... Ala eliminated activity in binding to a BPA molecule, and individual mutations drastically reduced the activity Because Ala lacks the characteristic side chains of Glu and Arg, the mutant receptors ... A in human ERRc TGGTGGTTA-3¢ (xxx ¼ gcg for Glu275Ala, cgg for Glu275Arg, gac for Glu275Asp, and ctg for Glu275Leu); 5¢-TCCTTGGTGTCGTATACxxxTCTCTTTCA-3¢ (xxx ¼ gcg for Arg316 fi Ala, aag for Arg316 ... data indicate that Arg316 is crucial in exhibiting biological activity as well as in ligand -binding In the case of the mutant receptor (275Ala)-ERRc, with approximately 40% of the activity of...