Reproduction Methods

... individuals are produced through sexual reproduction 6/7 Reproduction Methods parthenogenesis budding fragmentation A Free Response Why is sexual reproduction useful if only half the animals ... males Sexual Reproduction Sexual reproduction is the combination of (usually haploid) reproductive cells from two individuals to form a third (usually diploid) unique offspring Sexual rep...

Ngày tải lên: 31/10/2017, 00:45

... protective clothing and equipment Back Back to Hazards, Health and Safety homepage Back to Hazards, Health and Safety homepage Be sun safe Safety tips for working outdoors on hot, sunny days A good proportion ... Hazards, Health and Safety homepage Back to Hazards, Health and Safety homepage Workplace health and safety explained What does it all mean? In simple terms, workplace health and s...

Ngày tải lên: 21/08/2012, 17:56

Ngày tải lên: 17/09/2012, 09:13

... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; ... correct volume of 10 Davies and Carlstedt ice-cold dry propan-1-ol to give a 100 mM solution DFP is unstable in water but can be stored at –20°C in propan-1-ol After dilution, the vial as w...

Ngày tải lên: 23/09/2012, 19:25

... to T-antigen (β-d-Gal 1-3 GalNAc), but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 / 4GlcNAc) (19) Demonstration of PNA binding is not necessarily evidence of T-antigen ... Blue 8GX in 0.1 N HCl (pH 1.0) HID: Dissolve 120 mg of N,N-dimethyl-m-phenylenediamine dihydrochloride (Sigma) and 20 mg of N,N-dimethyl-p-phenylenediamine dihydrochloride (Sigma) in 5...

Ngày tải lên: 23/09/2012, 19:25

... nonionic detergents: 0.05% (v/v) Nonidet P-40 or Tween-20 Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL, A405nm), O-phenyldiamine (OPD) (1 mg/mL, A492nm), ... chromogens that leave a colored precipitate on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix for HRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazoli...

Ngày tải lên: 23/09/2012, 19:25

... 250 mg of phenol in 70 mL of glycerol Poly-L-lysine-coated slides: slides are immersed in 0.01% (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of ... A., Allen, A., Morris, E., and Ross-Murphy S B (1988) Mucus glycoprotein gels: role of glycoprotein polymeric structure and carbohydrate side-chains in gel-formation Carbohydrate Res 178, 93–1...

Ngày tải lên: 23/09/2012, 19:25

... as [35S]sulfate-labeled, TCA-precipitable proteins (cpm/mL) (see Note 10) g Mature [35S]sulfate-labeled, mucin band on 4% SDS-PAGE (au/mL) h Total concentration of mucin by dot-blotting (au/mL) ... before use, from 100 mM stock solution in 2-propanol 21 Blotto (wash buffer for Western-type dot-blots): 50 mM Tris (pH 7.8), mM CaCl2, 0.05% (v/v) Nonidet P-40 (BDH), 0.01% antifoam A (Sigma), 5%...

Ngày tải lên: 23/09/2012, 19:25

... advisable We analyze aliquots of the peaks by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and purify peptides to homogeneity by rechromatography ... effectiveness of the separation is monitored with a periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the rela...

Ngày tải lên: 23/09/2012, 19:26

... alternative, however, is rate-zonal centrifugation, which separates molecules not on hydrodynamic volume alone but on their mass-to-volume ratio as well The basis of rate-zonal centrifugation for ... Distribution of Gel-Forming Mucins 89 Fig Schematic diagram of gradient-forming apparatus We next describe an experiment that involves the simultaneous preparation of × 12 mL (in 13-mL tubes) 6–8...

Ngày tải lên: 23/09/2012, 19:26

... of mucin glycoproteins The hydrolysate of mucin is then derivatized with Fmoc on the α-amino groups under alkaline conditions The derivatized amino acids are separated by a C18 reversed-phase column ... continuous flow of helium Hypersil C18 reversed-phase column: 150 × 4.6 mm inner diameter µm (Keystone, Bellefonte, PA) In-line filter (2 µm) (Upchurch, cat no 10 0-1 0) 2.2 Chemicals 2.2.1...

Ngày tải lên: 23/09/2012, 19:26

... degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation Biochem Biophys Res Commun 178, 1194–1201 Laursen, R A (1971) Solid-phase Edman degradation: an automated ... (C) The glycosylated amino acid window with elution profiles of PTH-threonine-GalNAc-Gal (black arrowheads) and PTH-threonine-GalNAc (gray arrowheads) from the Applied Biosystems Procise Standard ......

Ngày tải lên: 23/09/2012, 19:26

... protecting group t-Butyl ether t-Butyl ester t-Butoxycarbonyl 2,2,5,7,8-Pentamethylchroman-6-sulfonyl Trityl Amino acid S, T, Y D, E K, H, W R C Materials 2.1 Preparation of BSA-Peptide Conjugates ... 16.8 g of citric acid monohydrate made up to L with distilled water, pH 4.0 2,2'-azino-bis[3-ethylbenz-thiazoline-6-sulfonic acid] (ABTS) substrate solution (Sigma): 0.5 mg/mL in citrate phospha...

Ngày tải lên: 23/09/2012, 19:26

... sterilized 20 mM benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (benzyl-α-GalNAc) (Sigma), filter sterilized Peptide-N 4-( acetyl-ß-glycosaminyl) asparagine amidase (N-glycosidase F, EC 3.5.1.52) ... chloride and 1.65 mg of 5-bromo-4chloro-3-indolyl-phosphate), and stop solution (10 mM Tris-HCl, mM EDTA, pH 8.0) Nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP)...

Ngày tải lên: 23/09/2012, 19:26

... monosaccharides by reversed-phase HPLC Ultrasphere ODS (4.6 × 250 mm) 1, PA-Gal; 2, PA-Glc; 3, PA-Man; 4, PA-Rib; 5, PAFuc; 6, PA-Rham; 7, PA-ManNAc; 8, PA-deoxy-Rib; 9, PA-GlcNAz; 10, PA-GalNAc; 11, PANeuAc ... Oligosaccharide-alditols released by Type of reagents Detection Sensitivity HPLC Benzoyl p-Bromo-benzoyl; naphtoyl-benzoyl Dns-hydrazide DABS hydrazide Fmoc hydrazide Dansyl-hydrazide...

Ngày tải lên: 23/09/2012, 19:26