Effect of Secondary Structure on Biological Activities of Antimicrobial Peptides

... order to demonstrate the effect of the secondary structure of the helical amphipathic peptides on their cytotoxic activity and antimicrobial specificity, peptides with different secondary structures ... the importance of a lack of structure in nondenaturing conditions but an inducible structure in the presence of the hydrophobic environment of the membrane, (ii...

Ngày tải lên: 24/10/2016, 11:20

... 23 2. 2.1 Extraction of diclofenac (3) from Voltaren tablets 24 2. 2 .2 Synthesis of compounds 1, and 25 2. 2 .2. 1 Synthesis of 2- iodophenyl-N, N-dimethylacetamide (25 ) 25 2. 2 .2. 2 General ... Statement of purpose 19 Chapter Design and Synthesis of 2- Phenylaminophenylacetic Acid Derived Compounds 22 2. 1 Introduction 22 2. 2 Experimental methods 23...

Ngày tải lên: 09/09/2015, 11:27

... divorced from consideration of the effect of the therapy received In addition to assessing delay in institution of therapy, analysis of the effect of appropriateness of therapy must take into account ... but clinically problematic, the effect on mortality of delay in receiving truly appropriate therapy, and the potential impact of this delay on the results of the...

Ngày tải lên: 13/08/2014, 11:23

... bilayer 14 1 CHAPTER CONCLUSION AND OUTLOOK 8 .1 Conclusion 8.2 Outlook 18 4 18 4 18 9 Bibliography 19 2 14 1 14 1 14 5 14 5 14 5 14 6 14 8 15 1 15 3 15 5 15 5 15 8 16 3 16 8 17 0 17 0 17 1 17 6 18 2 iv Summary Antimicrobial ... antimicrobial peptides 75 78 78 79 84 84 84 87 89 90 90 92 94 95 95 97 99 10 0 10 1 10 1 10 2 10 5 10 5 11 3 11 9 12 3 12 8...

Ngày tải lên: 14/09/2015, 21:54

... 7 .3 Results and discussion 7 .3. 1 Isotherm studies of V4 interacting with POPG and POPC 7 .3. 1.1 Isotherms of lipid monolayers The surface pressure (π) and molecular area (A) isotherms for POPG and ... difference between the POPG and POPC isotherms, indicative of the negligible effect of electrostatic interaction 145 Fig 7.1 Isotherms of POPG and POPC mon...

Ngày tải lên: 14/09/2015, 23:32

... fitting The second particle exhibited an F2 of 4 .2 % and an increase in the fluorescence yield compared to TMR of Q2/Q1 of 3 .21 90 Fig 5.5 Comparison of ACFs of V4-TMR and the complexes of V4-TMR with ... 0.6 and 1.7 ms, similar to the expected diffusion time of lipid SUVs However, F2 and N2 differed markedly depending on the lipid used In the group...

Ngày tải lên: 14/09/2015, 23:32

... addition of Co2+ ion content, which may be attributed to the ionic radii of constituent ions causing increase in lattice parameter and the densities of pure cobalt ferrite (5.29 g/cm3) and pure nickel ... temperature.These plots show that an increase in Co2+ doping yields monotonic increase in the saturation magnetization of Niferrite which may be due to the substit...

Ngày tải lên: 13/02/2014, 04:20

... the anion protein interactions in A-state stabilization Competition among anions To better define the effect produced by monovalent anions on the sulfate-induced A-state of cytochrome c, we monitored ... environment of the sulfate-induced A-state of cytochrome c, as observed from changes induced in the 416 nm Cotton effect (sulfate concentration: 50 mM) The e...

Ngày tải lên: 19/02/2014, 05:20

... presence of EDTA The rough S minnesota LPS increased the initial fast phase of the reaction, but decreased the rate of the slow phase of oxidation in the presence of EDTA A comparison of rough and smooth ... reported that the oxidation of the a chains of Hb A0 was 10 times faster than that of the beta chains and that the oxidation of t...

Ngày tải lên: 08/03/2014, 10:20

... following nine mutations: V106A, V106G, V106H, V106I, V106K, V106L, V106M, V106Q and V106T We then characterized the enzymatic properties of the mutant enzymes The apparent native sizes of the –ATP ... of dTMP formation and thymidine concentration Open symbols, +ATP forms; closed symbols, –ATP forms (A) Dimeric enzymes: V106WT, V106A, V106I and V106T; (B) tetrameric enzymes:...

Ngày tải lên: 16/03/2014, 16:20

... the effect Mutations of yeast RNase H2 and archaeal RNase HII of a disease-causing mutation on the activity, stability, and structure of human RNase H2 and the role of the glycine residue fully ... or interactions with other proteins might be perturbed in human cells Alternatively, the mutations in the accessory proteins may not have...

Ngày tải lên: 17/03/2014, 17:20

... equilibrium of oxidation and chemical dissolution The dynamic equilibrium of the oxide formation (highly dependent on the acid concentration and availability of oxygen ions) and dissolution (relatively ... molecules once pass into one end (inner) of the pore would only come out from the other end (outer) of the pore without diffusing out from the middle of the pore- t...

Ngày tải lên: 19/03/2014, 16:48

... surface and the consequent effect on the structure of the protein Towards achieving this goal we studied the effect of ZnO NPs on the structure of ToxRp, alone and in the presence of denaturing ... changes of the ToxRp protein of V cholerae Based on the thermodynamic parameters of binding one can speculate on the nature of the interact...

Ngày tải lên: 23/03/2014, 03:20

... Ao and At are the intensities of the band of intact protein at the beginning of trypsinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig 8D) The apparent rate constants ... are same as given in (A) Point mutations of the b5–b7 loop of human HSP22 their secondary structure and on superposition of the mammal...

Ngày tải lên: 23/03/2014, 07:20

... representing the strongest effect on the 11bhydroxylation activity of all single mutants investigated here When both mutations were introduced into CYP11B2, the 11b-hydroxylation capacity was additionally ... strongly improve the substrate conversion in relation to the CYP11B2 wild-type protein (Fig 3) By replacing the amino acids in positions 112 and 147 of CYP1...

Ngày tải lên: 24/03/2014, 03:21