Novel mechanisms of programmed cell death in the protozoan parasite blastocystis

... proteins specialized in DNA repair Interestingly, they also contain motifs typical of proteins involved in RNA metabolism TgDRE mRNA and protein expression have been evaluated using RT-PCR and ... many new avenues of future investigations into the unexplored molecular mechanisms involved in DNA repair and RNA metabolism in the protozoan parasites such as T g...

Ngày tải lên: 08/03/2014, 22:20

... signalregulated kinase ⁄ (ERK1 ⁄ 2) and protein kinase B (PKB) pathways that act downstream of oncogenic protein kinases [10,11] It is increasingly apparent that BIM as a mediator of tumour cell ... a potent inhibitor of mitogen-activated protein kinase ⁄ extracellular signal-regulated kinase kinase ⁄ kinases: mechanism of action in vivo, pharmacokinetic ⁄ pharmacody...

Ngày tải lên: 16/03/2014, 00:20

... out the findings on the principles of confidentiality, anonymity and non-remuneration in the donation of reproductive cells, as well as donor compensation, consent for egg cell donations and the ... donor offers the cells exclusively for the use of the recipient specified in the declaration of donation; c) the declaration of donation includes – beyon...

Ngày tải lên: 28/03/2014, 16:20

... transfection (Fig 6E) 10 1 FL2-H 10 2 10 3 10 1 LQ: 3% 10 0 10 1 FL2-H 10 2 10 3 UQ: 97% LQ: 97% 10 0 10 2 10 3 FL1-H 10 4 10 1 D 10 0 Fig Analysis of ANT1 -silencing on DW dissipation and the effect of the mitochondrial ... ANT1 -silencing According to this hypothesis, we found that ANT1 siRNA-treated cells 10 2 FL1-H 10 3 10 4 10 3 10 4 UQ: 86% 10 3 10 4 L...

Ngày tải lên: 29/03/2014, 09:20

... square areas containing specific bands and an identical blank area drawn in the immediate vicinity of the band, according to the following formula: Band 1/Band ratio= (Band 1-blank)/ (Band -blank) ... marginal ability to invade, the presence of β2m clearly induced significant migratory activity (Fig 6A) Evaluation of the surface area of the scratch at and 24 hours time p...

Ngày tải lên: 18/06/2014, 15:20

... important role in the secondary activation of microglia, in the progression of the inflammatory response, and in the progressive loss of the dopaminergic neurons in MPTPinduced PD Therefore, COX-2 ... oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining of the init...

Ngày tải lên: 19/06/2014, 22:20

... nature of goblet cell carcinoids, the location of hormones and the phenotype of endocrine cells Another useful method for simultaneous revealing of mucin secretion and endocrine cell component of the ... with the hypothesis of the dual entodermal and neuroendocrine origin of goblet cell carcinoid [1] The ultrastructural investigation showed that...

Ngày tải lên: 09/08/2014, 07:21

... little emphasis in the past The objective was to determine the mode of lymphocyte cell death upon exposure to the gaseous phase of cigarette smoke (GPS) in vitro For this purpose, we utilised a well-established ... significantly different (p < 0.62) FACS analysis of Annexin V/PI-stained cells To determine the mode of cell death upon GPS treatment,...

Ngày tải lên: 12/08/2014, 14:20

... the molecular mechanisms underlying latency in the monocyte-macrophage lineage is poor Like in CD4+ T cells, two types of latency occur in cells from the monocyte-macrophage lineage Page of 16 ... cells of the monocyte-macrophage lineage However, several mechanisms generating HIV-1 post-integration latency have been described in the monocyte-macrophage,...

Ngày tải lên: 12/08/2014, 23:23

... with only a variance in their longitudinal center of gravity Computer Systems Analysis of the Hypothesis The hypothesis was examined through a systems analysis approach using a derivative of a well-established ... comprehensive theory of understanding [14] The analysis suggested that changes in the capacitance of the lower extremity veins secondary to a...

Ngày tải lên: 13/08/2014, 16:20

... set There are 1,364 and 1,323 gene pairs in human and mouse, respectively, in the FAM2 gene set deposited research TAGs are tandemly arrayed genes that belong to the same gene family There are ... of the human gene pairs and 91% of the mouse gene pairs in the NEW gene set have Ks ≤ 0.25, which confirms the recent duplications of these gen...

Ngày tải lên: 14/08/2014, 08:20

... regulatory questions in the actively multiplying tachyzoite, as any given population of cells in culture consists of parasites at different points of their cell cycle Our study reports the presence of ... a majority of the bases in each motif were substituted by base-specific transversions, thus destroying the original sequence of the candidate motif but maintainin...

Ngày tải lên: 14/08/2014, 21:20

... despite the many morphological and biochemical studies of PCD in protozoan parasites, most of the homologs of mammalian molecules involved in cell death signaling are missing in the protozoa and the ... Furthermore, by dissecting the mechanisms and regulation of the staurosporine-induced cell death pathway in Blastocystis may lead to discovery of n...

Ngày tải lên: 16/10/2015, 15:35