CRYSTALLISATION AND PRELIMINARY STRUCTURAL ANALYSIS OF ECM18 WHICH CATALYSES DISULFIDE TO THIOACETAL CONVERSION IN ECHINOMYCIN BIOSYNTHESIS

... Triostin A and echinomycin are represented in sticks Triostin A is coloured in pink and echinomycin in cyan; (b) Superimposition of the crystal structures of triostin A and echinomycin Minor deviations ... is on the structural study of the enzyme Ecm18 involved in the final step of the biosynthetic pathway of echinomycin 1.3.1 Biosynthesis of echinomyc...

Ngày tải lên: 04/10/2015, 10:23

... Triostin A and echinomycin are represented in sticks Triostin A is coloured in pink and echinomycin in cyan; (b) Superimposition of the crystal structures of triostin A and echinomycin Minor deviations ... is on the structural study of the enzyme Ecm18 involved in the final step of the biosynthetic pathway of echinomycin 1.3.1 Biosynthesis of echinomyc...

Ngày tải lên: 12/10/2015, 17:36

... plot of nuclear NF-jB from in vitro and in silico analysis of the data The plot shows nuclear NF-jB oscillation in the original model and in the updated model with newly measured kr1, ka1 and ... described for the kinase time-course assay The assay was again repeated with the inclusion of 10 mm MnCl2 in the kinase condition In vitro analysi...

Ngày tải lên: 16/03/2014, 11:20

... identification and simulation A mathematical model was developed, representing central carbohydrate metabolism in leaves of A thaliana The model was based on the following system of ordinary differential ... in A thaliana A D B E C F Fig Maximum activities of enzymes in central carbohydrate metabolism during cold exposure (A C) Vmax values of three invertase...

Ngày tải lên: 14/02/2014, 22:20

... b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343) On the analogy of the soybean b-amylase, the active site of the ... for the b-amylase from C sepium using the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c...

Ngày tải lên: 22/02/2014, 07:20

... we have purified, cloned and characterized Cyn d 24 as a novel pathogenesis-related protein from BGP Additionally, the identification of Cyn d 24 has identified the involvement of a novel class of ... AGAD AADA.NA.VG D. D 113 Zea AYA.S A- QRQG LI GG FW AGAD.SASDA.GS.VS QY.DHDT.S 112 Nicotiana AYA.N S-Q.AA NL HGQ AE -GDFMTAAKA.EM.V QY.DHD 118 Cyn d 24 DQGKM...

Ngày tải lên: 07/03/2014, 12:20

... mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated ... LPS (R4 core-type) is as depicted in Fig Structural analysis of E coli F653 core-oligosaccharides (R3 core) We have subjected purified LPS from E c...

Ngày tải lên: 08/03/2014, 09:20

... of NHase seems to form a disulfide bond easily under oxidative conditions, if the site contains no metal ion The metal activator of NHase may incorporate a metal ion to prevent the formation of ... & Endo, I (1999) Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a liga...

Ngày tải lên: 16/03/2014, 16:20

... dehydrogenase and prephenate dehydratase catalyze the biosynthesis of tyrosine and phenylalanine, respectively As this biosynthetic pathway is absent in mammals but is essential for the survival of bacteria ... Reddy PT (2006) Biochemical and structural characterization of the secreted chorismate mutase (Rv1885c) from Mycobacterium tuberculosis H37Rv: an *Ar...

Ngày tải lên: 17/03/2014, 17:20

... Cite this article as: Mueser et al.: Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives Virology Journal 2010 ... duplex DNA likely explains the sensitivity of T4 ligase activity to salt concentration Conclusion and Future Directions of Structural Analys...

Ngày tải lên: 11/08/2014, 21:21

... and attachment of pea seeds to the replum in a pod of the def mutant pea The def mutant pea shows a swollen and thick funicle compared to the wild type Arrows indicate the AZ and ALZ in the wild ... growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing t...

Ngày tải lên: 12/08/2014, 03:20

... residues, the other of tyrosines This grouping is based on the similar characteristics of serine and threonine, and the fact that they are usually targeted by the same protein kinases The study focused ... identity cut-off (>30%) very few sites were highly conserved (Figure 7a) Only less than 5% of the sites were strictly conserved across the alignments, and...

Ngày tải lên: 14/08/2014, 07:21

... vertebrate BTB- ZF and BBK proteins are more closely related, and cannot be separated by BLASTCLUST Long form of the BTB domain The majority of BTB domains from the BTB- ZF, BBK, MATHBTB, RhoBTB and BTB- basic ... some BTB- ankyrin proteins are composed of an amino-terminal BTB domain, a central helical region, 19 ankyrin repeats and a carboxy-terminal FYVE domain...

Ngày tải lên: 14/08/2014, 14:22

... R G IQLR L FYNVT YT AYRLS P L FGF EYSNL TEIG KKL TRF L RY GTDRT GYFTN STRF LLHKQ WT LYSLT P L YKF SYSN- - L KEY SR L L NA FIVAE KQ K G LAVE V LLHKQ WT IYSLT P L YKF SYSN- - L KDY SR L L SA ... SSRTEFFISALLNYGLYKD FTLRDLLRPDP RRLVQVLSALINFLYYRD YTYMDIIKPA VKKTLATLSYLFNHLAYYK 40% 3% 10% 26% scNdc80 Fungi Metazoa Plantae Newly annotated protein QELLEYLTHNNFELEMKHSLGQNTLRSP TQKDFNYIFQWLYHRIDPGYRF...

Ngày tải lên: 14/08/2014, 16:21

... disassembly Gelsolin/ villin superfamily severs actin filaments Twinfilin inhibits actin nucleotide exchange Actin/Bundling/Crosslinking Proteins α-actinin connects and organizes actin filaments EPLIN ... N-terminal half of gelsolin During apoptosis, caspase-3 cleaves gelsolin between domains and 4, yielding a calcium- independent, threedomain, actin-severing protein (Kothakota et...

Ngày tải lên: 11/09/2015, 10:17