... http://www.biomedcentral.com/147 1-2 229/11/37 Page of 14 ** * Canina-2 -H19, -H20 Canina-1 Canina-1 -H13 Canina-1 -H19 Canina-1 -H20 Gallica Woodsii Rugosa Woodsii -H19 Figure Haplotype network of nrITS-1 Haplotype ... of these alleles in the remaining plants we performed allele-specific PCRs according to the conditions described above using the forward primers (LFYin1-al4-fwd: 5’-GGACATGTAAATAGGTCGAGAATATAT-3’, ... biotinylated primers Anal Biochem 2006, 356(2):19 4-2 01 doi:10.1186/147 1-2 22 9-1 1-3 7 Cite this article as: Ritz et al.: To be or not to be the odd one out Allele-specific transcription in pentaploid...