Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... as α, β and γ (4). In this study, we also examine the effects of PKA on claudin-1 expression and localization, and how the localization of claudin-1 can affect melanoma cell mo-tility. Significance ... modifications of the tight junction protein claudin-1 cause its translocation to the cytoplasm and nucleus and that the subcellular localizatio...
Ngày tải lên: 03/11/2012, 11:17
... protein, indicating that His273 played an essential role in the activity of the enzyme, most likely as the residue phosphorylated by ATP. Phosphorylation of Cl -ACL In order to investigate the reaction ... reaction mixture. The results are shown in Table 1. The enzyme exhibited maximum activity in the presence of ATP, and in the presence of dATP showed 40...
Ngày tải lên: 08/03/2014, 22:20
... for the conformational stability of the protein and its proper interaction with the membrane protein-sorting machinery. The importance of helix 1 in stabilizing the UGT protein may also be inferred from ... the elongation of carbohydrate chains by appropriate glyco- syltransf erase s. Changes in the activities of NSTs may affect the structure of oligosacchari...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo Y học: Thermolysin-linearized microcin J25 retains the structured core of the native macrocyclic peptide and displays antimicrobial activity doc
... to be maintained in the thermolysin-linearized form. In the cyclic MccJ25, the stability is likely to be reinforced by the constraining strength of the circular backbone. Interestingly, the stable ... 268, 2124–2133]. The molecular mechanisms involved in the biosynthesis, folding and mode of action of MccJ25 are still unknown. We have investigated the structure...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo y học: "Excess circulating angiopoietin-2 is a strong predictor of mortality in critically ill medical patients"
... was informed of thestudy purpose and consent was required to further maintainstatus as study participant. The study was performed inaccordance with the declaration of Helsinki and approved bythe ... domain of the Tie2 receptor,which is almost exclusively expressed by endothelial cells.Binding of the agonist Ang-1 to the Tie2 receptor promotesvessel integrity, inhibits va...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: "Serum cystatin C levels to predict serum concentration of digoxin in Japanese patients
... in this study. The serum levels of Cys-C and Cr in the patients were higher than those in the healthy elderly subjects, but the increase of Cys-C was more predominant in the patients. Their ... 1. Introduction Cystatin C (Cys-C) is a non-glycosylated cationic protein of 13.3 kDa, belonging to the cystatin superfamily of cysteine protease inhibitors [1, 2]. Cys-C...
Ngày tải lên: 31/10/2012, 17:08
Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... ⁄ TS50. The analysis of the thermodynamic parameters of the different inhibitors clearly showed an increase of the affinity (decreasing of DG) of the compounds bear- ing the alkyl phosphate chain in ... comparison of binding affinities of the inhibitors under study. The fitting of the binding isotherms of all five com- pounds with a binding model assuming ide...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... by circulating levels of insulin and glucagon [14]. Insulin triggers the activation of a series of phosphorylation cascades that are lost in insulin-resistant states, thereby preventing insulin ... and, in a preliminary investigation using the human hepatoma (HepG2) cell line in this study, we demonstrated the role of TNFa in the regulation of this phenomenon a...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf
... Linear Eyring plots (ln[k/T ]vs.1/T ) were obtained for the hydrolysis of Qf 7 by both enzymes and for the hydrolysis of the substrate containing Val by neurolysin. The Eyring plots for the hydrolysis ... hydrolysis by TOP and neurolysin The preference of cleavage at the L–R or R–R bond for TOP and at the R–R or R–X bond for neurolysin in the case of the su...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo Y học: DAP kinase activity is critical for C2-ceramide-induced apoptosis in PC12 cells ppt
... phosphorylates the regulatory light chain of myosin II the phosphorylation sites being threonine 18 and serine 1 9 [22]. In this study, the synthetic peptide, skeletal myosin light chain kinase substrate ... (pro line 277 to leucine 323) containing the calmodulin-binding domain. In the K42A-DK mutant, a lysine residue at position 42 in t he kinase domain (asterisk) is re...
Ngày tải lên: 24/03/2014, 00:21