Identifying key signatures of high productivity from transcriptome and proteome profiles of CHO cells

... produced from these cells (Anderson and Krummen, 2002; Chu and Robinson, 2001) To meet market demands, the scale of bio-therapeutic production is usually very large, often in tens of thousands of litres, ... plausible that the gene and protein expression profile of a highly productive cell line holds the key signature for high productivity, we used combined transcripto...

Ngày tải lên: 09/10/2015, 11:06

... modifications on heparin binding, recombinant CHO cells were treated with inhibitors of tyrosine sulfation and N-glycosylation under conditions that allowed partial inhibition of these modifications ... In summary, we present evidence for a very strong similarity of HCII from human serum and its recombinant counterpart from CHO cells with respect to tyrosine...

Ngày tải lên: 08/03/2014, 22:20

... extensive and systematic analysis of the genome and proteome composition of halophilic organisms, along with a comparative study of non-halophiles, with a view to characterize the molecular signatures ... and frequency distribution of genes on hydrophobicity and pI COA on amino acid usage and frequency distribution of genes on hydrophobicity and pI (a) Positions...

Ngày tải lên: 14/08/2014, 08:21

... and Adam Pauls 2006a Multi-Document Summarization of Evaluative Text In Proceedings of the conference of the European Chapter of the Association for Computational Linguistics Giuseppe Carenini, ... to combine multiple specifications to include all possible features Clustering algorithm can be used to combine specifications We propose an approach that takes following inherent...

Ngày tải lên: 20/02/2014, 09:20

... ensure the generation of the ketone The ability of ScPDC and ZmPDC to decarboxylate indolepyruvate was examined under the same conditions In the case of ZmPDC maximum enzyme concentration was 2.3 ... independent of the concentration of the auxiliary enzyme, confirming that the coupled assay monitors the true rate of EcIPDC catalysis Figs and and Table illustr...

Ngày tải lên: 08/03/2014, 02:20

... determination and cloning of the cDNA encoding two novel peptides, SrIA and SrIB, found in the venom of Conus spurius The pattern and the spacing of their cysteines indicate that they belong to the a-Conotoxins ... In the present article, we have defined the weak antagonist properties of the novel C spurius a-conotoxins, and of a synthetic analog...

Ngày tải lên: 16/03/2014, 11:20

... complex from carbonatewashed membranes of SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex ... assembly and stabilization of the complex [50–52] High levels of PS complex were detected in mouse embryonic brain consistent with high expres...

Ngày tải lên: 16/03/2014, 16:20

... Cite this article as: Ahmed et al.: Neuronal transcription factor Brn-3a(l) is over expressed in high-grade ovarian carcinomas and tumor cells from ascites of patients with advanced-stage ovarian ... pattern of Brn-3a(l) in primary tumors, ascites tumor cells and ovarian cancer cell lines consistent with novel distinct role of this...

Ngày tải lên: 20/06/2014, 07:20

... Several data are available on the respiration of Mediterranean ecosystems Most of them were measured in the Mediterranean Basin [4, 5, 10, 15, 26, 32, 37, 38] Some other data dealing with Australian ... that the increase of temperature has a greater impact on the global respiration of the ecosystem Topsoil moisture and CO2 efflux than on primary productio...

Ngày tải lên: 08/08/2014, 01:21

... et al.: Diagnostic accuracy of existing methods for identifying diabetic foot ulcers from inpatient and outpatient datasets Journal of Foot and Ankle Research 2010 3:27 Submit your next manuscript ... of Foot and Ankle Research 2010, 3:27 http://www.jfootankleres.com/content/3/1/27 Page of Table Comparison of methods for diagnostic accuracy of...

Ngày tải lên: 10/08/2014, 21:24

... gAgTTggATgggTCCTCTgC CCAAACCAAAgCCAgTTTCATTCA CCAggTTTTgTATgAgTgCCgTA ACCTTggCCATCCTCTTCTT AgAAATCTTgACCCCCgTTC AAggAgCTCTTgACgTTggA TgCTAACAggTgggAAAACC CCTTCCAgCAgATgTggATT AgATTAggCAAggCgAggAT BEC87-GSP1 ... TgATTTTAgCTgCATgTgCACCTgAgAA CgTCATggAAATgTCTTAATTggCTTgCTg gAAgAAAACAAATTgggAggAggAgAA gCgTgTTCCAAAgAACACAATTCAgTgCCTT BEC-32BamHI DX24BamHI ggATCCTgATCTgTggATTgggTTTCgTgg ggATCCgggTg...

Ngày tải lên: 12/08/2014, 03:21

... epidemiology and clinical features Gastric cancer etiology 21 The African and Asian enigma 50 Possible molecular pathogenetic attributes of gastric cancer 66 Translocations in epithelial tumors and their ... balanced translocation in IM95 10 Dissecting complex rearrangements: integrating spectral 235 karyotypes and whole genome copy number profiles 10.1 Global in...

Ngày tải lên: 14/09/2015, 08:38

... it.' Prepositions after adjectives characteristic of independent of typical of We hope that the examples will help you find your way around in grammar books so that you can successfully organise ... the basic message of the sentence.) Goethe, who died in 1832, was a famous writer Our car, which is quite old, is still reliable I'd like you to meet our secretaries, some of whom ar...

Ngày tải lên: 05/10/2012, 09:58

... number 629 Genotyping of Clostridium perfringens isolated from cattle and pigs with diarrhea in Hanoi and surrounding areas, Vietnam 3.2 Genotyping of C perfringens the toxicity of C perfringens ... Genotyping of Clostridium perfringens isolated from cattle and pigs with diarrhea in Hanoi and surrounding areas, Vietn...

Ngày tải lên: 29/08/2013, 08:14

... surveyed anti-cyanobacterial activities of the plant extracts These findings indicates the possible release of anti-cyanobacterial compounds from vegetation in artificial floating islands, and such ... Hishikaki, Japan) Confirmation of the release of anti-cyanobacterial allelochemicals In order to confirm the release of anti-cyanobacterial allelochemicals,...

Ngày tải lên: 05/09/2013, 09:38