Token passing protocols

Ngày tải lên: 26/06/2016, 21:12

Ngày tải lên: 21/08/2012, 16:18

... Terminal H.323 cña Cisco Locall Area Network Intererface Admission Control With Cisco IOS MCM Gatekeeper – Call Across WAN May I call 2111 with a bandwidth of 384K? Call flow H.323 RAS signaling ... you can! IP WAN zone zone Admission Control Via Zone BandWidth in the Cisco IOS MCM Gatepeer H×nh4: Admission - Cisco A B Setup Alerting Connect TermCapSet TermCapAck mSDet mSDetAck mSDetConfi...

Ngày tải lên: 24/08/2012, 21:11

Ngày tải lên: 13/09/2012, 10:51

... different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; ... correct volume of 10 Davies and Carlstedt ice-cold dry propan-1-ol to give a 100 mM solution DFP is unstable in water but can be stored at –20°C in propan-1-ol After dilution, the vial as w...

Ngày tải lên: 23/09/2012, 19:25

... to T-antigen (β-d-Gal 1-3 GalNAc), but also to structures found within the backbone of oligosaccharides (β-d-Gal 1-3 / 4GlcNAc) (19) Demonstration of PNA binding is not necessarily evidence of T-antigen ... Blue 8GX in 0.1 N HCl (pH 1.0) HID: Dissolve 120 mg of N,N-dimethyl-m-phenylenediamine dihydrochloride (Sigma) and 20 mg of N,N-dimethyl-p-phenylenediamine dihydrochloride (Sigma) in 5...

Ngày tải lên: 23/09/2012, 19:25

... nonionic detergents: 0.05% (v/v) Nonidet P-40 or Tween-20 Enzyme substrates: 2,2'-azino-bis(3-ethylbenzathiazaline 6-sulfonic acid) (ABTS) (1 mg/mL, A405nm), O-phenyldiamine (OPD) (1 mg/mL, A492nm), ... chromogens that leave a colored precipitate on the blot (e.g., 3,3'-diaminobenzidine, 4-chloronapthol mix for HRP [14]; 5-bromo-4-chloro-3'-indolyphosphate toluidine salt, nitro blue tetrazoli...

Ngày tải lên: 23/09/2012, 19:25

... 250 mg of phenol in 70 mL of glycerol Poly-L-lysine-coated slides: slides are immersed in 0.01% (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of ... A., Allen, A., Morris, E., and Ross-Murphy S B (1988) Mucus glycoprotein gels: role of glycoprotein polymeric structure and carbohydrate side-chains in gel-formation Carbohydrate Res 178, 93–1...

Ngày tải lên: 23/09/2012, 19:25

... as [35S]sulfate-labeled, TCA-precipitable proteins (cpm/mL) (see Note 10) g Mature [35S]sulfate-labeled, mucin band on 4% SDS-PAGE (au/mL) h Total concentration of mucin by dot-blotting (au/mL) ... before use, from 100 mM stock solution in 2-propanol 21 Blotto (wash buffer for Western-type dot-blots): 50 mM Tris (pH 7.8), mM CaCl2, 0.05% (v/v) Nonidet P-40 (BDH), 0.01% antifoam A (Sigma), 5%...

Ngày tải lên: 23/09/2012, 19:25

... advisable We analyze aliquots of the peaks by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and purify peptides to homogeneity by rechromatography ... effectiveness of the separation is monitored with a periodic acid-Schiff (PAS) assay and with a variety of lectins and mucin- or carbohydrate-specific antibodies (for a discussion of the rela...

Ngày tải lên: 23/09/2012, 19:26

... alternative, however, is rate-zonal centrifugation, which separates molecules not on hydrodynamic volume alone but on their mass-to-volume ratio as well The basis of rate-zonal centrifugation for ... Distribution of Gel-Forming Mucins 89 Fig Schematic diagram of gradient-forming apparatus We next describe an experiment that involves the simultaneous preparation of × 12 mL (in 13-mL tubes) 6–8...

Ngày tải lên: 23/09/2012, 19:26

... of mucin glycoproteins The hydrolysate of mucin is then derivatized with Fmoc on the α-amino groups under alkaline conditions The derivatized amino acids are separated by a C18 reversed-phase column ... continuous flow of helium Hypersil C18 reversed-phase column: 150 × 4.6 mm inner diameter µm (Keystone, Bellefonte, PA) In-line filter (2 µm) (Upchurch, cat no 10 0-1 0) 2.2 Chemicals 2.2.1...

Ngày tải lên: 23/09/2012, 19:26