... EhADH2, indicating the presence of two catalytically independent domains, the N-terminal domain, displaying ALDH activity, and the C-terminal domain, containing an iron-binding domain, which is involved ... buffer and incubated for h on ice, and the remaining EhPFOR activity was determined A control experiment was prepared with a sample diluted in N2-purged buffer, and incubated for h on ice in the ... bifunctional Entamoeba histolytica alcohol dehydrogenase (EhADH2) protein is necessary for amebic growth and survival and requires an intact C-terminal domain for both alcohol dehydrogenase and...