... mRNA Purification Kit (AmershamBioscience) was used for isolation of mRNA from P. hilarislarval midguts. First-strand cDNA synthesis from the isolated mRNA and the following amplification of the target ... endo-b-1,4-glucanase and b-glucosidase, have been detected in the gut of P. hilaris larvae and adults [8].To clarify further the cellulase activity in P. hilaris,wepurified, characterized and obtained the ... Purification, characterization, cDNA cloning and nucleotide sequencingof a cellulase from the yellow-spotted longicorn beetle,PsacotheahilarisMasahiro Sugimura1, Hirofumi Watanabe1, Nathan...