Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo Tongfeng Zhao1, Jing...
Ngày tải lên : 25/10/2012, 11:10
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

... Serotonin (5-hydroxytryptamine) interacts with N-acetylneuraminic acid, which is often contained in glycolipids and glycoproteins on the cell surface [24]. We examined the synergistic effects of ... (1986) Involvement of finger domain and kringle 2 domain of tissue-type plas- minogen activator in fibrin binding and stimulation of activity by fibrin. EMBO J 5, 3525–3530. 37 Bakk...
Ngày tải lên : 17/03/2014, 17:20
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 Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"

Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"

... layers of reticular subtype of oral lichen planus patient (streptavidin-biotin amplified system, x 400). Figure 2. Increased labelling of hMSH2 protein in basal and intermediate epithelial layers ... counterstained with Meyer’s haematoxylin and mounted in PermountTM. Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in...
Ngày tải lên : 03/11/2012, 09:54
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... classes. Role of Tyr183 in catalysis In previous crystallographic studies it was shown that the hydroxyl side chain of Tyr183 is within hydrogen bonding distance of the N-acetyl carbonyl oxygen of the ... activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify the recombi...
Ngày tải lên : 22/02/2014, 04:20
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf

Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf

... the combination of the isoforms is yet to be determined. We are now trying to express fusion proteins of A. acetabulum proteolipid subunits and green fluorescent protein in A. acetabulum and tobacco ... complementability of the group 2 proteins for the yeast vma3-mutant. A comparison of the structures, especially in the four hydrophobic domains (underlined sequences in Fi...
Ngày tải lên : 08/03/2014, 23:20
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx

Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx

... PRISM (http://www.graphpad.com). Single turnover assay Single turnover assays of the individual components (10nmolofTomoH,20nmolofTomoCandTomoD subunits) and of each of their possible combinations, were performed by adding ... mass of about 10, 38 and 57 kDa, the expected molecular size of recombinant subunits B, E and A, respectively. The identity of the proteins was furt...
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... acid synthesis. Indeed, synthesis of blood coagulation proteins in vertebrates and toxins in the cone snail may be secondary functions of this enzyme. ACKNOWLEDGEMENTS We especially appreciate ... (1997) The active site region of the vitaminK-dependent carboxylase includes both the amino-terminal hydrophobic and carboxy-terminal hydrophilic domains of the protein. FEBS Lett....
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx

Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx

... after 40 cycles consisting of 1 min at 94 °C, 1 min at 44 °Cand1minat 72 °C in a Hybaid thermocycler. Subcloning in pZERO (Invitrogen) using EcoRI and BamHI resulted in loss of a part of the amplified ... guanine nucleotide-binding proteins: the G proteins. G proteins play a central role in a wide variety of signal transduction pathways, mediating the perception of environm...
Ngày tải lên : 17/03/2014, 23:20
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx

Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx

... the site of injection Injection of microorganisms resulted in a dramatic decrease in numbers of both circulating and tissue in ltrating haemocytes within 3 h of injection. To study haemocyte behaviour ... immunoreactivity observed at the site of microbial injection precluded both clarification of the mechanisms of penaeidin release from haemocytes, and determination of...
Ngày tải lên : 18/03/2014, 01:20
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx

... Kuhne, Pierre Flandin, Jean-Paul Giacobino and Patrick Muzzin Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland Uncoupling protein- 3 (UCP3) is a mitochondrial inner- membrane ... specific and total levels of COX protein were not significantly different in IMF and SS mitochondria. The yield in mitochondria, which was determined by co...
Ngày tải lên : 24/03/2014, 04:21
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