... 1 h at 42 °C.The cDNA was amplified using the rat HSP60 senseprimer (5¢-CAAATGAAGAGGCTGGGGATGGCA-3¢) and antisense primer (5¢-GAGCAGGTACAATGGACTGAACAC-3¢) in a 50-lL reaction volume containing200 ... thepresence of an equal molar ratio of HSP60 (n), an equal molar ratio of HSP60/HSP10 (e), an equal molar ratio of HSP60 and 5 mMATP/Mg (m), and an equal molar ratio of HSP60/HSP10 and 5 mMATP/Mg ... H., Komatsuda, A. , Ohtani, H.,Miura, A. B., Otaka, M., Masamune, O., Andoh, H., Koyama, K.,Sato, Y. & Tashima. Y. (1995) Mammalian 60-kDa stress protein(chaperonin homolog): Identification,...