Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

... con-centration. In vitro releasing study The release of pravastatin as well as hemoglobin from carrier erythrocytes were determined as follow-ing, 1 ml of packed drug-loaded erythrocytes was diluted ... temperature as well as concentration on the loading of pravastatin in human erythrocytes to be using them as systemic sustained release delivery system for...

Ngày tải lên: 25/10/2012, 11:10

... responsible for the four electron reduction of dioxygen [3]. These enzymes are common in plants, fungi, insects and bacteria [1]. In plants, they may mainly play a role in lignification [4] whereas in fungi ... differences in kinetics observed may be the consequence of the variability of certain of the amino acids involved in the substrates channel in specific enzymes. A...

Ngày tải lên: 08/03/2014, 09:20

... recombinant human insulin and human transferrin from Wako, Osaka, Japan; and nickel- nitrilotriacetic acid agarose from Qiagen Inc., Chatsworth, CA, USA. Recombinant human thioredoxin was kindly Correspondence ... requiring iron [41]. Albumin is believed to supply many low-molecular-mass substances such as metals, amino acids and fatty acids [42]. Transferrin and albumin noncovalently...

Ngày tải lên: 31/03/2014, 08:20

... conserved as insect ACE can hydrolyse mammalian ACE substrates such as angiotensin I, substance P, lutein- izing hormone releasing hormone, enkephalins and enkeph- alinamides, hereby displaying the ... as present in the N-terminal part of several y yolk proteins. An antiserum raised against Neb-ODAIF-1 1)9 immunostained one out of three yolk protein bands of SDS/PAGE-separ- a...

Ngày tải lên: 24/03/2014, 03:21

... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... University Institute of Life Sciences (Hangzhou, China). Mouse anti-His-tag monoclonal antibody was purchased from Novagen Company (Germany). Streptozocin was obtained from Sigma Compa...

Ngày tải lên: 25/10/2012, 11:10

... Hepatitis. Clinical epidemiology of ful-minant hepatitis in Japan before the substantial introduction of liver transplantation: an analysis of 1309 cases in a 15-year na-tional survey. Hepatol ... These findings indicate the difficulty in amplifying the sequences in these re-gions. In our study, we attempted to amplify each ex-tremity, but failed. Interestingly, phylogenetic...

Ngày tải lên: 26/10/2012, 10:03

... proliferation of high-density EEG methods, and interdisciplinary interest in its application as a prognostic, diagnostic, and investigative tool, an understanding of the underpinnings of P300 and N200 physiology ... utilizing the auditory oddball paradigm. In one such study examining frequency and intensity deviation, changes in inter-stimulus interval (ISI) and intensity in...

Ngày tải lên: 02/11/2012, 11:08

... no information is available on the regulation of DNase I gene (DNASE1) expression in vivo or in vitro. We first mapped the transcription start sites of DNASE1 in human pancreas and in the DNase ... or hypoxic conditions prior to the measurement of the activit- ies of fire y and Renilla luciferase. Cell lysis and luciferase assays were performed using the Dual Luciferase Repo...

Ngày tải lên: 19/02/2014, 06:20

... milliliter of S-hexyl glutathione agarose. In all cases, protein purity was determined by SDS/PAGE and protein concentration was measured by bicinchoninic acid method following manu- facturers indications ... 8.0, and eluted by adding 10 lLofthe same buffer containing 5 m M GSH, GSSG or S-hexyl GSH. Ten microliters of the original protein, binding assay supernatant and eluate were analy...

Ngày tải lên: 21/02/2014, 01:21

... behaviour, indicating that the same disulfide(s) are involved in maintaining the integrity of active protein structure in both wild-type and recombinant VPR. The wild-type and recombinant forms of the ... in psychrophilic enzymes. Glycine residues are highly con- served in the sequences of the proteinases compared in this study. For example, of the 37 glycines present in...

Ngày tải lên: 21/02/2014, 01:21