Báo cáo y học: "Randomized trial comparing daily interruption of sedation and

Báo cáo y học: "Randomized trial comparing daily interruption of sedation and nursing-implemented sedation algorithm in medical intensive care unit patients"

9
605
0

Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc

... analysis of the binding of E. coliRuvA to synthetic Holliday junctions using a variety of biochemical procedures. Speciﬁcally we investigatedprotein–DNA interactions involving the RuvA and RuvC ... showing the DNA binding speciﬁcity of RuvA.(A) Binding of wild-type RuvA (2 lM) and (B) binding of the mutantRuvA (E55R D56K) (1.2 lM) to Holliday junction, linear duplex and 3-strand junction ... binding and instrument drift.RESULTSStoichiometry and kinetics of the RuvA–Hollidayjunction interactionThe stoichiometry and kinetics of the RuvA–Hollidayjunction interaction was analysed...

10
672
0

Báo cáo Y học: Artocarpus hirsuta lectin Differential modes of chemical and thermal denaturation potx

... 2MGdnHCl), showing increase in t he ﬂuorescence intensity,indicating temporary exposure of the hydrophobic patches of the protein during unfolding (Fig. 3B). The p ossibility of the occurrence of the ... lM)andANS(50lM), in different concentra-tions of GdnHCl (1–5M).Determination of the lectin activitySugar binding activity of the samples was measured by theenhancement in the intrinsic ﬂu orescence of ... highspeciﬁcity f or methyl a-D-galactopyranoside (Me a-gal).The folding pathways of oligomeric proteins involve bothintramolecular and intermolecular interactions. The dena-turation of pea and peanut...

5
374
0

Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot

... thatpoly(b-L-malate) is active in maintaining mitotic synchrony of plasmodia b y playing the mediator between the periodicsynthesis of certain proteins and the catalytic competence of DNA po lymerases.Keywords: ... forms in the life c ycle [4]. Interestingly, thebillions of nuclei in these syncytia participate with highdegree of synchrony in the division cycle. All of themyxomycetes species so far examined ... mMspermineÆ4HCl or2mMspermidineÆ3HCl were included to suppress binding and inhibition of polymerases by PMLA [1]. One unit of polymerase activity is equivalent to the amount of enzymethat catalyses...