Báo cáo y học: " Experimental ablation of the pancreas with high intensity focused ultrasound (HIFU) in a porcine model"
Ngày tải lên: 25/10/2012, 11:18
Ngày tải lên: 25/10/2012, 11:15
... N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic functions in ... lipase activity and a normal esterase activity, displays a substantial conformational change in the substrate-binding site in the C-terminal domain (red arrow). Mode...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... not change the catalytic activity of D-chymotrypsin. Molecular stability was determined by measuring heat inactivation, which was found to be a sensitive marker of the stability of chymotrypsin [13,22– ... D-chymotrypsin to tryptic cleavage was the same as seen from their inactivation rates in the presence of equimolar amounts of trypsin (Table 2). The cleavage of t...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc
... exponential of the matrix of theoretical cross relaxation rates. These values may be replaced by scaled experimental values and the logarithm of the matrix may be taken to obtain distances that take account ... molecule and because of inaccuracies in the starting structure. Although mathemat- ical convergence may be achieved by iterative calculations, the accuracy of...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
... interpretation, the relative intensities of thetwoformsintheX-raystructureandtheEPRspectra agree, we are aware that a number of factors may in uence these ratios: differences in pH and physical state ... addition of a flavone (apigenin), of a flavanonol (taxifolin) and of a flavan-3-ol (epicatechin) (Fig. 8) did not alter the EPR spectra of the enzyme indicating the...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... units; pNP, 4-nitrophenyl group; PPA, porcine pancreatic a- amylase. Enzymes: a- amylase (EC 3.2.1.1); porcine pancreatic a- amylase (AMYP_PIG); barley a- amylase (AMY2_HORVU); rice a- amylase. (Received ... barley a- amylase isoenzyme. The binding a nities were calculated according to the data of Table 2. Fig. 6. Subsite map of rice a- amylase isoenzyme ( AMY 3 D ). The...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... produced the protein in mammalian cells. In order to facilitate the proteins’ purification, a recombinant version was made containing a C-terminal myc tag followed by a His 6 sequence, by cloning the ... unidentifiable by autoradiography in cell lysates, but appeared as the predominant band after purification, although a background of contamination by other proteins was s...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF system mass spectro- meter and a- cyano-4-hydroxycinnamic acid as matrix. Sample preparation It has been shown that a ... ellipticity increases with the water concentration, reaching a plateau at approximately 20% water. Corres- pondingly, the helix content, as estimated by standard linear combinati...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... 9S regulatory particles (RP; 700 kDa). The 20S proteasome has a cylindrical structure, consisting of a and b rings stacked in the order of abba, and each ring contains seven structurally related a and ... Biological Research/Graduate School of Integrated Science, Japan, personal communication) iden- tified N-acetylation of many of the yeast 26S proteasome subunits (a1 ,...
Ngày tải lên: 08/03/2014, 16:20