Crystallographic studies on geminin CDT1 complex, proteins involved in DNA replication

... GBD-hCdt1 binds strongly with RID-hGeminin - 67 3.7 Crystallization of Cdt1 /Geminin complex .- 68 Chapter4 Discussions - 71 4.1 Identifying the Geminin Binding Domain of hCdt1 ... ORC CDT1 Pre-RC S phase (Activation) CDKs Geminin (Licensing) Figure2.1 DNA replication licensing control by Geminin and CDKs during the cell cycle In metazoa, Geminin, present from the onset ......

Ngày tải lên: 04/10/2015, 10:24

... proteins where zinc is complexed through four invariant cysteine and/or histidine residues, forming a zinc finger domain that is mostly involved in DNA binding but also in protein–protein interactions ... the coupling of zinc- binding structures (either in MT or in zinc finger proteins) by selenium compounds with the cellular redox system GSH/GSSG, reducibl...

Ngày tải lên: 30/03/2014, 15:20

... protein molecules and their interactions with DNA in real-time reactions and the dynamics of their DNA- binding Therefore, my objective of finding the characteristics of protein binding to single DNA ... crucial role in meiosis; it synapses DNA into a stable formation in a zinc dependent manner Overall, my studies have provided important insight of four DNA bin...

Ngày tải lên: 09/09/2015, 11:25

... declined due to production constraints such as lack of production inputs and lack of information on dairy and beef production and marketing and also the dairy and beef market are localized Therefore, ... the production constraints of milk and meat of the woreda and to indicate the interventions for the indicated production constraints LITERATURE REVIEW 2....

Ngày tải lên: 08/03/2014, 23:20

... domains for its adaptor function, the importin b binding (IBB) domain in its N-terminus and the C-terminal NLSbinding domain In the absence of importin b, an auto-inhibiting part of the IBB domain ... in maintenance of the normal homeostatic function of cells We identified and characterized two types of monopartite nuclear localization sequences of D melanogaster prote...

Ngày tải lên: 15/03/2014, 11:20

... screen with human 14-3-3 f as bait and isolated clones for two proteins involved in distinct neurodegenerative diseases Results Identification of d-catenin as a 14-3-3 interacting protein The yeast ... range of interacting proteins and diversity of neurodegenerative disorders 4207 14-3-3 in neurodegenerative disease in which isoforms of 14-3-3 are implicated, in...

Ngày tải lên: 16/03/2014, 22:20

... Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase- related proteins Plant Physiol 137, 762–778 Kamauchi S, ... with His-Bind resin (EMD Biosciences, Inc.) After washing of the column with binding buffer containing 60 mm imidazole, recombinant proteins were eluted with binding buff...

Ngày tải lên: 23/03/2014, 09:21

... http://www.translational-medicine.com/content/7/1/75 Figure Protein Tyrosine Kinase and other Major Kinases involved in Angiogenic Pathways Protein Tyrosine Kinase and other Major Kinases involved in Angiogenic ... serine-threonine kinases, lipid kinases, adhesion molecules and other diffusible signaling proteins The abundance of multiple PTKs and other kinase...

Ngày tải lên: 18/06/2014, 15:20

... mechanism in root-cap peripheral cells in which the Atkinesin-13A and ALA3 play essential roles Conclusion In this paper we found that AtKinesin-13A located on Golgi-associated vesicles in Arabidopsis ... observation showed that AtKinesin-13A was located on Golgiassociated vesicle in root cap cells of Arabidopsis AtKinesin-13A was labeled with the purif...

Ngày tải lên: 12/08/2014, 03:21

... this article as: Bussone et al.: Identification of < /b> new < /b> autoantibody < /b> specificities < /b> directed < /b> at < /b> proteins < /b> involved < /b> in < /b> the < /b> transforming < /b> growth < /b> factor < /b> b pathway in < /b> patients with systemic sclerosis ... 4) Thus, the < /b> expression of < /b> these proteins < /b> can be either increased or d...

Ngày tải lên: 12/08/2014, 15:23

... mediated by a TRIM5-Cyclophilin A fusion protein generated by retroposition [28] Evolutionary analysis of PPIA indicates that Cyclophilin A has been preserved by strong purifying selection, leaving ... interdisciplinary research from the Faculty of Biology and Medicine of the University of Lausanne (to A.T and H.K.) References Yang Z: The power of phylogenetic comparison in reveal...

Ngày tải lên: 13/08/2014, 09:21

... tool for protein targeting to answer existing questions pertaining to fission yeast mitotic regulation and cytokinesis Several aspects need to be investigated regarding the positioning and assembly ... requirement of F-actin for cytokinesis in fission yeast (Balasubramanian et al., 1992) Additional isolation and analyses of fission yeast mutants defective in cyt...

Ngày tải lên: 09/09/2015, 11:21

... data for each domain and the remaining 1600 labeled reviews in each domain are used as training data All unlabeled data are used for latent representation learning For computational reason, only ... Recently, there is increasing research interest in the application of machine learning methods for business analytics (Abbasi and Chen 2008, Rui and Whinston 2011) an...

Ngày tải lên: 09/09/2015, 11:28

... mechanisms using in vitro mammalian cell models The following investigations have been conducted: (i) examining the role of autophagy in zVAD-induced necrosis by modulation of autophagy via either pharmacological ... CONCLUSIONS 163 5.1 AUTOPHAGY PLAYS A PRO-SURVIVAL ROLE IN ZVAD-INDUCED NECROSIS 5.2 ZVAD INHIBITS AUTOPHAGY VIA SUPPRESSION OF CATHEPSIN ACTIVITY...

Ngày tải lên: 11/09/2015, 10:17

... FUNCTIONAL STUDIES ON SULPHATION STATUS OF HEPARAN SULPHATE IN BREAST NON- TUMOURIGENIC EPITHELIAL AND CANCER CELLS GUO CHUNHUA (B.Med., M.Med.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Undersulphation of GAGs inhibited invasion of breast cancer cell in vitro 120 Discussion 123 HSPG and breast cancer growth 123 HSPG and adhesion,...

Ngày tải lên: 12/09/2015, 08:18