Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid

Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"

... in the reduction of B19 NS1- induced cytotoxicity of liver cells. Our results support the hypothesis that in the setting of persistent B19 infection, direct B19 NS1- induced cytotoxicity may play ... B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture Violetta Kivovich 1,2 , Leona Gilbert 2, Matti...

Ngày tải lên : 26/10/2012, 09:32

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Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"

... induc-tion of protein expression. Caffeine was maintained on the cells during expression of GFP or GFP /NS1. PARP was inhibited by incubating transfected cells with 5-aminoisoquinolinone (Calbiochem) ... natural B19, while a mutant of NS1 with the NTP binding region deleted induced significantly less apoptosis (20). The GFP /NS1 vector was utilized in this...

Ngày tải lên : 25/10/2012, 11:18

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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx

... protein of 318 amino acid residues. The recombinant enzyme was functional and readily detected by assay of TSO hydrolysis in yeast extracts upon over-expression in P. pastoris using a methanol inducible ... and 249, respectively. Neither of the two variants resulted in amino acid substitutions, however. The predicted translated product of the cDNA contained 318 amino a...

Ngày tải lên : 31/03/2014, 08:20

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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

... short peptide including 15 amino acids: [N-(Serine-glycine)7- Serine-C], which was used as a linker to connect the N-terminus of gAd and the C-terminus of GLP-1-A. Because the protein produced ... Circulating GLP-1 is inactivated after cleaving the first two amino acids at the N-terminus by dipeptidyl peptidase IV [14]. Studies reported that the replacement of alanine with gly...

Ngày tải lên : 25/10/2012, 11:10

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Báo cáo y học: "Effect of Acute Administration of an Herbal Preparation on Blood Pressure and Heart Rate in Humans"

... p-synephrine [8-11]. The safety of p-synephrine is clouded by its structural similarity to nor-epinephrine in spite of the fact that the pharma-cokinetics of the two compounds and the ... properties of p-synephrine (hydroxyl group on the para position of the benzene ring) which is found in C. aurantium, and m-synephrine (phe-nylephrine; with the hydroxyl group on the met...

Ngày tải lên : 25/10/2012, 11:10

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Báo cáo y học: " Post-traumatic glioma: Report of one case and review of the literature"

... should be in direct continuity with the traumatic scar, not merely in its vicinity or separated by a narrow zone of healthy or slightly al-tered brain tissue. The stringent criteria for diagnosing ... a cocarcino-gen in the presence of an initiating carcinogen 2. It was hypothesized that cells damaged by the initiating carcinogen proliferated as a natural result of the trauma,...

Ngày tải lên : 25/10/2012, 11:48

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Báo cáo y học: "TPO, but not soluble-IL-6 receptor, levels increase after anagrelide treatment of thrombocythemia in chronic myeloproliferative disorders"

... receptors Introduction One of the clinical challenges in chronic mye-loproliferative disorders (MPD) is thrombocythemia, always present in essential thrombocythemia (ET) and often in polycythemia ... thereby reducing megakaryocyte size and ploidy, and disrupting or preventing full megakaryocyte matura-tion. However, little is known of the interaction of anagrelide with cytokines reg...

Ngày tải lên : 03/11/2012, 10:52

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Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt

... crosslinking studies indicated the localization of the coupling ion binding site in the Na + -translocating F 1 F 0 ATP synthase of Ilyobacter tartaricus within the hydrophobic part of the bilayer. ... various times and diluted into 1 mL of the assay mixture. Determination of ATP hydrolyzing activity The coupled enzyme assay was used to determine ATP hydrolyzing activity of t...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf

... lipoxygenase by binding to the reduced form (Fe 2+ )oftheenzyme thereby keeping it inactive [49]. In our experiments NDGA was effective in inhibiting the activity of puriﬁed recombinant MTE-I in an in ... is unlikely. This, along with the fact that 22R-OH-cholesterol by-passes the effect of the inhibitors strongly indicates that the thioesterase and the acyl CoA-synthe...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

... Furthermore, binding of NO trans to the histidine has been found to result in cleavage of the HisN–Fe bond in guanylate cyclase [22]. In the case of guanylate cyclase, the cleavage of the HisN–Fe ... difference in enzymatic activity. As expected for heme enzymes, the ferric heme in the enzyme is primarily ﬁve-co-ordinate at the pH of optimal activity. Thus the ferr...

Ngày tải lên : 08/03/2014, 16:20

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