Biomass modelling: Estimating thermodynamic properties from the elemental composition

... at the bottom of the cleft and a lobe containing the structural zinc at the mouth of the cleft and thus exposed to the solvent [8] Gradual depletion of the enzyme first of the structural and then ... expression of the glucose dehydrogenase gene of P torridus in Escherichia coli, the biochemical characterization of its product and the effect of zinc ion...

Ngày tải lên: 19/02/2014, 16:20

... using the residual water signal as internal reference [49] Modelling the thermodynamic parameters The model used for the thermodynamic characterization of DvHc3 [13] was applied to the data obtained ... solutions In the reduced and intermediate 2257 Thermodynamic parameters in ligated proteins stages of oxidation the pH was adjusted inside the anaerobic glove box wit...

Ngày tải lên: 19/02/2014, 17:20

... UV-VIS and FT-IR spectroscopic properties of the caa 3oxidase from T thermophilus in the presence and absence of cyanide, and compare the observed properties to previous reports on members of the heme ... between cytochrome c and cytochrome c oxidase Previous reports on the caa3- oxidase from T thermophilus concluded that this enzyme is a typical...

Ngày tải lên: 21/02/2014, 15:20

... histidines (His67 and His247, presumably providing metal binding at site II and the neighbouring His242) bind phosphate ions, thereby providing another level of competition for metal ion binding Kinetics ... modelling of Cu(II) and Ni(II) transport by albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at sit...

Ngày tải lên: 22/02/2014, 04:20

... structure and prior distributions Description of the databases The analysis database included mortality, weather and air pollution data for the 20 largest metropolitan areas in the USA for the 7-year ... average, maximum and 8-h maximum, we used the 24-h mean for each day If a city has more than one weatherstation, we took the average of the measurements from...

Ngày tải lên: 06/03/2014, 16:20

... exposed to the solvent (Fig S5B) Resolution of the structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side of the isoalloxazine ring, and that the 2Â-P-AMP ... claimed that His126 of A fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivativ...

Ngày tải lên: 07/03/2014, 02:20

... the same conditions, these apparent values can be used for comparison of binding afnities of the inhibitors under study The tting of the binding isotherms of all ve compounds with a binding model ... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitrogen of Asn114Â of the other subunit The...

Ngày tải lên: 07/03/2014, 11:20

... resonance study of the role of nitric oxide and catalase in the activation of guanylate cyclase by sodium azide and hydroxylamine Modulation of enzyme responses by heme proteins and their nitrosyl derivatives ... progress The evidence from the spectroscopic and ligand-binding studies of chlorite dismutase suggest binding of chlorite to the five-co-ordi...

Ngày tải lên: 08/03/2014, 16:20

... tokodaii; Ap, Aeropyrum pernix; Ta, Thermoplasma acidophilum; Tv, Thermoplasma volcanium; Fa, Ferroplasma acidarmanus; Tm, Thermotoga maritima; Aa, Aquifex aeolicus; Tt, Thermoanaerobacter tengcongensis ... presence of unlabeled ATP, indicating that ATP and the analog 8-azido-ATP recognize the same binding site The ATPase activity of PfPDO was demonstrated The hydrolysis of ATP...

Ngày tải lên: 16/03/2014, 16:20

... properties of [NiFe]-hydrogenases [27,41] The interaction of H2 with hydrogenases offers the possibility to study redox changes in enzyme in the absence of redox mediators simply by varying the H2-partial ... are not suited for the study of the Fe–S clusters of Ech As the membranes contain only very low amounts of other hydrogenases, the Niu* signal is consider...

Ngày tải lên: 17/03/2014, 10:20

... signal The intensity of this signal strongly varied from preparation to preparation A decrease in specific activity was always accompanied by an increase in signal intensity, suggesting that the ... characterization of the Fe-only nitrogenase isolated from R capsulatus, which included a detailed comparison with the Mo- containing nitrogenase from the same or...

Ngày tải lên: 18/03/2014, 01:20

... polarity of the environment of the fluorophore In the case of PfLDH, the signal change on binding to the active site was too small to be used as a reliable reporter of the formation of the binary ... that the KM is controlled by a single ionizing group with a pK of 7.95 ± 0.08, similar to other lactate dehydrogenases of this mechanistic family [9] Transient kine...

Ngày tải lên: 23/03/2014, 09:20

... studied the thermal unfolding of both the wild-type HUTmar protein and its E34D mutant in acidic solutions (pH 4.00, 3.75 and 3.50) to improve the reversibility of unfolding and avoid postunfolding ... We report here the results of an extensive thermalstability study of a hyperthermophilic protein, the histonelike protein from T maritima (HUTmar-wt...

Ngày tải lên: 23/03/2014, 12:20

... liver and four other organs of the spadefoot toad Scaphiopus couchii [19] In the present study, two GST isoforms were purified from liver of the anoxia tolerant turtle, T s elegans Analysis of kinetic ... hydroperoxides and aldehydes These may be the primary substrates of the microsomal or membrane-bound GST in the same way as they are the substrates for Se-depe...

Ngày tải lên: 23/03/2014, 15:20

... CbHs CdHs CeHs CfH NH CaH CbH CdHs CeHs NH CaH CbH CdH NH CaH CbH CdHs CeHs CfH NH CaH CbH CbHÂ CdHs CeHs OH NH CaH CbH3 NH CaH CbH CcH3 NH CaH CbH CbHÂ CcH CdH3 CdH3Â NH CaH CbH3 NH CaH CbH ... NH CaH CbH CbHÂ NdH NH CaH CbH CcH3 NH CaH CbH CcH3 CcH3Â NH CaH CbH CbH CdHs CfH NH CaH CaHÂ NH CbH CbHÂ CdHs CeHs CfH NH CaH CbH CdHs CeHs CfH NH CaH CbHs CcH CeH3 CaH CbH CcHs CcHÂ CdH3 3.25 ... po...

Ngày tải lên: 23/03/2014, 17:22