Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... compared to oral normal mucosa epithelium. We examined the expression of hMSH2 protein by immunohistochemistry in twenty-six cases of oral lichen planus. Clinically, 12 of them were categorized into ... layers of reticular subtype of oral lichen planus patient (streptavidin-biotin amplified system, x 400). Figure 2. Increased labelling of hMSH2 protein in...
Ngày tải lên: 03/11/2012, 09:54
Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... polypep- tides, all the proteins of interest were present in the soluble fraction of the induced cell in the case of the expression of pET22b(+)/touBEA and /touC, whereas the product of the expression of pET22b(+)/touF ... mass of about 10, 38 and 57 kDa, the expected molecular size of recombinant subunits B, E and A, respectively. The identity of the...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... isopropylthio--D-galactoside induction. This protein was N-terminal His-tagged gAd-GLP-1-A fusion protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion ... quickly separated from the bulk of other bacterial proteins by using metal chelate affinity chromatog-raphy [11]. Because N-terminal His-tag may influence the function of p...
Ngày tải lên: 25/10/2012, 11:10
Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx
... be the start of the protein coding domain. Sequence analysis of the 3¢ end cDNA revealed that there is a polyadenylation signal upstream of the poly(A). Analysis of the primary structure of M. ... same characteristics, the molecular cloning of the corresponding cDNA to get the total amino-acid sequence of the protein, and the expression pattern of the...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... the p76 RBE coding sequence. Position of the introns in the cDNA are indicated by lines and positions in amino acids. Fig. 3. Interaction between p76 RBE and Rho proteins. (A) Using the two-hybrid system, the ... 6). P76 RBE binds the epithelial cytokeratin 18 in vitro With the aim of identifying other proteins interacting with p76 RBE ,p76 RBE -HR1 was used to screen...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
... degree of accuracy, but only if obtained on the same gel. The density of UCP3 protein (i.e. the intensity of the signal expressed per mg of proteins) was similar in the SS mitochondria of the two ... The specific and total levels of COX protein were not significantly different in IMF and SS mitochondria. The yield in mitochondria, which was determined by c...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... histochemical visualization of peroxidase using 3¢,3¢-diaminobenzidine hydrochloride c hromogen (Sigma). The speci®city of the method was tested by the omission of the primary antibody in the staining, and the use of ... development of obesity by both, increasing energy expenditure and d ecreasing in situ lipogenesis. In order to further analyse consequences of r...
Ngày tải lên: 31/03/2014, 15:20
Báo cáo Y học: Expression of recombinant murine pregnancy-associated plasma protein-A (PAPP-A) and a novel variant (PAPP-Ai) with differential proteolytic activity pot
... exceeds other tissues at least 250-fold. Interestingly, the position of the insert of PAPP-Ai, within the proteolytic domain, lies in close proximity to the cysteine residue, which in human PAPP-A ... study of PAPP-A, which must take into account the differences between the mouse and the human. Keywords: metalloproteinase; metzincin; insulin-like growth factors; IGF...
Ngày tải lên: 31/03/2014, 21:21
Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"
... activate the single-strand break DNA repair pathway if applied to host cell DNA. This pathway was investigated by studying the activity of Poly(ADP-ribose)Polymerase (PARP), the protein which ... Upon binding DNA nicks, PARP transfers poly(ADP ribose) (PAR) chains to many of the surrounding proteins, leading to DNA repair and a decrease in the ATP levels of the...
Ngày tải lên: 25/10/2012, 11:18
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... K d of 1.8 m M .These results imply a low affinity site for the magnesium cation in the protein. The incubation of the labeled enzyme with increasing concentrations of adenine nucleotides in the ... structure of E. coli PEP carb- oxykinase (1OEN) is covalently linked to the carbonyl carbon of the P-pyridoxyl group (PL) through an imino linkage. The green line sho...
Ngày tải lên: 21/02/2014, 01:21