Báo cáo y học: " Experimental ablation of the pancreas with high intensity focused ultrasound (HIFU) in a porcine model"
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding and catalytic functions in ... lipase activity and a normalesterase activity, displays a substantial conformationalchange in the substrate-binding site in the C-terminaldomain (red arrow). Modeling of the K41 4A mutant,which ... domain (40% similarity) and are28% identical in the C-terminal domain (38% similarity). The catalytic efficiency and heparin-binding functions of the N-terminal domain have been extensively studied[13,14]....
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... not change the catalytic activity of D-chymotrypsin. Molecular stability was determined bymeasuring heat inactivation, which was found to be a sensitive marker of the stability of chymotrypsin[13,22– ... D-chymotrypsin to tryptic cleavage was the same as seenfrom their inactivation rates in the presence of equimolaramounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loopsTo ... in the interdomain loop (at Phe114). The lack of temporary accumulation of fragment III during the degradation of the Phe114 !Ile interdomain loop mutantclearly indicates that the cleavage of...
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Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc
... exponential of the matrix of theoretical cross relaxation rates. These valuesmay be replaced by scaled experimental values and the logarithm of the matrix may be taken to obtain distances thattake account ... molecule and because of inaccuracies in the starting structure. Although mathemat-ical convergence may be achieved by iterative calculations, the accuracy of the distances obtained remains uncertain.Therefore, ... 2001geometry, the set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration...
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Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
... interpretation, the relative intensities of thetwoformsintheX-raystructureandtheEPRspectraagree, we are aware that a number of factors may in uencethese ratios: differences in pH and physical state ... addition of a flavone (apigenin), of a flavanonol (taxifolin) and of a flavan-3-ol (epicatechin)(Fig. 8) did not alter the EPR spectra of the enzymeindicating the absence of binding to the copper ... example, the gzzand gyyvalues are much closer to each other,indicating a substantial perturbation of the axial symmetry of the tensor, whereas axial symmetry, i.e. gxx% gyy<< gzzis typically...
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Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... units;pNP, 4-nitrophenyl group; PPA, porcine pancreatic a- amylase.Enzymes: a- amylase (EC 3.2.1.1); porcine pancreatic a- amylase(AMYP_PIG); barley a- amylase (AMY2_HORVU); rice a- amylase.(Received ... barley a- amylase isoenzyme. The binding a nities were calculated according to the data of Table 2.Fig. 6. Subsite map of rice a- amylase isoenzyme (AMY3D). The binding a nities were calculated ... is aneffective way to explore the nature of the binding site and the process of catalysis for a- amylases. Although the overallstructure and the tertiary folding of the polypeptide chainsof...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... produced the protein in mammalian cells. In orderto facilitate the proteins’ purification, a recombinant versionwas made containing a C-terminal myc tag followed by a His6sequence, by cloning the ... unidentifiable by autoradiography in celllysates, but appeared as the predominant band afterpurification, although a background of contamination byother proteins was still present. The second ... present on the plasmamembrane was quantified by FACS analysis using the 2D7antibody (Fig. 2B). It was found that the 60–70% of the myc-his tagged protein was internalized, as much as forwild-type...
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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF system mass spectro-meter and a- cyano-4-hydroxycinnamic acid as matrix.Sample preparationIt has been shown that a ... ellipticity increases with the water concentration,reaching a plateau at approximately 20% water. Corres-pondingly, the helix content, as estimated by standard linearcombination fits of the spectra ... stronglyhydrophobic side chains; this feature has been aptlydescribed by Rajan et al. as a Teflon coating that cansurround a helix [16] in the case of a mixture of water andhexafluoroacetone hydrate,...
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... 9Sregulatory particles (RP; 700 kDa). The 20S proteasomehas a cylindrical structure, consisting of a and b ringsstacked in the order of abba, and each ring contains sevenstructurally related a and ... Biological Research/Graduate School of Integrated Science, Japan, personal communication) iden-tified N-acetylation of many of the yeast 26S proteasomesubunits (a1 , a2 , a3 , a4 , a5 , a6 , a7 , a3 , a5 , ... 1475Protein assay The protein concentration of the sample was d etermined by the Bradford method [17] using c-globulin as a standard.Western blotting analysis The subunits of rice RP were separated...
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