Characterization of liver x receptor and retinoid acid rece

Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

... Gevaert et al COFRADIC and protein modiﬁcations analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular applicatio...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with a His-tag and Snz1p ... SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clariﬁed Expression and puriﬁcation of Sno1p and Snz1p In light...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

... blue, hematine, and p-chloranil were obtained from ICN; hydroxyethyl disulfide (HEDS) and vinylene trithiocarbonate was obtained from Aldrich EST identification and cDNA isolation The S mansoni EST ... (lane 4) electroblotted and immunodetected by a-SmGSTO serum (B) RT-PCR analysis of different S mansoni stages Reverse-transcribed RNA from miracidia (lane 1), sporocysts (lane...

Ngày tải lên : 21/02/2014, 01:21

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Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... mansoni NR1 A B W Wu et al DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding ... assay in in vivo results Experimental procedures Parasites The NMRI strain of S mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesoc...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and ﬂux control analysis ppt

... objective of understanding how the glycolytic ux in E histolytica is controlled, we cloned the genes, and overexpressed, puried and determined the kinetic parameters of the 10 glycolytic enzymes ... reconstruction of the nal stages of amoebal glycolysis Analysis of the kinetic properties of the recombinant glycolytic enzymes indicated that EhPPDK and EhPG...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx

... 16a-hydroxyprogesterone Both expressed enzymes had no lyase activity towards PROG or 17-OHPROG Understanding of the complexity of hydroxylase and lyase activity of CYP17 by way of primary sequence ... the metabolism of PREG by the two enzyme preparations Despite the large degree of homology between the human and baboon CYP17 the expressed baboon CYP17 was una...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... puriﬁcation of the recombinant enzyme In order to construct an expression vector for Cl-idh, two oligonucleotides (sense, 5¢-AAAAACATATGGCAAGCA AATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAA AAGGATCCCGGCTGAAAACCGGGCTGCATTA-3¢) ... dehydrogenase is known to predominantly catalyze the reduction of oxaloacetate to malate, and thereby lowering the possibilities of oxaloacetate ac...

Ngày tải lên : 08/03/2014, 10:20

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Báo cáo khoa học: The HS:19 serostrain of Campylobacter jejuni has a hyaluronic acid-type capsular polysaccharide with a nonstoichiometric sorbose branch and O-methyl phosphoramidate group docx

... structure for the HS:19 serostrain and have shown that these labile groups are an a- l-sorbofuranose branch attached at C2 of b-d-GlcA and a MeOPN modiﬁcation located at C4 of b-d-GlcNAc There are very ... the disappearance of signals associated with the sorbose monosaccharide and the production of the same oxidation product as observed for the l -sorbos...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt

... haemagglutinin at the C-terminus and encoding the cathepsin D propeptide fused to mature cathepsin E, we have demonstrated that the propeptide of cathepsin E, likewise those of other aspartic proteinases, ... maturation of cathepsin E, we constructed a mutant lacking the propeptide (Leu23-Phe58) and expressed it in HEK -293T cells The transfected cells were pul...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae potx

... kept at °C and used within 10 days Quantification of alkaline phosphatase and aminopeptidase activities Specific alkaline phosphatase (ALP) and N-aminopeptidase (APN) enzymatic activities of BBMV ... sugar Canavalis ensiformis (ConA) a- Man a- Glc Galb1 fi 3GalNAc Galb1 fi 3,4GlcNAc a/ bGalNAc a/ bGal Galb1 fi 4GlcNAc Gala1 fi 3Gal GalNAca1 fi 3GalNAc GalNAca1 fi 3Gal Galb1 fi 3G...

Ngày tải lên : 23/03/2014, 13:20

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Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx

... We interpret this observation as the retainment of newly synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The ... lower (not shown) In order to determine the in uence of the reduction of the expression of TbPEX14 on the import of glycosomal matrix proteins,...

Ngày tải lên : 23/03/2014, 17:21

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university of calgary press space and spatial analysis in archaeology dec 2005

... space and spatial reckoning are inextricably culture- (and language-) bound, and a better understanding of space and the experience of living in space should be one of the primary goals of the ... Settlement and Urbanism: Proceedings of a Meeting of the Research Seminar in Archaeology and Related Subjects Held at the Institute of Archaeology, London Un...

Ngày tải lên : 11/06/2014, 16:57

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Báo cáo toán học: "A Hybrid of Darboux’s Method and Singularity Analysis in Combinatorial Asymptotics" potx

... Plan of the paper We start by recalling in Section the principles of two classical methods dedicated to coeﬃcient extraction in combinatorial generating functions, namely Darboux’s method and singularity ... at the singularity z = can be determined, resulting in (7) (The representation in (7) is in fact exact and not merely asymptotic It has been obtained by Zagier...

Ngày tải lên : 07/08/2014, 13:21

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Characterization of liver x receptor and retinoid acid receptor mediated response with transcriptome and histological analysis in zebrafish liver

... CHARACTERIZATION OF LIVER X RECEPTOR- AND RETINOID ACID RECEPTOR- MEDIATED RESPONSE WITH TRANSCRIPTOME AND HISTOLOGICAL ANALYSIS IN ZEBRAFISH LIVER HENDRIAN SUKARDI NATIONAL UNIVERSITY OF SINGAPORE ... Transcriptomic response to liver X receptor (LXR) agonist T0901317 in zebrafish liver 28 3.1 29 3.2 3.3 3.4 Histological analysis of T0...

Ngày tải lên : 03/10/2015, 20:32

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