... T1Cu and T2Cu is thefastest of the three redox processes involved in the catalysis:(a) reduction of T1Cu; (b) oxidation of T1Cu by T2Cu; and(c) reoxidation of T2Cu by NO2–. Electrochemical ... After reduction of the T2Cu center with anelectron from the T1Cu center, the proton of this hydrogenbond is transferred from the Asp98 residue to the oxygenatom of the substrate, yielding an ... accept electrons from theazurin isolated from the same strain at moderate rates.Spectroscopic characterization allowed, for the first time,the determination of the redox potentials of both coppercenters....