... shown).Galectin 3 and CD98 co-immunoprecipitateWe have previously postulated a role for galectin 3,an S-type lectin containing a carbohydrate-bindingdomain, as a physiological ligand of CD98 in vivo ... with1% saponin in FACS buffer for 15 min at room tempera-ture, and then stained following the surface staining proto-col. After the final wash, samples were fixed again in 2%PFA before analysis.ImmunofluorescenceCells ... F, Artigues A, Castagna L & Osdoby P(2002) New alternatively spliced form of galectin 3, a member of the beta-galactoside-binding animal lectinfamily, contains a predicted transmembrane-spanningdomain...