... elevation of PCO content of erythrocytes treated with 2mM of primaquine by about 115% in relation to either control erythrocytes or erythrocytes treated with pravastatin On the other hand, erythrocytes ... 15 and the absorbance was measured at 532 nm Quantification of MDA levels was performed using tetraethoxypropane as the standard Determination of erythrocytes hemolysi...
Ngày tải lên: 25/10/2012, 11:40
... their health risks, and that health and educational professionals must mount new efforts to develop culturally appropriate and sensitive health programs and educational materials But, first, how physically ... between physical activity and health in women? Can a strong case be made for increasing physical activity in women as a primary preventive measure for major ch...
Ngày tải lên: 13/02/2014, 08:20
Tài liệu Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987-2006) docx
... al.: Time trends in leisure time physical activity and physical fitness in elderly people: 20 year followup of the Spanish population national health survey (1987 -200 6) BMC Public Health 201 1 ... prevent physical inactivity and improve the health status of older people in Spain List of abbreviations PA: Physical activity; LT...
Ngày tải lên: 14/02/2014, 06:20
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... 2011 FEBS S Cuyvers et al Secondary substrate binding in GH11 xylanases Table The effect of genetic engineering of the secondary binding site of the B subtilis and A niger xylanases on the screening ... ª 2011 FEBS 1101 Secondary substrate binding in GH11 xylanases S Cuyvers et al Table Biochemical characterization of B subtilis and A niger xylan...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... in a time-dependent enhancement of the 57-kDa band with a concomitant diminution of the 38-kDa and 19-kDa bands, as analyzed by SDS ⁄ PAGE (Fig 3A) The 38-kDa and 19-kDa bands did not disappear ... government works A Okubo et al A Kumamolisin-As with an engineered active site B C Fig PAGE analyses of the wild-type and mutants of kumamolisin-As Arrows indicate the...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx
... difluoro-substitutions at the ortho and meta positions on P1/P1¢-benzyloxy side groups of symmetric diol-based protease inhibitors preserve the binding efficacy and significantly improve the antiviral potency ... inhibitors complexed to HIV protease Compared with the nonsubstituted analog, the fluoro inhibitors have improved the antiviral activity...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... a biphasic loss of activity (Fig and Table 3) The phenotype of these substitutions is therefore different from that of N139A, T146A and W141A The F residues at positions 100 and 128 are highly ... incubation for 40 The specific inhibitory activity of PAI-1 at the various timepoints, i.e the fraction of the total amount of PAI-1 forming a stable complex with...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... protease activity in aging rats Fig Lon protease quantification and activity in liver mitochondrial matrix from 10- and 27-month-old rats Activity was determined in the absence of ATP or in the presence ... Protein alteration in mitochondrial matrix with aging (Eur J Biochem 270) 2299 Western blotting of modified proteins Fig Determination of CML-protein co...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf
... used as reference (set to d p.p.m.) All spectra were recorded at a temperature of 300 K and standard Bruker pulse programs were used in all experiments Spectra were recorded from a solution of ... C trachomatis L2 and E were nearly identical, whereas the LPS of Chl psittaci exhibits a second set of ions representing molecular species with four Kdo residues (m/z 2000) The...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationship between protein stabilization by osmolytes and its consequent ... no increase in catalytic efficiency in the presence of this group of osmolytes Interestingly, there is a linear relationship between DDGD°...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
... triplicate The data are reported as percentage of the value obtained by incubation of Hpt alone, and expressed as mean ± SEM A single representative of at least three independent experiments is ... respectively After incubation, the cell were lysed for measurement of their radioactivity and protein concentration The amount of cholesterol internalized by the cel...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt
... mutations from both the stability and activity mutants, and demonstrated a significant improvement in both stability and catalytic activity (Fig 1) As such, the activity and stability mutation combinations ... Rha et al Directed evolution of tyrosine phenol-lyase Table Genetic and catalytic changes of S toebii TPL during evolutionary engineering Library...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot
... 6.5 65 (a 2) 180 (a 4) 220 (a 4) 63 57 90 80 135 232 (a 4) 35 61 155 32 59 330 (a 8) 40 38 93 114 52 45 124 (a 6) 30 205 (a 4) 207 (a 4) 190 (a 4) 180 (a 4) 160 (a 6) 312 (a1 0) 133 (a 4) 36 98 (a 2) 52 83 77 ... thermodynamics (i.e for endothermic reactions) would result in increased yields when the reaction is performed at high temperatures; (e)...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... TGGACGGATATTGAAGCTGATCTCACCATAAAGGAT ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC ... be other meiotic clade AAA ATPases and have the AAA domain helix and the C-terminal helix, but not the b domain The distinguishing featu...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... hMnSOD has a low level of product inhibition, similar to that of the parent mutant Q143A hMnSOD, while exhibiting higher catalytic activity and efficiency Although even higher catalytic activity would ... efficiency and similarly low product inhibition compared with the Q143A hMnSOD parent Our results demonstrate the ability of directed evolution to engineer variants...
Ngày tải lên: 07/03/2014, 11:20