Molecular mechanisms underlying the thermal stability and acid induced unfolding of CHABII

... curves of CHABII and [Phe21] -CHABII at pH 6.5 (b) Thermal unfolding curves of CHABII and [Phe21] -CHABII at pH 4.0 (c) Thermal unfolding curves of [Phe21] -CHABII at pH 4.0 and [Phe21] -CHABII at ... proteins Extensive 32 CD and NMR experiments were performed to characterize the role of His21 in the pH -induced unfolding of CHABII Thermal unfoldin...

Ngày tải lên: 26/11/2015, 12:54

... in the session of “Inflammatory Mechanisms in Rhinosinusal Disease”.) iv Molecular mechanisms underlying the pathogenesis of nasal polyposis and its response to steroid treatment Content of the ... through the epithelial defect and the mucosa tends to cover it by migration of the epithelium from the edges 23 of the defect; (iii) the pr...

Ngày tải lên: 10/09/2015, 15:49

... spatial and temporal resolution In this thesis, I have studied the molecular mechanisms that regulate the determination of the position of the cleavage furrow and the cleavage furrow formation in cytokinesis ... action Inhibit myosin II ATPase activity by binding to myosin II-ADP-Pi Blebbistatin and inhibit the release of the ADP Inhibit actin pol...

Ngày tải lên: 14/09/2015, 14:09

... as: Qin and Tian: Exploring the molecular mechanisms underlying the potentiation of exogenous growth hormone on alcoholinduced fatty liver diseases in mice Journal of Translational Medicine 2010 ... mRNA expression HMW adiponectin is the major bioactive isoform of adiponectin, and is responsible for the insulin-sensitizing effects of adiponectin, whil...

Ngày tải lên: 18/06/2014, 16:20

... antithrombins are glycosylated at four of their asparagine molecules; these are of the α isoform The remaining 5–15% of circulating antithrombins, of the β isoform, lack glycosylation at asparagine ... purposes) Interaction of antithrombin with endothelium The affinity of antithrombin (AT) to thrombin and its enzymatic inhibition is increased by binding of AT with t...

Ngày tải lên: 12/08/2014, 23:21

... symptoms of envenomation and the ultimate severity of a bite depend on the size and species of the snake, the amount and degree of toxicity of the venom injected, Introduction the location of the ... MOLECULAR MECHANISMS UNDERLYING THE BIOCHEMICAL ACTIVITIES OF NAJA SPUTATRIX VENOM CHARMIAN CHER DYI NI B.Sc (Hons) A THESIS SUBMITTED FOR THE DEGR...

Ngày tải lên: 16/09/2015, 08:31

... FprA unfolding A Trp Emm Max (nm) temperature For native FprA, a broad sigmoidal transition between 30 and 65 °C having an apparent Tm (mid point of thermal denaturation) of  49 °C and a loss of ... presence of increasing concentrations of GdmCl In (A) circles and squares represent data for native and 0.2 M CaCl2-stabilized FprA, respectively changes in enzy...

Ngày tải lên: 19/02/2014, 16:20

... relation to learning and memory formation requirements Furthermore, the molecular mechanisms underlying the MFs redistribution during learning and memory formation are still under investigation ... hilus and CA3 area, comprise the second synapse of the hippocampal circuit In this trisynaptic model of the hippocampus, based on the observations of th...

Ngày tải lên: 09/09/2015, 18:55

... 5.2.3.1 Fear conditioning training 115 5.2.3.2 Short-term contextual memory and cued memory test 115 5.2.3.3 Retention and extinction of long-term contextual memory and cued memory ... which have been done in this thesis to investigate the underlying mechanisms of learning process and long-term memory formation In part I, two projects have been focused on...

Ngày tải lên: 09/09/2015, 18:55

... potassium phosphate buffer Unfolding of the wild-type Cel7A (s) and the pH mutant enzyme (h) at (A) pH 5.8 and (B) pH 8.0 Analysis of heat denaturation of the wild-type and mutant protein at pH 5.8 ... 8.0 and 25 °C Spectra of the wild-type Cel7A at pH 5.8 (d) and pH 8.0 (s), and the pH mutant at pH 5.8 (j) and pH 8.0 (h) were recorded...

Ngày tải lên: 31/03/2014, 07:20

... HANDLED HANDLED 40 35 30 25 20 15 10 C A2 C A2 Figure Effects of handling on MR and GR immunoreactivity in the CA2 region of the hippocampus Effects of handling on MR and GR immunoreactivity ... assay system) Statistical Analysis Data were analyzed by a one-way analysis of variance (ANOVA) with handling as the independent factor Data on learning, body weight and...

Ngày tải lên: 08/08/2014, 21:20

... et al.: Potential mechanisms underlying the acute lung dysfunction and bacterial extrapulmonary dissemination during Burkholderia cenocepacia respiratory infection Respiratory Research 2010 11:4 ... characterized by bacterial extrapulmonary dissemination and systemic inflammatory response [15] The mechanisms that permit bacteria to disseminate are not...

Ngày tải lên: 12/08/2014, 14:20

... transitions of the nucleotide -binding cleft For this purpose, we studied the effects of the D -loop cleavage on the thermal unfolding of G- and F -actin Previously, the thermal unfolding of G -actin containing ... assume therefore that the decrease in the thermal stability observed by DSC on actin species cleaved within the D -loop is associated with...

Ngày tải lên: 06/03/2014, 22:21

... both SHP-1 promoters are transcriptionally active in hematopoietic and epithelial cells, we used RT-PCR to specifically amplify either the (I )SHP-1 or (II )SHP-1 transcripts Expression of (I )SHP-1 ... used in PCR for (I )SHP-1 with primer pair (I )SHP-1- 90-5¢ and SHP-1- 1859-3¢, or (II )SHP-1 with primer pair (II )SHP-1- 74-5¢ and SHP-1- 1859-3¢ The RT-PCR products were separated...

Ngày tải lên: 08/03/2014, 23:20

... by DSC to be the Tm of the protein These fibrils are amyloidogenic, as determined from their Stability and fibril formation of TTR and TRP tinctorial properties in the ThT-binding and CR-binding ... with the thermostability of its tetrameric and monomeric structures Thermostability and protein structures We have analyzed the structures of hTTR (Protein...

Ngày tải lên: 16/03/2014, 01:20