second 5¢ RACE, first-strand cDNA was synthesized withanother oligonucleotide ... primer, 5¢-CCCCTCCTAATAGCGAAGAA-3¢ (antisense, 692–711). The first PCR ampli - cation was performed with gene-specific primers: 5¢-GGTCATTCAGTCGATCGATTTAC-3¢ (sense, 616–638) and5¢-TGGAACAACTGGAACGGGTGCTG-3¢...