Adaptive evolution of a duplicated pancreatic ribonuclease gene in a leafeating monkey

... Enzyme activities of recombinant RNASE1B, RNASE1 and mutant forms of RNASE1 a, RNase activity against yeast tRNA at different pH levels b, RNase activity against dsRNA Mutant forms of douc langur ... 2.5 RNase activity against dsRNA (OD/min/nmol) Using statistical analysis of nucleotide substitutions and biochemical assays of recombinant proteins, we have described the adaptive...

Ngày tải lên: 27/05/2016, 21:11

... However, in all four patients, we saw very few non-synonymous mutations and a comparable frequency of synonymous mutations in both genes This analysis is technically challenging because it involves the ... seen at the HLA-B*57 restricted epitope KF9 (Nef 82-90) in ES3 and ES8 (Figure 1) In both patients, the majority of the plasma clones were different at or amino acids...

Ngày tải lên: 13/08/2014, 01:20

... we discuss the evolution of fish egg envelope genes using phylogenetic analysis Results Purification of envelope proteins from Japanese eel The unfertilized egg envelope proteins of Japanese eel ... (ML) trees of the nucleotide sequences of the zp genes of Teleostei (A) Phylogenetic tree of teleostean zp genes The subfamilies of zp genes are...

Ngày tải lên: 15/03/2014, 23:20

... catalytic domain of the human HAT P ⁄ CAF, without affecting its catalytic activity or substrate specificity P ⁄ CAF, p300 ⁄ CBP-associating factor, is a trans criptional coactivator with a variable N-terminal, ... Continuous HAT assay Progress curves as a measure of HAT activity of wild-type and mutant P ⁄ CAFs were followed using a coupled enzyme assay [62] HAT a...

Ngày tải lên: 23/03/2014, 06:20

... wild-type and mutant Pseudomonas SY-77 glutaryl acylase on glutaryl- 7-ACA and adipyl- 7-ADCA Shown are values of mean ± S.D of at least four independent measurements Ó FEBS 2002 4500 C F Sio et al (Eur ... other hand, the mutants are not improved in catalysis of glutaryl- 7-ACA as shown by the lower kcat of all mutants and the lower affinity of mutant Y178H for the glutaryl...

Ngày tải lên: 31/03/2014, 09:20

... which are of the orders of 1/N, 1, and N Hence, the amplitude A can be approximately obtained as A= a (N + 1) a sin θs (A. 9) O Kukrer and A Hocanin The covariance σXY can be obtained as σXY ... constrained poles and zeros,” IEEE Transactions on Acoustics, Speech, and Signal Processing, vol 33, no 4, pp 983–996, 1985 [6] P Stoica and A Nehorai, “Performance analysis of an a...

Ngày tải lên: 22/06/2014, 23:20

... Comparisons of CENP-C proteins in animals, yeast and plants The CENPC motif and conserved regions found at the termini of CENP-C proteins are indicated For pairwise comparisons of protein-coding sequences, ... DNAbinding proteins would maintain an interface with the conserved kinetochore machinery Indeed, regions of CENP-C that show evidence of positive selection inclu...

Ngày tải lên: 06/08/2014, 18:21

... pinpointing the genetic and phenotypic attributes that may explain the emergence of the hypervirulent 027 strain and contribute in general to our understanding of the evolution of bacterial virulence Materials ... [22] The genomic sequences of strains CD196 and R20291 were compared against the database of strain 630 proteins by blastx, and a CDS featu...

Ngày tải lên: 09/08/2014, 20:20

... morbidity and mortality HIV-1 infected children account for 20% of all HIV-1 related deaths; 7% of individuals living with HIV-1 infection, and 16% of new HIV-1 infections annually [4] In sub-Saharan ... homogeneity, and rapid viral replication kinetics, have been correlated with perinatal HIV-1 transmission [6-8] In addition, advanced maternal disease status, lack of dr...

Ngày tải lên: 13/08/2014, 09:21

... within a single animal can vary by greater than 35% at the aa level The ranges of aa diversity in some intra-host pairwise SIVsm V1V2 sequence comparisons in this study rival that of inter-animal ... glycosylated in naturally infected SMs Presumably, continually evolving antibody responses in these natural hosts maintain a highly glycosylated surface protein, albeit with...

Ngày tải lên: 13/08/2014, 09:21

... optimal care of trauma patients As early as 1966 the role of physicians staffing emergency rooms in the care of trauma patients was discussed as an important element in preventable trauma death ... training allows exposure to trauma only as part of their general EM training, particularly if a separate trauma team responds for trauma activations In smaller...

Ngày tải lên: 13/08/2014, 23:20