Báo cáo y học: "Users' guides to the medical literature: how to use an article about mortality in a humanitarian emergency"

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

. As both the E a of inactivation and the temperature at which the enzyme starts inactivating at a measurable rate increase from the apoenzyme to the Mn 2þ -containing enzyme, loss of the metal cofactor. corresponding dialysis buffer. The dialysis buffer was used to generate the baseline. The enzyme containing both Mg 2þ and Co 2þ was dialyzed against buffer A, then scanned against the dialysi...

Ngày tải lên: 22/02/2014, 07:20

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Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot

Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot

. doi:10.1046/j.1432-1033.2002.02997.x designed to maintain hydrophobicity and Gly, Ser, Asp and His mutants to provide hydrophilicity. The catalytic roles of Cys341 in carboxypeptidase Y are examined by comparing the kinetic parameters. also suggest that Cys341 may be involved in the catalysis in a manner other than substrate binding. However, the precise type of catalytic event affected by Cys...

Ngày tải lên: 08/03/2014, 23:20

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