Cloning genes by PCR - Tài liệu

Cloning genes by PCR

... Cloning genes by PCR The cloning of genes is often a crucial step in a scientiﬁc project and can be both difﬁcult and time-consuming. The use of PCR has greatly enhanced ... greatly enhanced the successes of gene isolation. Cloning of genes by PCR can be divided into two main areas: (i) genes of known DNA sequence; and (ii) genes of unknown DNA sequence. Genome seque...

Ngày tải lên : 25/10/2013, 22:20

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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

... bacteria by Bourne et al. (2001). The enzyme encoded by the mlrA gene can break open the cyclic structure of microcystin. After opening the ring structure, linear microcystin-LR is degraded by the ... practical biological treatment facility by real-time PCR using the newly designed primer set and a TaqMan probe. The specificity of new primers was investigated using PCR from six mi...

Ngày tải lên : 05/09/2013, 10:15

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Purification and cloning of PCR products

... for cloning PCR products by exploiting the additional A added by Taq DNA polymerase. 116 PCR 6.4 Approaches to cloning PCR products Essentially any cloning vector can be used for cloning a PCR ... blunt- Puriﬁcation and cloning of PCR products 117 Table 6.1 Approaches to cloning PCR products and the vector features necessary for different PCR cloning strategies...

Ngày tải lên : 25/10/2013, 22:20

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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

... Fig. 7. Sequestration of TFIIIB by interactions with the TATA sequences in the ﬂanking regions of tRNA Gly 1 genes. (A) Competition by DNA fragments containing TATATAA sequences. ... rescue of transcription by externally supplemented TFIIIB (lane 5; compare with lane 4) was abolished by the anti-TBP serum (lanes 7, 8). Inhibition was again rescued by increasing concentrations ... M...

Ngày tải lên : 20/02/2014, 02:21

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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... was assessed by calculation of the ratio of radioactivity of the corresponding bands. Quantitative PCR The quantity of mlp genes in mistletoe genomic DNA was assessed by quantitative PCR generally ... gene that encodes an isoform of the protein encoded by ml3p. Cloning of the sequences of mlp genes Approximately 2 kbp products were obtained by PCR with several pairs of spe...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: New human and mouse microRNA genes found by homology search pptx

... from rno-miR-337 by one nucleotide (italic) Differs from rno-miR-345 by two nucleotides (italic) Differs from rno-miR-346 by three nucleotides (italic), and from mmu-miR-346 by two nucleotides hsa-miR-151 ... of 35 new miRNA genes. Similarly, 42 new mouse miRNAs were found by orthology with known human miRNA precursors. Added to those deposited in the miRNA registry, about 200...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: E2A participates in a ﬁne control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot

... mRNA levels of appropriate genes were determined by RT -PCR. The chicken GAPDH gene was used as a control. K. Toyonaga et al. Fine control of pre-mature B-cell apoptosis by E2A FEBS Journal 276 (2009) ... determined by semi-quantitative RT -PCR using appropriate primers. The chicken GAPDH gene was used as an internal control. The numbers under the panels indicate the number of cycles...

Ngày tải lên : 16/03/2014, 04:20

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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf

... produc- tion in strain FI9078 was mediated by the enzyme encoded by the ldhB gene. As expected, the enzyme pro- duced by strain MG1363 was encoded by the ldh gene. LDHB (ldhB gene product) and ... replacing LDH by LDHB on the physiology of L. lactis was assessed by monitoring the evolution of the pools of glycolytic intermediates and cofactors during the metabolism of glucose by...

Ngày tải lên : 16/03/2014, 05:20

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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

... in several tissues by RT -PCR but not by tissue section in situ hybridization. The differential and complement- ary expression patterns of the zebraﬁsh crabp1a and crabp1b genes imply that subfunctionalization ... continuous neurogenesis [30–32]. This generation of new neurons in the adult CNS most assuredly requires the expression a speciﬁc subset of neuronal genes. One of the gen...

Ngày tải lên : 16/03/2014, 22:20

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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot

... describe the pathogenesis and genetic predisposition of GD and HT first, followed by illustrating those of GO. Finally, we discuss the upcoming problems in future research. 2. The pathogenesis of Graves’ ... decades, identifying numerous genes, of which some have proven to be significant genetic factors in GD pathogenesis. In the next section, we describe these susceptibility loci. Genes...

Ngày tải lên : 17/03/2014, 21:20

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