Mode sensors and switches - P5

Glycoprotein Methods and Protocols - P5

Glycoprotein Methods and Protocols - P5

... homogenate and medium byWestern-type dot-blot procedure. Spot aliquots of the homogenates or media on nitrocel-lulose paper, using the dot-blot apparatus and air-dry for 5 min. Perform all ensuing pro-cedures ... [35S]sulfate-labeled, TCA-precipitable proteins in tis-sue homogenate (j1) and medium (j2) (cpm/mL) (Note 10).k. Mature [35S]sulfate-labeled mucin band in tissue homogenate (k1...

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