Engineering dengue virus NS3 protease for structural studies

... Bibliography……………………………………………………………… 77 VII SUMMARY Dengue virus (DENV) NS3 protease (NS3pro) is essential for viral polyprotein processing, a critical component of viral replication NS3pro is a serine protease and comprises ... Nuclear localization sequence Non -structural protein Nucleoside triphosphatase NS3hel NS3pro OG PCR pI Flavivirus NS3 helicase domain Flavivirus NS3...

Ngày tải lên: 05/10/2015, 21:23

... at the P1′ position of the dengue virus cleavage site, whereas the preferred P1′ residue of the WNV NS3 protease is G [19-21] Theoretical molecular interactions between the active site of the ... G133 in the formation of the oxyanion hole [25] A map of the dengue virus NS3 protease active site with residues putatively involved in enzyme-s...

Ngày tải lên: 10/08/2014, 05:21

... Pacific and the Americas A substantial number of people travelling to endemic regions are also infected each year In the last year, DHF has again been on the increase in India and in several Asian ... Melino and M Paci Progress for dengue virus diseases Table Vaccines against dengue fever by the Initiative for Vaccine Research, World Health Organization, updated Februar...

Ngày tải lên: 19/02/2014, 00:20

... been reported for the WNV NS2B–NS3 protease The poor quality of the NMR spectrum of WNV NS2B–NS3pro in the absence of inhibitors is reminiscent of the situation in the homologous NS2B–NS3pro construct ... 2009 The Authors Journal compilation ª 2009 FEBS 4253 NMR analysis of the West Nile virus protease 10 11 12 13 14 15 16 17 18 X.-C Su et al minant...

Ngày tải lên: 07/03/2014, 02:20

... inuences the catalytic activity, as both its kcat and Km values remain lower than those of activated 1C zymogen The ratio of the (kcat Km )activated value to the (kcat Km)unactivated value provides ... unactivated (d) and activated (s) 1C zymogens assessed by CD (A) Near-UV CD spectra of unactivated and activated 1C zymogens (0.5 mgặmL)1 in NaCl Pi) (B) Thermal den...

Ngày tải lên: 07/03/2014, 11:20

... refer to the sequences that are present in the genome of interest and absent from the host genome as being ‘host-blind’ (human- blind, mosquito-blind, mouseblind, rat-blind, etc.) sequences The greater ... (22-mers) of the viral genome is comprised of n-mers that are not present in any of the other dengue genomes For example, in the genome o...

Ngày tải lên: 23/03/2014, 11:20

... Journal 20 05, 2: 26 http://www.virologyj.com/content /2/ 1 /26 Figure Ultrastructural features of DEN2 virus- infected monocytes Ultrastructural features of DEN2 virus- infected monocytes A) DEN2 virus- infected ... Journal 20 05, 2: 26 http://www.virologyj.com/content /2/ 1 /26 Figure Electron1microscope morphological observations of DEN2 virus particles Electron microsco...

Ngày tải lên: 19/06/2014, 08:20

... outbreak in northern India in 2003 and its continued dominance again in 2004 indicates the resurgence of dengue- 3 in a dominant form The emergence of a newer dengue serotype after an interval ... III) The reemergence of highly fatal subtype III of DEN-3 in a dominant form, replacing the earlier circulating subtype IV of DEN-2 in India is a matter of great concern...

Ngày tải lên: 20/06/2014, 01:20

... classification for dengue severity The new classification for dengue severity is divided into Dengue without Warning Signs, Dengue with Warning Signs, and Severe Dengue 24 1.2 Molecular Biology of DENV ... Fisher and Prof Leo Yee Sin, thank you for recruiting patients for our study To Prof Mary Ng and Boon, thank you for providing us with technical advice and...

Ngày tải lên: 09/09/2015, 08:16

... BIOLOGY OF DENGUE VIRUS 1.2.1 Taxonomy of dengue virus Dengue virus (DENV) belongs to the family of Flaviviridae that consists of three genera, flavivirus (e.g dengue virus, West Nile virus, and ... BIOLOGY OF DENGUE VIRUS 1.2.1 Taxonomy of dengue virus 1.2.2 Structure and genetic organization of dengue virus 1.2.3 Viral infection cycle 1.2.4...

Ngày tải lên: 10/09/2015, 15:51

... ESTABLISHING IMPROVED PLATFORMS FOR DENGUE DIAGNOSIS AND HYBRIDOMA DEVELOPMENT USING DENGUE ENVELOPE DOMAIN III ANTIGEN Melvin Tan Lik Chern B.Sc (Hons), Monash University, Australia A THESIS ... envelope protein comprises regions: Domain I, Domain II and Domain III (Fig 1.1B) Domain I is the central domain, Domain II is the dimerization and...

Ngày tải lên: 10/09/2015, 15:53

... (2007) The envelope < /b> glycoprotein domain < /b> III < /b> of dengue < /b> virus < /b> serotypes and < /b> inhibit virus < /b> entry Microbes and < /b> Infect 9, 1-6 Chiu, M.W & Yang, Y.L (2003) Blocking the dengue < /b> virus < /b> infections on BHK-21 ... envelope-< /b> specific Mabs are able to block virus < /b> entry into cells (Crill & Roehrig, 2001) Mabs that are specific...

Ngày tải lên: 10/09/2015, 15:53

Ngày tải lên: 11/09/2015, 09:56

... 1.1.5.4 Virus assembly and propagation 17 1.1.6 Phylogeny of Dengue Virus 19 1.1.7 Pathogenesis of Dengue Virus 22 1.1.8 Immune response to Dengue Virus 26 1.1.8.1 Innate immunity to DV 26 1.1.8 .2 ... culture 62 2.8 Source of primary CD 22+ B cells 63 2. 9 RNA extraction and RT-PCR for serotyping of patients 63 2. 10 Cloning of B cells from Dengue virus- i...

Ngày tải lên: 11/09/2015, 09:56