Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên: 05/09/2013, 09:08

... immobilized mitochondrial import receptor Tom70 Tom70 is an integral membrane protein consisting of a large globular receptor domain exposed to the cytosol and a short N-terminal transmembrane anchor. ... transformed into E. coli TG1 and phagemid particles rescued and panned against the immo- bilized Tom70 antigen. Four rounds of biopanning were performed with an increase in t...

Ngày tải lên: 17/03/2014, 10:20

... P III and P IV) were isolated by affinity, ion exchange and chromatofocusing chromatographies, and investigated with respect to toxicity and sugar binding specificity. Half maximal inhibitory concentration ... inhibitory concentration and median lethal dose values indicate that P I and P II have similar toxicities and that both are more toxic to cultured HeLa cells and mice...

Ngày tải lên: 18/02/2014, 16:20

... curves. Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5. Triangles and continuous line: data and fitting with PHI/PHO ratio of 1. Asterisks and broken line: data and fitting with ... (University of Turin) for mass spectroscopy analysis, to A. Conti and G. Giuffrida (CNR- Torino) for N-terminal sequence determination and to D. Cavazzini (University of...

Ngày tải lên: 21/02/2014, 00:20

... profile Figure 1A shows the SDS/PAGE of purified ccNiR upon different treatments. The complex dissociates into an intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming ... nature (Fig. 1, lane 1). However, in the absence of boiling (Fig. 1A, lanes 2 and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as we...

Ngày tải lên: 21/02/2014, 00:20

... & Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®c antioxidant from m ammalian brain: alkyl reductases and thiol- speci®c antioxidant de®ne a large family o f antioxidant enzymes. Proc. N atl ... east and Arabi dopsis, and has been shown to be thioredoxin-dependent and function in a similar manne r to 2 Cys-Prx [ 8,9]. 2Cys-Prx catalyzes, in vitro, the red uction...

Ngày tải lên: 08/03/2014, 16:20

... according to [56] using BSA as a protein standard. The DNA and amino acid sequence analyses and prediction of the molecular masses were performed with the programs mapdraw and protean in dnastar ... images of the trans- formed protoplasts are shown in (A; pGFP-N fusion) and C; pGFP-C fusion). Bright field images of the same protoplasts were made to visualize the protoplast’s...

Ngày tải lên: 16/03/2014, 18:20

... rotor (Eppendorf, Hamburg, Germany). The supernatant was collected and split into two equal samples: to one was added 2 lLH 2 O and to the other was added 2 lL Endo H (0.005 U lL )1 ) (F. Hoffmann-La ... random priming and the incorporation of [a- 32 P]dCTP. Hybridization of the probe to the membrane and subse- quent washes were performed as described [29]. Hybridiza- tion w...

Ngày tải lên: 23/03/2014, 07:20

... struc- ture of human and bovine MUC15, and exon skipping as a possible explanation for the origin of the splice variant. Interestingly, the appearance of alternative soluble variants of membrane-associated ... node, tonsil, and fetal liver. Furthermore, PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland,...

Ngày tải lên: 24/03/2014, 04:21

... of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails. A ... with [ 32 P]ATP[cP] and T4 polynucleotide kinase. The labeled oligomers were annealed by heating to 90 °C an equimolar mixture of the upper and lower strands in r...

Ngày tải lên: 30/03/2014, 13:20