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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... protein, additional information about post-translational modifications, like 2895 Signal peptidase I activity in M pneumoniae Fig SIGNAL P predicted and experimentally verified cleavage site for P40 ... N-terminal part of the peptide, it is evident that the N-terminal amino acid is asparagine (position 26), but increased in mass by 136 Da Because this modification is only possible at the free a amino ... although a SPase I activity has been shown to be essential for cell viability in all bacteria analyzed [26] To test experimentally whether there is a type I SPase activity in M pneumoniae, we determined...

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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx

... high-af®nity binding proteins (IGFBPs) with  10 -fold higher af®nity than their binding to IGF receptors IGFBPs thereby in¯uence the availability of IGF to bind to IGF receptors [12 ] Over the past 14 years, ... analogue binding to highanity recombinant IGF1 R Binding of IGF- II (A), des- (1 6) -IGF- II (B) and Arg6 -IGF- II (C) to rhIGF1R measured by BIAcore analysis using concentrations of 25, 50, 10 0, 200 and ... af®nity binding of IGF- I, IGF- II and their analogues to rhIGF1R was measured by BIAcore analysis (Figs and 2), and relative binding af®nities were determined (Table 1) Arg3 -IGF- I, des- (1 3) -IGF- I,...

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