Structure determination of midkine a

... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide...

Ngày tải lên: 14/02/2014, 19:20

... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design o...

Ngày tải lên: 18/02/2014, 06:20

... interactions established in the active site is also of relevance The active site: structural and electrostatic basis of HtA function The reaction catalyzed by ribotoxins follows a mechanism of transphosphorylation, ... Pozo A & Gavilanes JG (2001) RNase U2 and alphasarcin: a study of relationships Meth Enzymol 341, 33 5–3 51 ´ Lacadena J, Alvarez-Garcı´ a...

Ngày tải lên: 18/02/2014, 08:20

... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- C...

Ngày tải lên: 19/02/2014, 00:20

... Cavada et al PPL2, an endochitinase from Parkia platycephala Fig Crystal structure of PPL2 (A) and (B) show two views of the (ab)8 barrel fold of PPL2 The a- helices (red) and b-strands (yellow) are ... The Authors Journal compilation ª 2006 FEBS 3963 PPL2, an endochitinase from Parkia platycephala B S Cavada et al Fig Purification and molecular mass d...

Ngày tải lên: 16/03/2014, 13:20

... by a nearest-neighbour thermodynamics calculation, suggesting that it can be useful for evaluating the reopening temperature for a DNA hairpin in a local region of DNA, and for comparing the reopening ... measuring the reopening temperature for a single type of hairpin structure in DNA has been established The reopening temperature obtained exp...

Ngày tải lên: 23/03/2014, 13:20

... fatty acid; no information was available about the fatty acid distribution on the proximal GlcN Analysis of intact lipid A and ammonium hydroxide treated lipid A fractions The negative ion MALDI-TOF ... The compositional analysis revealed the presence of Kdo and hydroxy fatty acids, typical components of LPS SDS/PAGE revealed a ladder-like pattern typical of an S-f...

Ngày tải lên: 24/03/2014, 00:21

... kDa, a second peak of 30 kDa and a third peak of about 20 kDa A similar gel filtration experiment was also carried out for the complex of XAIP with a- amylase A mixture of XAIP and a- amylase was ... observations indicate that XAIP associates with GH11 xylanase and GH13 a- amylase, as well as with both xylanase and a- amylase simultaneously Tissue distribution...

Ngày tải lên: 29/03/2014, 09:20

... H,Y., Saxena, A. M and Swaminathan, K (2006) Crystallization and preliminary X-ray diffraction analysis of Salmonella typhi PilS ACTA Cryst F 62: 1024-1026 Crystal structure of Salmonella typhi ... CRYSTAL STRUCTURE ANALYSIS OF PILS, A TYPE IVB PILIN FROM SALMONELLA TYPHI MANIKKOTH BALAKRISHNA ASHA (B.Sc., B.Ed., M.Sc.) A THESIS SUBMITTED FOR THE DEG...

Ngày tải lên: 14/09/2015, 10:57

... Founded 1905 CRYSTAL STRUCTURE DETERMINATION OF HEX1 AND HUMAN GEMININ7 0 -152 YUAN PING (B.S., M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR AND CELL BIOLOGY ... Function test of Geminin7 0 -152 ···················································97 3.2.3 Expression and purification of Geminin7 0 -152 ··················...

Ngày tải lên: 17/09/2015, 17:20

... CRYSTAL STRUCTURE DETERMINATION OF KREMEN1, DICKKOPF1 AND MeCP2 VINDHYA B.N.REDDY A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES ... INTERACTION OF DKK-1/KRM/LRP5/6 DNA METHYLATION 11 1.5.1 Methyl-CpG binding proteins (MeCP2) 12 1.5.2 Structure of MBDs 13 1.5.3 MeCP2 15 1.5.4 Architecture of MeCP2 16 1.5.5 Role of...

Ngày tải lên: 04/10/2015, 10:23

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... cycle of refinement may go on several times before a high resolution structure can be determined Figure 1.5: Outline of the procedure for protein structure determinat...

Ngày tải lên: 04/10/2015, 15:45

... used to amplify the mdka gene The sense primer is 5’-CGCGGATCCAAAAACAAGAAAGAGAAGAA-3’ and antisense primer is 3’-GGGGATATCACTCAGCATAAT-5’ DNA gel electrophoresis (Agarose from Bio-Rad) was subsequently ... vertebrae and ribs, dermis of the back, and skeletal muscles of the back, body wall and limbs (Wikipedia) 1.1.2 Characterization of Mdka and Mdkb of zebrafish Fig1.2 Organization o...

Ngày tải lên: 16/10/2015, 15:35

... N-Acetyl aspartate (NAA), N-Acetylaspartylglutamate (NAAG), Alanine, Choline, Creatine, Glutamate, Glycine, Myo-inositol, Lactate, Pyruvate, Serine, Threonine, Tyrosine and Valine Acetate is an ... Magnetic resonance spectroscopy correlation with histological analysis in gliomas and structure determination of a hypothetical protein Xu Ying A THESIS SUBMITTE...

Ngày tải lên: 10/11/2015, 11:35